Cyclobacterium caenipelagi sp. nov., isolated from a tidal flat sediment, and emended description of the genus Cyclobacterium

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3158-3163 ◽  
Author(s):  
Yong-Taek Jung ◽  
Jung-Sook Lee ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Tidal Flat Sediment ◽  
Emended Description

A Gram-staining-negative, aerobic, non-flagellated, non-gliding and ring-like or horseshoe-shaped bacterial strain, designated HD-17T, was isolated from a tidal flat sediment in the Korean peninsula. Strain HD-17T grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain HD-17T fell within the clade comprising species of the genus Cyclobacterium . Strain HD-17T exhibited 16S rRNA gene sequence similarity values of 93.8–98.8 % to the type strains of species of the genus Cyclobacterium . Strain HD-17T contained MK-7 as the predominant menaquinone and iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), anteiso-C15 : 0 and iso-C15 : 1 G as the major fatty acids. The major polar lipids of strain HD-17T were phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain HD-17T was 43.8 mol% and its DNA–DNA relatedness values with Cyclobacterium amurskyense KCTC 12363T, Cyclobacterium qasimii KCTC 23011T and Cyclobacterium marinum KCTC 2917T were 10.4, 7.6 and 5.3 %, respectively. The phylogenetic and genetic distinctiveness and several differentiating phenotypic properties revealed that strain HD-17T was separate from other species of the genus Cyclobacterium . On the basis of the data presented, strain HD-17T represents a novel species of the genus Cyclobacterium , for which the name Cyclobacterium caenipelagi sp. nov. is proposed. The type strain is HD-17T ( = KCTC 32178T = CCUG 63247T). An emended description of the genus Cyclobacterium is also provided.

Defluviimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat, and emended description of the genus Defluviimonas Foesel et al. 2011

2013 ◽  
Vol 63 (Pt_8) ◽  
pp. 2895-2900 ◽  
Author(s):  
Renukaradhya K. Math ◽  
Hyun Mi Jin ◽  
Sang Hyeon Jeong ◽  
Che Ok Jeon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Polar Lipid ◽  
Tidal Flat ◽  
Type Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A novel Gram-staining-negative, strictly aerobic bacterium, designated BS14T, was isolated from a marine tidal flat of the South Sea in Korea. Colonies were opaque, white, smooth and circular on marine agar. Cells were moderately halophilic, non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain BS14T was observed at 5–40 °C (optimum: 30 °C), pH 6.5–9.5 (optimum: 7.0–7.5) and 0–10 % (w/v) NaCl (optimum: 1–1.5 %). The G+C content of the genomic DNA was 61.6 mol%. Strain BS14T contained ubiquinone-10 (Q-10) as the sole respiratory quinone and summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C18 : 0 3-OH, C10 : 0 3-OH and C18 : 0 as the major fatty acids. The polar lipid pattern comprised phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminolipid, an unidentified phospholipid and an unidentified polar lipid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BS14T formed a tight phylogenetic lineage with Defluviimonas denitrificans D9-3T with a bootstrap value of 100 %. The 16S rRNA gene sequence similarity between strain BS14T and D. denitrificans D9-3T was 97.4 % and their DNA–DNA relatedness was 19.1±3.6 %. Based on the phenotypic and genotypic studies, strain BS14T represents a novel species of the genus Defluviimonas , for which the name Defluviimonas aestuarii sp. nov. is proposed. The type strain is BS14T ( = KACC 16442T = JCM 18630T). An emended description of the genus Defluviimonas Foesel et al. 2011 is also proposed.

Kangiella sediminilitoris sp. nov., isolated from a tidal flat sediment

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 1001-1006 ◽  
Author(s):  
Soo-Young Lee ◽  
Sooyeon Park ◽  
Tae-Kwang Oh ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Species ◽  
Phylogenetic Trees ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Tidal Flat Sediment

