The Gibberella fujikuroi niaD Gene Encoding Nitrate Reductase: Isolation, Sequence, Homologous Transformation and Electrophoretic Karyotype Location

ABSTRACT Anaerobic cultures of Shewanella oneidensis MR-1 grown with nitrate as the sole electron acceptor exhibited sequential reduction of nitrate to nitrite and then to ammonium. Little dinitrogen and nitrous oxide were detected, and no growth occurred on nitrous oxide. A mutant with the napA gene encoding periplasmic nitrate reductase deleted could not respire or assimilate nitrate and did not express nitrate reductase activity, confirming that the NapA enzyme is the sole nitrate reductase. Hence, S. oneidensis MR-1 conducts respiratory nitrate ammonification, also termed dissimilatory nitrate reduction to ammonium, but not respiratory denitrification.

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Protein accumulation and expression of the nitrate reductase gene in Spirulina platensiscells depending on the spectral composition of led radiation

Proceedings of the National Academy of Sciences of Belarus Biological Series ◽

10.29235/1029-8940-2019-64-2-180-189 ◽

2019 ◽

Vol 64 (2) ◽

pp. 180-189

Author(s):

N. V. Kozel ◽

M. S. Radyuk ◽

T. V. Samovich ◽

I. A. Dremuk ◽

L. S. Gabrielyan

Keyword(s):

Nitrate Reductase ◽

Spectral Composition ◽

Dry Weight ◽

Protein Accumulation ◽

Control Analysis ◽

Nitrate Reductase Gene ◽

Gene Encoding ◽

Led Lighting ◽

Reductase Gene ◽

Red Leds

The effect of LED lighting of different spectral composition on the productivity of Spirulina platensis, an accumulation of protein in alga cells and an expression of the nitrate reductase gene has been studied. It was shown that LED lighting with a predominance of the red component in the emission spectrum allows achieving 9–29 % higher alga productivity compared to using fluorescent lamp illumination. Illumination with single blue light resulted in significant (83 %) decrease in the productivity of Spirulina platensiswhich apparently was due to the absence of the yellow and red components in the illuminator spectral composition, which are most effectively absorbed by phycocyanin. A positive correlation between an increase in the productivity of alga and the accumulation of protein in its cells was found. So, by using an illuminator with red LEDs, the protein content increased by 21 % calculated per gram of dry weight and 47 % calculated per liter of suspension relative to the control. Analysis of the expression of the Nar gene encoding nitrate reductase in Spirulina platensiscells did not reveal a direct dependence between an increasing protein accumulation and an expression level of the Nar gene in the most promising in terms of biomass and protein yield sample of alga, growing under red LEDs. This indicates the crucial role of the photosynthetic activity of Spirulina platensiscells in increasing productivity and protein synthesis.

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Disruption of narG, the Gene Encoding the Catalytic Subunit of Respiratory Nitrate Reductase, Also Affects Nitrite Respiration in Pseudomonas fluorescens YT101

Journal of Bacteriology ◽

10.1128/jb.181.16.5099-5102.1999 ◽

1999 ◽

Vol 181 (16) ◽

pp. 5099-5102 ◽

Cited By ~ 18

Author(s):

Jean-François Ghiglione ◽

Laurent Philippot ◽

Philippe Normand ◽

Robert Lensi ◽

Patrick Potier

Keyword(s):

Nitrate Reductase ◽

Pseudomonas Fluorescens ◽

Reductase Activity ◽

Anaerobic Growth ◽

Gene Encoding ◽

Α Subunit ◽

Regulatory Link ◽

Nitrite Respiration ◽

Nitrate And Nitrite ◽

Respiratory Nitrate Reductase

ABSTRACT The Pseudomonas fluorescens YT101 genenarG, which encodes the catalytic α subunit of the respiratory nitrate reductase, was disrupted by insertion of a gentamicin resistance cassette. In the Nar− mutants, nitrate reductase activity was not detectable under all the conditions tested, suggesting that P. fluorescens YT101 contains only one membrane-bound nitrate reductase and no periplasmic nitrate reductase. Whereas N2O respiration was not affected, anaerobic growth with NO2 as the sole electron acceptor was delayed for all of the Nar− mutants following a transfer from oxic to anoxic conditions. These results provide the first demonstration of a regulatory link between nitrate and nitrite respiration in the denitrifying pathway.

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Cloning and expression of a gene encoding a root specific nitrate reductase in bean (Phaseolus vulgaris)

Physiologia Plantarum ◽

10.1111/j.1399-3054.1991.tb00081.x ◽

1991 ◽

Vol 82 (2) ◽

pp. 197-204 ◽

Cited By ~ 29

Author(s):

Tine Hoff ◽

Bjarne M. Stummann ◽

Knud W. Henningsen

Keyword(s):

Phaseolus Vulgaris ◽

Nitrate Reductase ◽

Cloning And Expression ◽

Gene Encoding

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Maximal Expression of Membrane-Bound Nitrate Reductase inParacoccus Is Induced by Nitrate via a Third FNR-Like Regulator Named NarR

Journal of Bacteriology ◽

10.1128/jb.183.12.3606-3613.2001 ◽

2001 ◽

Vol 183 (12) ◽

pp. 3606-3613 ◽

Cited By ~ 45

Author(s):

Nicholas J. Wood ◽

Tooba Alizadeh ◽

Scott Bennett ◽

Joanne Pearce ◽

Stuart J. Ferguson ◽

...

Keyword(s):

Nitrate Reductase ◽

Mutational Analysis ◽

Paracoccus Denitrificans ◽

Regulatory Function ◽

Gene Encoding ◽

Iron Sulfur Cluster ◽

Oxygen Sensitive ◽

Membrane Bound ◽

Paracoccus Pantotrophus ◽

Maximal Expression

ABSTRACT Respiratory reduction of nitrate to nitrite is the first key step in the denitrification process that leads to nitrate loss from soils. In Paracoccus pantotrophus, the enzyme system that catalyzes this reaction is encoded by the narKGHJI gene cluster. Expression of this cluster is maximal under anaerobic conditions in the presence of nitrate. Upstream from narKis narR, a gene encoding a member of the FNR family of transcriptional activators. narR is transcribed divergently from the other nar genes. Mutational analysis reveals that NarR is required for maximal expression of the membrane-bound nitrate reductase genes and narK but has no other regulatory function related to denitrification. NarR is shown to require nitrate and/or nitrite is order to activate gene expression. The N-terminal region of the protein lacks the cysteine residues that are required for formation of an oxygen-sensitive iron-sulfur cluster in some other members of the FNR family. Also, NarR lacks a crucial residue involved in interactions of this family of regulators with the ς70subunit of RNA polymerase, indicating that a different mechanism is used to promote transcription. narR is also found inParacoccus denitrificans, indicating that this species contains at least three FNR homologues.

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