scholarly journals Larkinella bovis sp. nov., isolated from fermented bovine products, and emended descriptions of the genus Larkinella and of Larkinella insperata Vancanneyt et al. 2006

2011 ◽  
Vol 61 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Rangasamy Anandham ◽  
Soon-Wo Kwon ◽  
Hang-Yeon Weon ◽  
Soo-Jin Kim ◽  
Yi-Seul Kim ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
Major Respiratory Quinone ◽  
Motile By Gliding

A novel bacterial strain, designated M2T2B15T, was isolated from fermented bovine products and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Cells were strictly aerobic, Gram-reaction-negative, oxidase- and catalase-positive rods that lacked flagella and were motile by gliding. Flexirubin-type pigments were absent. 16S rRNA gene sequence analysis indicated that strain M2T2B15T was related most closely to Larkinella insperata LMG 22510T (94.4 % similarity) but shared <87 % similarity with other members of the phylum Bacteroidetes. The major cellular fatty acids were C16 : 1 ω5c, iso-C15 : 0 and iso-C17 : 0 3-OH. The polar lipids were phosphatidylethanolamine, phosphatidylserine, two unidentified aminophospholipids and two unidentified polar lipids. Menaquinone 7 (MK-7) was the major respiratory quinone. The G+C content of the DNA of strain M2T2B15T was 52 mol%. The phenotypic, genotypic and phylogenetic data presented clearly indicate that strain M2T2B15T represents a novel species of the genus Larkinella, for which the name Larkinella bovis sp. nov. is proposed. The type strain is M2T2B15T (=KACC 14040T =NBRC 106324T). Emended descriptions of the genus Larkinella and of Larkinella insperata Vancanneyt et al. 2006 are also proposed.

scholarly journals Sphingobacterium daejeonense sp. nov., isolated from a compost sample

2006 ◽  
Vol 56 (9) ◽  
pp. 2031-2036 ◽  
Author(s):  
Kyoung-Ho Kim ◽  
Leonid N. Ten ◽  
Qing-Mei Liu ◽  
Wan-Taek Im ◽  
Sung-Taik Lee
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
The Family

A Gram-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated TR6-04T, was isolated from compost and characterized taxonomically by using a polyphasic approach. The organism grew optimally at 30 °C and at pH 6.5–7.0. The isolate was positive for catalase and oxidase tests but negative for gelatinase, indole and H2S production. Comparative 16S rRNA gene sequence analysis showed that strain TR6-04T fell within the radiation of the cluster comprising Sphingobacterium species and clustered with Sphingobacterium mizutaii ATCC 33299T (96.7 % sequence similarity); the similarity to sequences of other species within the family Sphingobacteriaceae was less than 92.0 %. The G+C content of the genomic DNA was 38.7 mol%. The predominant respiratory quinone was MK-7. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c). These chemotaxonomic data supported the affiliation of strain TR6-04T to the genus Sphingobacterium. However, on the basis of its phenotypic properties and phylogenetic distinctiveness, strain TR6-04T (=KCTC 12579T=LMG 23402T=CCUG 52468T) should be classified as the type strain of a novel species, for which the name Sphingobacterium daejeonense sp. nov. is proposed.

scholarly journals Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae

2005 ◽  
Vol 55 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Seung Bum Kim ◽  
Suk Kyun Han ◽  
Cindy Snauwaert ◽  
Marc Vancanneyt ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Respiratory Quinone ◽  
Peter The Great ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Major Respiratory Quinone

Three novel heterotrophic, Gram-negative, yellow-pigmented, aerobic, gliding, oxidase- and catalase-positive bacteria were isolated from algae collected in the Gulf of Peter the Great, Sea of Japan. 16S rRNA gene sequence analysis revealed that the strains studied represented members of the family Flavobacteriaceae and showed 93·5–93·8 % similarity with their closest relative, Psychroserpens burtonensis. The DNA G+C content of the strains was 34–37 mol%. The major respiratory quinone was MK-6. The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1, iso-C16 : 0-3OH and iso-C17 : 0-3OH. On the basis of their phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the newly described bacteria have been assigned to the new genus Winogradskyella gen. nov., as Winogradskyella thalassocola sp. nov. (type strain, KMM 3907T=KCTC 12221T=LMG 22492T=DSM 15363T), Winogradskyella epiphytica sp. nov. (type strain, KMM 3906T=KCTC 12220T=LMG 22491T=CCUG 47091T) and Winogradskyella eximia sp. nov. (type strain, KMM 3944T (=KCTC 12219T=LMG 22474T).

scholarly journals Tumebacillus ginsengisoli sp. nov., isolated from soil of a ginseng field