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped bacterial strain, BB-Mw22T, was isolated from a tidal flat sediment of the South Sea in South Korea. It grew optimally at 30–37 °C, at pH 7.0–7.5 and in the presence of 2–3 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain BB-Mw22T belonged to the genus Kangiella and the cluster comprising Kangiella species and strain BB-Mw22T was clearly separated from other taxa. Strain BB-Mw22T exhibited 95.3–98.7 % 16S rRNA gene sequence similarity to the type strains of recognized Kangiella species. Strain BB-Mw22T contained Q-8 as the predominant ubiquionone and iso-C15 : 0 and iso-C11 : 0 3-OH as the major fatty acids. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and one unidentified aminolipid. The DNA G+C content of strain BB-Mw22T was 48.9 mol%, and its mean DNA–DNA hybridization values with Kangiella geojedonensis YCS-5T, Kangiella japonica JCM 16211T and Kangiella taiwanensis JCM 17727T were 14–28 %. Phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain BB-Mw22T is distinguishable from all recognized Kangiella species. On the basis of the data presented, strain BB-Mw22T is considered to represent a novel species of the genus Kangiella , for which the name Kangiella sediminilitoris sp. nov. is proposed. The type strain is BB-Mw22T ( = KCTC 23892T  = CCUG 62217T).

scholarly journals Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 2969-2974 ◽  
Author(s):  
Sooyeon Park ◽  
Sung-Min Won ◽  
Hyangmi Kim ◽  
Doo-Sang Park ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Species ◽  
Phylogenetic Trees ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Tidal Flat Sediment

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, BS-B2T, which was isolated from a tidal flat sediment at Boseong in South Korea, was characterized taxonomically. Strain BS-B2T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The novel strain exhibited highest 16S rRNA gene sequence similarity (97.4 %) to Marivita geojedonensis DPG-138T. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain BS-B2T is closely related to Primorskyibacter sedentarius KMM 9018T, showing 96.5 % sequence similarity. Strain BS-B2T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the predominant fatty acid. The polar lipid profile of strain BS-B2T comprised phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid as major components, and differentiated it from the type strains of P. sedentarius and M. geojedonensis . The DNA G+C content of strain BS-B2T was 62.2 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain BS-B2T can be distinguished from phylogenetically related genera as well as P. sedentarius and M. geojedonensis . On the basis of the data presented, strain BS-B2T is considered to represent a novel species of a new genus, for which the name Aestuariivita boseongensis gen. nov., sp. nov. is proposed. The type strain of Aestuariivita boseongensis is BS-B2T ( = KCTC 42052T = CECT 8532T).

Algoriphagus chungangensis sp. nov., isolated from a tidal flat sediment

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 648-653 ◽  
Author(s):  
Hyeonji Kang ◽  
Veeraya Weerawongwiwat ◽  
Min Young Jung ◽  
Soon Chul Myung ◽  
Wonyong Kim
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
Tidal Flat Sediment

A Gram-stain-negative, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated CAU 1002T, was isolated from a tidal flat sediment and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1002T grew optimally at 30 °C and pH 7.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1002T formed a distinct lineage within the genus Algoriphagus and was most closely related to Algoriphagus lutimaris KCTC 22630T and Algoriphagus halophilus KCTC 12051T (97.75 and 97.74 % 16S rRNA gene sequence similarity, respectively). The strain contained MK-7 as the major isoprenoid quinone and iso-C15 : 0 and C16 : 1ω7c and/or iso-C15 : 0 2-OH (summed feature 3) as the major fatty acids. The cell-wall peptidoglycan of strain CAU 1002T contained meso-diaminopimelic acids. The major whole-cell sugars were glucose, arabinose, sucrose, and ribose. The polar lipid profile was composed of phosphatidylethanolamine, five unidentified aminolipids, one unidentified aminophospholipid, one unidentified phospholipid, one unidentified aminoglycolipid, one unidentified glycolipid and twelve unidentified lipids. The DNA G+C content of strain CAU 1002T was 38.0 mol%. On the basis of phylogenetic inference, phenotypic, chemotaxonomic and genotypic data, strain CAU 1002T should be classified into the genus Algoriphagus as a member of a novel species, for which the name Algoriphagus chungangensis sp. nov. is proposed. The type strain is CAU 1002T ( = KCTC 23759T = CCUG 61890T). The description of the genus Algoriphagus is emended.

scholarly journals Salinimicrobium gaetbulicola sp. nov., isolated from tidal flat sediment

2012 ◽  
Vol 62 (Pt_5) ◽  
pp. 1027-1031 ◽  
Author(s):  
Soo-Young Lee ◽  
Sooyeon Park ◽  
Tae-Kwang Oh ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Tidal Flat Sediment

