scholarly journals A Replica Plating Technique for the Isolation of Nutritionally Exacting Mutants of a Filamentous Fungus (Aspergillus nidulans)

1959 ◽  
Vol 20 (3) ◽  
pp. 540-548 ◽  
Author(s):  
C. F. ROBERTS
Keyword(s):  
Aspergillus Nidulans ◽  
Filamentous Fungus ◽  
Plating Technique ◽  
Replica Plating

scholarly journals A technique for replica platingCoprinus lagopus, a filamentous fungus

1971 ◽  
Vol 17 (3) ◽  
pp. 279-281 ◽  
Author(s):  
Philippa J. Kaplan ◽  
D. Walls
Keyword(s):  
Filamentous Fungi ◽  
Filamentous Fungus ◽  
Sodium Deoxycholate ◽  
Regular Pattern ◽  
Plating Technique ◽  
Replica Plating

SUMMARYThe problem of replica plating filamentous fungi such asCoprinus lagopusis overcome by inducing micro-colonies with sodium deoxycholate and using ‘Velcro’, a hooked material, to replace velveteen in the standard replica plating technique. ‘Velcro’ is advantageous in that it has a regular pattern of closely spaced hooks which transfer small inocula from the colonies on the master plate.

REPLICA PLATING TECHNIQUE FOR STUDYING MICROBIAL INTERACTIONS IN SOIL

1965 ◽  
Vol 11 (4) ◽  
pp. 629-636 ◽  
Author(s):  
G. Stotzky
Keyword(s):  
Nutritional Composition ◽  
Mixed Population ◽  
Microbial Interactions ◽  
Individual Species ◽  
Selective Inhibitors ◽  
Subsequent Growth ◽  
Sterile Soil ◽  
Plating Technique ◽  
Replica Plating ◽  
Plating Method

A replica plating method was developed to study ecology of microorganisms in soil. Precise placement of inocula and amendments at desired loci in sterile soil contained in petri plates were accomplished with a template. Subsequent growth and distribution of individual species, even when part of a mixed population, was measured by periodic transfer with an easily constructed replicator to agar plates of differing nutritional composition or containing selective inhibitors. The method is rapid and reproducible, and permits the study of many variables and interactions in a single soil plate; it can also be used with non-sterile soil and other suitable microbial habitats.

Sign in / Sign up

Export Citation Format

Share Document