2011 ◽  
Vol 61 (7) ◽  
pp. 1715-1719 ◽  
Author(s):  
Sang-Hoon Baek ◽  
Yingshun Cui ◽  
Sun-Chang Kim ◽  
Chang-Hao Cui ◽  
Chengri Yin ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene

A Gram-reaction-positive, rod-shaped, spore-forming bacterium, designated Gsoil 1105T, was isolated from soil of a ginseng field in Pocheon Province in South Korea and characterized in order to determine its taxonomic position. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belongs to the order Bacillales, showing the highest level of sequence similarity with respect to Tumebacillus permanentifrigoris Eur1 9.5T (94.6 %). The phylogenetic distances from other described species with validly published names within the order Bacillales were greater than 9.0 %. Strain Gsoil 1105T had a genomic DNA G+C content of 55.6 mol% and menaquinone 7 (MK-7) as the major respiratory quinone. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 1105T represents a novel species of the genus Tumebacillus, for which the name Tumebacillus ginsengisoli sp. nov. is proposed. The type strain is Gsoil 1105T ( = KCTC 13942T  = DSM 18389T).

scholarly journals Bacillus panaciterrae sp. nov., isolated from soil of a ginseng field

2006 ◽  
Vol 56 (12) ◽  
pp. 2861-2866 ◽  
Author(s):  
Leonid N. Ten ◽  
Sang-Hoon Baek ◽  
Wan-Taek Im ◽  
Qing-Mei Liu ◽  
Zubair Aslam ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
Distinct Phylogenetic Lineage

A Gram-positive, non-motile, endospore-forming bacterium, designated Gsoil 1517T, was isolated from soil of a ginseng field in Pocheon Province (South Korea) and was characterized in order to determine its taxonomic position, using a polyphasic approach. It was found to rod-shaped and aerobic or facultatively anaerobic. It grew optimally at 30 °C and at pH 6.5–7.0. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 1517T forms a distinct phylogenetic lineage within the genus Bacillus, being related to Bacillus funiculus JCM 11201T (96.8 %). The strain showed less than 94.3 % sequence similarity with other Bacillus species. The G+C content of the genomic DNA was found to be 47.8 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were iso-C15 : 0 (42.4 %), anteiso-C15 : 0 (17.4 %), iso-C14 : 0 (9.7 %) and C16 : 0 (6.0 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 1517T represents a novel species of the genus Bacillus, for which the name Bacillus panaciterrae sp. nov. is proposed. The type strain is Gsoil 1517T (=KCTC 13929T=CCUG 52470T=LMG 23408T).

scholarly journals Algoriphagus jejuensis sp. nov., isolated from seawater

2012 ◽  
Vol 62 (2) ◽  
pp. 409-413 ◽  
Author(s):  
Dong-Heon Lee ◽  
Hyung-Yeel Kahng ◽  
Sun Bok Lee
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
High Sequence Similarity ◽  
Respiratory Quinone ◽  
Distinct Lineage ◽  
Major Respiratory Quinone

A Gram-negative, pink-pigmented, non-motile, strictly aerobic rod, designated CNU040T, was isolated from seawater from the coast of Jeju Island in Korea. The temperature, pH and NaCl ranges for growth were 4–30 °C, pH 5.5–10.0 and 0–5.0 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CNU040T belonged to a distinct lineage in the genus Algoriphagus and exhibited high sequence similarity with Algoriphagus terrigena DS-44T (98.3 %) and Algoriphagus alkaliphilus AC-74T (96.6 %) and lower sequence similarity (<96.0 %) with all other members of the genus Algoriphagus. DNA–DNA relatedness between strain CNU040T and A. terrigena KCTC 12545T was 44.5 %. The DNA G+C content of the isolate was 48.5 mol% and the major respiratory quinone was menaquinone-7. The major cellular fatty acids were iso-C15 : 0 (28.6 %) and summed feature 3 (consisting of iso-C15 : 0 2-OH and/or C16 : 1ω7c; 24.0 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown amino lipid, one unknown aminophospholipid and three unknown polar lipids. On the basis of phenotypic, phylogenetic and genotypic data, strain CNU040T represents a novel species within the genus Algoriphagus, for which the name Algoriphagus jejuensis sp. nov. is proposed. The type strain is CNU040T ( = KCTC 22647T  = JCM 16112T).

scholarly journals Sphingomonas lacus sp. nov., an astaxanthin-dideoxyglycoside-producing species isolated from soil near a pond

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2824-2830 ◽  
Author(s):  
Jin Ho Kim ◽  
Se Hyeuk Kim ◽  
Kyong Ho Kim ◽  
Pyung Cheon Lee
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Cellular Fatty Acids ◽  
Gram Staining ◽  
Gene Sequence Analysis ◽  
Taxonomic Studies ◽  
Ubiquinone 10