A Gram-stain-negative, aerobic, non-flagellated, non-gliding and short rod- or rod-shaped bacterial strain, designated BB-My20T, was isolated from tidal flat sediment taken from the southern coast of Korea. Strain BB-My20T grew optimally at 37 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed that strain BB-My20T fell within the clade comprising Salinimicrobium species, joining Salinimicrobium catena HY1T, with which it had a 16S rRNA gene sequence similarity value of 97.4 %. It exhibited 95.4–96.9 % sequence similarity to the type strains of other members of the genus Salinimicrobium . Strain BB-My20T contained MK-6 as the predominant menaquinone and iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids detected in strain BB-My20T and S. catena JCM 14015T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain BB-My20T was 45.1 mol% and its mean DNA–DNA relatedness value with S. catena JCM 14015T was 4.5 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain BB-My20T can be distinguished from the four recognized species of the genus Salinimicrobium . On the basis of the data presented, strain BB-My20T is considered to represent a novel species of the genus Salinimicrobium , for which the name Salinimicrobium gaetbulicola sp. nov. is proposed; the type strain is BB-My20T ( = KCTC 23579T = CCUG 60898T).

scholarly journals Winogradskyella wandonensis sp. nov., isolated from a tidal flat

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1520-1525 ◽  
Author(s):  
Sooyeon Park ◽  
Ji-Min Park ◽  
Sung-Min Won ◽  
Kyung Sook Bae ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Strain ◽  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type

A Gram-stain-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated WD-2-2T, was isolated from a tidal flat of Wando, an island of South Korea, and subjected to a polyphasic taxonomic analysis. Strain WD-2-2T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WD-2-2T belonged to the genus Winogradskyella , clustering coherently with the type strain of Winogradskyella litorisediminis . Strain WD-2-2T exhibited 16S rRNA gene sequence similarity of 97.4 % to W. litorisediminis DPS-8T and 94.5–96.6 % to the type strains of the other species of the genus Winogradskyella . Strain WD-2-2T contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C17 : 0 3-OH and iso-C15 : 0 as the major fatty acids. The major polar lipids detected in strain WD-2-2T were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content was 36.4 mol%, and DNA–DNA relatedness with W. litorisediminis DPS-8T was 13 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain WD-2-2T is separate from recognized species of the genus Winogradskyella . On the basis of the data presented, strain WD-2-2T is considered to represent a novel species of the genus Winogradskyella , for which the name Winogradskyella wandonensis sp. nov. is proposed. The type strain is WD-2-2T ( = KCTC 32579T = CECT 8445T).

scholarly journals Granulosicoccus marinus sp. nov., isolated from Antarctic seawater, and emended description of the genus Granulosicoccus

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4103-4108 ◽  
Author(s):  
Kiwoon Baek ◽  
Ahyoung Choi ◽  
Ilnam Kang ◽  
Mihye Im ◽  
Jang-Cheon Cho
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Dna Relatedness ◽  
Link Type ◽  
Emended Description

A Gram-staining-negative, motile by flagella, non-pigmented, poly-β-hydroxybutyrate-producing, strictly aerobic and sphere-shaped bacterium, IMCC3490T, was isolated from a coastal seawater sample from the Antarctic Peninsula. Optimal growth of strain IMCC3490T was observed at 20 °C, pH 8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain IMCC3490T belonged to the genus Granulosicoccus in the family Granulosicoccaceae . The strain was closely related to Granulosicoccus antarcticus IMCC3135T (98.8 % 16S rRNA gene sequence similarity) and Granulosicoccus coccoides Z 271T (98.5 %). The DNA–DNA relatedness values between IMCC3490T and type strains of the two species of the genus were far lower than 70 %, which indicated strain IMCC3490T is a novel genomic species of the genus Granulosicoccus . The major fatty acids of strain IMCC3490T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The isoprenoid quinone detected was ubiquinone-8 (Q-8) and predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 61.0 mol%. On the basis of phylogenetic analyses, DNA–DNA relatedness values and phenotypic data, it is suggested that strain IMCC3490T represents a novel species of the genus Granulosicoccus , for which the name Granulosicoccus marinus sp. nov. is proposed. The type strain is IMCC3490T ( = KACC 17483T = NBRC 109704T). An emended description of the genus Granulosicoccus is also provided.

