Sphingomonas gei sp. nov., isolated from roots of Geum aleppicum
A yellow-pigmented bacterium, designated strain ZFGT-11T, was isolated from roots of Geum aleppicum Jacq. collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. Cells of strain ZFGT-11T were Gram-stain-negative, strictly aerobic rods that were surrounded by a thick capsule and were motile by means of a single polar flagellum. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZFGT-11T was a member of the genus Sphingomonas and was closely related to Sphingomonas naasensis KACC 16534T (97.6 % similarity), Sphingomonas kyeonggiense JCM 18825T (96.8 %), Sphingomonas asaccharolytica IFO 15499T (96.7 %) and Sphingomonas leidyi DSM 4733T (96.6 %). The predominant respiratory quinone was ubiquinone-10 (Q-10) and the major cellular fatty acids were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C17 : 1ω6c, C14 : 0 2-OH, C16 : 0 and C15 : 0 2-OH. The major polyamine of strain ZFGT-11T was sym-homospermidine. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, sphingoglycolipid, two unidentified aminoglycolipids, two unidentified phospholipids and two unidentified lipids were detected in the polar lipid profile. The DNA G+C content was 66.8 mol%. DNA–DNA relatedness for strain ZFGT-11T with respect to its closest phylogenetic relative S. naasensis KACC 16534T was 26.2±4.8 % (mean±sd). On the basis of data from the present polyphasic taxonomic study, strain ZFGT-11T is considered to represent a novel species of the genus Sphingomonas , for which the name Sphingomonas gei sp. nov. is proposed. The type strain is ZFGT-11T ( = CCTCC AB 2013306T = KCTC 32449T = LMG 27608T).