Taxonomic studies were performed on an astaxanthin-dideoxyglycoside-producing strain, designated PB304T, isolated from soil near a pond in Daejeon city, South Korea. Cells of strain PB304T were Gram-staining-negative, strictly aerobic, orange-coloured and motile, and occurred as single or paired short chains. PB304T did not contain bacteriochlorophyll a. 16S rRNA gene sequence analysis revealed that strain PB304T was closely related to ‘Sphingomonas humi’ KCTC 12341 (98.7 %), Sphingomonas kaistensis KCTC 12344T (97.9 %), Sphingomonas astaxanthinifaciens DSM 22298T (97.6 %) and Sphingomonas ginsengisoli KCTC 12630T (97.5 %). Analysis of pufLM gene sequences revealed strain PB304T to be closely related to ‘S. humi’ KCTC 12341 (88.1 %). The major cellular fatty acids were C16 : 0, summed feature 4 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c), and summed feature 7 (comprising C18 : 1ω7c/ω9t/ω12t). Ubiquinone 10 (Q-10) was the sole quinone identified, and the major pigment was astaxanthin dideoxyglycoside. The major polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The polyamine was spermidine. The DNA–DNA relatedness values of strain PB304T with respect to its closest phylogenetic neighbours were 57.1 % for ‘S. humi’ KCTC 12341, 51.2 % for Sphingomonas kaistensis KCTC 12334T, 50.6 % for Sphingomonas astaxanthinifaciens DSM 22298T and 50.2 % for Sphingomonas ginsengisoli KCTC 12630T. The DNA G+C content of strain PB304T was 66.6 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain PB304T is concluded to represent a novel species of the genus Sphingomonas, for which the name Sphingomonas lacus is proposed. The type strain is PB304T ( = KCTC 32458T = CECT 8383T).

scholarly journals Granulosicoccus coccoides sp. nov., isolated from leaves of seagrass (Zostera marina)

2010 ◽  
Vol 60 (4) ◽  
pp. 972-976 ◽  
Author(s):  
Valerie V. Kurilenko ◽  
Richard Christen ◽  
Natalia V. Zhukova ◽  
Nataliya I. Kalinovskaya ◽  
Valery V. Mikhailov ◽  
...  
Keyword(s):  
Zostera Marina ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Negative Bacterium ◽  
Rrna Gene Sequence ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
Bacterium Strain ◽  
Genotypic Characteristics

A non-pigmented, motile, Gram-negative bacterium, strain Z 271T, was isolated from the surface of leaves of the seagrass Zostera marina which was collected in Troitza Bay (Sea of Japan, Pacific Ocean). The new isolate grew between 5 °C and 28 °C and was slightly halophilic, tolerating environments containing up to 5 % (w/v) NaCl. Strain Z 271T was able to degrade Tweens 20, 40 and 80 and partially degrade gelatin, but was unable to degrade casein. Phosphatidylethanolamine (36.9 %) and phosphatidylglycerol (63.1 %) were the predominant phospholipids. The major fatty acids included C18 : 1 ω7c (43.7 %), C16 : 1 ω7c (31.1 %) and C16 : 0 (16.8 %). The main respiratory quinone was Q-8. The DNA–DNA relatedness value of strain Z 271T with Granulosicoccus antarcticus IMCC3135T was 35 %. The G+C content of the DNA of strain Z 271T was 60.2 mol%. On the basis of phenotypic and genotypic characteristics and 16S rRNA gene sequence analysis, strain Z 271T represents a novel species of the genus Granulosicoccus for which the name Granulosicoccus coccoides sp. nov. is proposed. The type strain is Z 271T (=KMM 6014T=CIP 109923T).

scholarly journals Devosia Sediminis sp. nov., Isolated from Fubterranean Sediment.

2021 ◽  
Author(s):  
Fu Ma ◽  
Da Zhi Zi ◽  
Wei Li ◽  
Zhao Xuan Wang ◽  
Jun Lu ◽  
...  
Keyword(s):  
Northeast China ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Respiratory Quinone ◽  
Cellular Fatty Acids ◽  
Optimum Ph ◽  
Major Respiratory Quinone ◽  
Ubiquinone 10