Olleya namhaensis sp. nov., isolated from wood falls, and emended description of the genus Olleya Mancuso Nichols et al. 2005 emend. Lee et al. 2010

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1610-1615 ◽  
Author(s):  
Mi-Hwa Lee ◽  
Yong-Taek Jung ◽  
Sooyeon Park ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A Gram-stain-negative, non-flagellated, gliding, rod-shaped bacterial strain, designated WT-MY15T, was isolated from wood falls in the South Sea in Korea and subjected to a polyphasic taxonomic study. Strain WT-MY15T grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WT-MY15T clustered with the type strains of two Olleya species, exhibiting 16S rRNA gene sequence similarity values of 97.7–98.1 %. Strain WT-MY15T contained MK-6 as the predominant menaquinone. The fatty acid and polar lipid profiles of strain WT-MY15T were similar to those of Olleya aquimaris L-4T and Olleya marilimosa CIP 108537T. The DNA G+C content of strain WT-MY15T was 42.8 mol% and its mean DNA–DNA relatedness values with O. aquimaris L-4T and O. marilimosa CIP 108537T were 8.3 and 5.6 %, respectively. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WT-MY15T is separate from the two recognized species of the genus Olleya . On the basis of the data presented, strain WT-MY15T is considered to represent a novel species of the genus Olleya , for which the name Olleya namhaensis sp. nov. is proposed. The type strain is WT-MY15T ( = KCTC 23673T = CCUG 61507T). An emended description of the genus Olleya is also presented.

scholarly journals Erythrobacter lutimaris sp. nov., isolated from a tidal flat sediment

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4184-4190 ◽  
Author(s):  
Yong-Taek Jung ◽  
Sooyeon Park ◽  
Jung-Sook Lee ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Species ◽  
Sequence Similarity ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Tidal Flat Sediment

A Gram-stain-negative, non-motile, coccoid- or oval-shaped bacterial strain, designated S-5T, belonging to the class Alphaproteobacteria , was isolated from a tidal flat sediment of the Yellow Sea, Korea and was subjected to a polyphasic taxonomic study. Strain S-5T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. Neighbour-joining analysis based on 16S rRNA gene sequences showed that strain S-5T fell within the clade comprising the species of the genus Erythrobacter , clustering with the type strains of Erythrobacter pelagi , Erythrobacter citreus and Erythrobacter seohaensis with which it exhibited the highest 16S rRNA gene sequence similarity (96.0–96.7 %). The DNA G+C content was 66.0 mol%. Strain S-5T contained Q-10 as the predominant ubiquinone and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C17 : 1ω6c as the major fatty acids. The major polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, an unidentified glycolipid and two unidentified lipids. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain S-5T is distinguishable from other species of the genus Erythrobacter . On the basis of the data presented, strain S-5T is considered to represent a novel species of the genus Erythrobacter , for which the name Erythrobacter lutimaris sp. nov. is proposed. The type strain is S-5T ( = KCTC 42109T = CECT 8624T).

Kordiimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat

2012 ◽  
Vol 62 (Pt_12) ◽  
pp. 3049-3054 ◽  
Author(s):  
Renukaradhya K. Math ◽  
Sang Hyeon Jeong ◽  
Hyun Mi Jin ◽  
Moon Su Park ◽  
Jeong Myeong Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Link Type

A Gram-staining negative, strictly aerobic bacterium, designated 101-1T, was isolated from a sea tidal flat, Taean, Korea. The strain formed small light-yellow, smooth, and circular colonies on marine agar. Cells were weakly halophilic, motile rods showing catalase- and oxidase-positive reactions. Growth of strain 101-1T was observed at 15–40 °C (optimum, 30 °C), pH 5.0–8.0 (optimum, pH 6.5–7.0) and 1.0–9.0 % (w/v) NaCl (optimum, 2.0–3.5 %). The G+C content of the genomic DNA was 53.3 mol%. Strain 101-1T contained ubiquinone-10 (Q-10) as the respiratory quinone and iso-C17 : 1ω9c, iso-C15 : 0 and iso-C17 : 0 as major fatty acids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 101-1T formed a tight phylogenetic lineage with members of the genus Kordiimonas and was most closely related to Kordiimonas gwangyangensis GW14-5T and Kordiimonas lacus S3-22T with 97.3 % and 96.3 % 16S rRNA gene sequence similarities, respectively. The DNA–DNA relatedness values between strain 101-1T and K. gwangyangensis GW14-5T and K. lacus S3-22T were 24.8±4.4 % and 32.2±3.6 %, respectively. Based on the data from the phenotypic and genotypic studies, strain 101-1T represents a novel species of the genus Kordiimonas , for which the name Kordiimonas aestuarii sp. nov. is proposed. The type strain is 101-1T ( = KACC 16184T = JCM 17742T).

Sign in / Sign up

Export Citation Format

Share Document