Flavobacterium squillarum sp. nov., isolated from a freshwater shrimp culture pond, and emended descriptions of
Flavobacterium haoranii
,
Flavobacterium cauense
,
Flavobacterium terrae
and
Flavobacterium aquatile
A bacterial strain, designated CMJ-5T, was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-5T were Gram-stain-negative, strictly aerobic, non-motile, yellow-pigmented rods surrounded by a thick capsule. Growth occurred at 20–35 °C (optimum, 30 °C), at pH 6.5–8.0 (optimum, pH 7.0) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-5T belonged to the genus Flavobacterium and was related most closely to Flavobacterium haoranii LQY-7T with sequence similarity of 94.4 %. Strain CMJ-5T contained iso-C15 : 0 (37.5 %), iso-C17 : 0 3-OH (13.4 %), iso-C15 : 0 3-OH (13.1 %) and iso-C15 : 1 G (11.2 %) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The G+C content of the genomic DNA was 42.4 mol%. On the basis of phylogenetic inference and phenotypic data, strain CMJ-5T should be classified as a representative of a novel species, for which the name Flavobacterium squillarum sp. nov. is proposed. The type strain is CMJ-5T ( = BCRC 80405T = LMG 26890T = KCTC 23915T). Emended descriptions of Flavobacterium haoranii , Flavobacterium cauense , Flavobacterium terrae and Flavobacterium aquatile are also proposed.