Abstract A novel Gram- negative, cream-colored, rod-shaped, aerobic, non-motile bacterium, designated MSA67T, was isolated from a subterranean sediment sample of the Mohe Basin in Northeast China. Strain MSA67T was detected to grow at 4–40°C (optimum, 28–30°C), pH 5.0–10.0 (optimum, pH 7.0) and in 0.0–8.0 % (w/v) NaCl (optimum, 2.0–3.0 %). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain MSA67T was a member of the genus Devosia, with the highest similarity with D. riboflavina IFO13584T (98.0 %) and D. chinhatensis IPL18T (97.0 %). The major cellular fatty acids are C16:0, C18:1ω7c 11-methyl and C18:1ω6c and/or C18:1ω7c. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, glycolipids and three unidentified phospholipids. The major respiratory quinone is ubiquinone 10 (Q-10). The genomic size of strain MSA67T is 4.1 MB and DNA G + C content is 63.6 %. Based on genotypic, phenotypic and phylogenetic results, strain MSA67T is concluded to represent a novel species of the genus Devosia, for which the name Devosia sediminis sp. nov. is proposed. The type strain is MSA67T (= CGMCC 1.18467T = KCTC 82192T).

scholarly journals Pandoraea thiooxydans sp. nov., a facultatively chemolithotrophic, thiosulfate-oxidizing bacterium isolated from rhizosphere soils of sesame (Sesamum indicum L.)

2010 ◽  
Vol 60 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Rangasamy Anandham ◽  
Pandiyan Indiragandhi ◽  
Soon Wo Kwon ◽  
Tong Min Sa ◽  
Che Ok Jeon ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Sesamum Indicum ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Respiratory Quinone ◽  
Rhizosphere Soils ◽  
Cellular Fatty Acids ◽  
Gene Sequence Analysis

A facultatively chemolithoautotrophic, thiosulfate-oxidizing, Gram-negative, aerobic, motile, rod-shaped bacterial strain, designated ATSB16T, was isolated from rhizosphere soils of sesame (Sesamum indicum L.). 16S rRNA gene sequence analysis demonstrated that this strain was closely related to Pandoraea pnomenusa LMG 18087T (96.7 % similarity), P. pulmonicola LMG 18016T (96.5 %), P. apista LMG 16407T (96.2 %), P. norimbergensis LMG 18379T (96.1 %) and P. sputorum LMG 18819T (96.0 %). Strain ATSB16T shared 96.0–96.4 % sequence similarity with four unnamed genomospecies of Pandoraea. The major cellular fatty acids of the strain ATSB16T were C17 : 0 cyclo (33.0 %) and C16 : 0 (30.6 %). Q-8 was the predominant respiratory quinone. The major polar lipids were phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified aminophospholipids. Hydroxyputrescine and putrescine were the predominant polyamines. The genomic DNA G+C content of the strain was 64.0 mol%. On the basis of the results obtained from this study, strain ATSB16T represents a novel species of the genus Pandoraea, for which the name Pandoraea thiooxydans sp. nov. is proposed. The type strain is ATSB16T (=KACC 12757T =LMG 24779T).

scholarly journals Jeotgalicoccus huakuii sp. nov., a halotolerant bacterium isolated from seaside soil

2010 ◽  
Vol 60 (6) ◽  
pp. 1307-1310 ◽  
Author(s):  
Xin-Qiang Guo ◽  
Rong Li ◽  
Liu-Qiang Zheng ◽  
Dong-Qing Lin ◽  
Ji-Quan Sun ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Fatty Acid Profiles ◽  
Gram Stain ◽  
Rrna Gene Sequence ◽  
Phenotypic Characteristics ◽  
Cellular Fatty Acids ◽  
Gene Sequence Analysis

A Gram-stain-positive, oxidase- and catalase-positive, non-motile, non-spore-forming, halotolerant, coccoid bacterium, designated strain NY-2T, was isolated from a seaside soil sample from Shandong Province, China. Strain NY-2T was able to grow in the presence of 0–23 % (w/v) NaCl and at pH 4.5–10.0 and 5–42 °C; optimum growth was observed with 3–8 % (w/v) total salts and at pH 6.5–8.0 and 28–37 °C. Chemotaxonomic analyses, including fatty acid profiles, menaquinones and polar lipids, supported the affiliation of strain NY-2T to the genus Jeotgalicoccus. The predominant menaquinone of strain NY-2T was menaquinone 7 (MK-7) (100 %) and the major cellular fatty acids were iso-C15 : 0 (49.0 %) and anteiso-C15 : 0 (19.6 %). Cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol and several unidentified phospholipids. The DNA G+C content of strain NY-2T was 36.8 mol%. Based on 16S rRNA gene sequence analysis, strain NY-2T formed a coherent cluster with Jeotgalicoccus marinus JSM 076033T, Jeotgalicoccus halotolerans YKJ-101T and Jeotgalicoccus psychrophilus YKJ-115T. Phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data indicated that strain NY-2T (=CCTCC AB 208288T =JCM 15687T) should be classified as the type strain of a novel species of the genus Jeotgalicoccus, for which the name Jeotgalicoccus huakuii sp. nov. is proposed.

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