scholarly journals Salinigranum rubrum gen. nov., sp. nov., a member of the family Halobacteriaceae isolated from a marine solar saltern

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 2029-2033 ◽  
Author(s):  
Heng-Lin Cui ◽  
Wen-Jiao Zhang
Keyword(s):  
New Genus ◽  
Novel Species ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Optimum Ph ◽  
Pleomorphic Cells

Halophilic archaeal strain GX10T was isolated from the Gangxi marine solar saltern in China. Strain GX10T was observed to have pleomorphic cells that lysed in distilled water, stained Gram-negative and produced red-pigmented colonies. Strain GX10T was able to grow at 20–50 °C (optimum 37 °C), with 1.4–4.8 M NaCl (optimum 3.1 M NaCl), with 0–0.7 M MgCl2 (optimum 0.05 M MgCl2) and at pH 5.0–9.0 (optimum pH 7.0). The major polar lipids of strain GX10T were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, and five unidentified glycolipids. Phylogenetic tree reconstructions based on 16S rRNA gene and rpoB′ sequences revealed that strain GX10T was distinct from the related genera, Halogranum , Haloferax , Halopelagius , Halogeometricum , Halobellus , Haloplanus and Halorubrum . The DNA G+C content of strain GX10T was 62.9 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain GX10T represents a novel species of a new genus within the family Halobacteriaceae , for which the name Salinigranum rubrum gen. nov., sp. nov. is proposed. The type strain of the type species is GX10T ( = CGMCC 1.10385T = JCM 17116T).

Halobellus inordinatus sp. nov., from a marine solar saltern and an inland salt lake of China

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 3975-3980 ◽  
Author(s):  
Xing-Xing Qiu ◽  
Yun-Zhuang Mou ◽  
Mei-Lin Zhao ◽  
Wen-Jiao Zhang ◽  
Dong Han ◽  
...  
Keyword(s):  
Salt Lake ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Content Type ◽  
Optimum Growth Temperature ◽  
Link Type ◽  
The Family ◽  
The 16S Rrna Gene

Two halophilic archaeal strains, YC20T and XD15, were isolated from a marine solar saltern and an inland salt lake in China. Both had pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They were neutrophilic, requiring at least 100 g NaCl l−1 and 0.5–95 g MgCl2 l−1 for growth at the optimum growth temperature of 37 °C. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified glycolipids were also detected. The 16S rRNA gene sequences of the two strains were 99.5 % identical and showed 94.0–95.9 % similarity to the most closely related members of the genus Halobellus of the family Halobacteriaceae . The rpoB′ gene sequence similarity between strains YC20T and XD15 was 98.2 % and these sequences showed 89.6–92.8 % similarity to those of the most closely related members of the genus Halobellus . The DNA G+C contents of strains YC20T and XD15 were 65.8 mol% and 65.4 mol%, respectively. The DNA–DNA hybridization value between strain YC20T and strain XD15 was 92 %, and the two strains showed low DNA–DNA relatedness to members of the genus Halobellus . The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains YC20T and XD15 represent a novel species of the genus Halobellus , for which the name Halobellus inordinatus sp. nov. is proposed. The type strain is YC20T ( = CGMCC 1.12120T = JCM 18361T) and the other strain is XD15 ( = CGMCC 1.12236 = JCM 18648).

scholarly journals Puniceibacterium antarcticum gen. nov., sp. nov., isolated from seawater

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1566-1572 ◽  
Author(s):  
Chang Liu ◽  
Xi-Ying Zhang ◽  
Hai-Nan Su ◽  
Ming-Yang Zhou ◽  
Bo Chen ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Novel Species ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-reaction-negative, aerobic, non-flagellated, rod-shaped bacterium, designated strain SM1211T, was isolated from Antarctic seawater. The isolate grew at 4–35 °C and with 0–10 % (w/v) NaCl. It could produce bacteriochlorophyll a, but did not reduce nitrate to nitrite or hydrolyse DNA. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain SM1211T constituted a distinct phylogenetic line within the family Rhodobacteraceae and was closely related to species in the genera Litorimicrobium , Leisingera , Seohaeicola and Phaeobacter with 95.1–96.0 % similarities. The predominant cellular fatty acid was C18 : 1ω7c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and two unidentified phospholipids. The genomic DNA G+C content of strain SM1211T was 60.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain SM1211T is considered to represent a novel species in a new genus within the family Rhodobacteraceae , for which the name Puniceibacterium antarcticum gen. nov., sp. nov. is proposed. The type strain of Puniceibacterium antarcticum is SM1211T ( = CCTCC AB 2013147T = KACC 16875T).

Extensimonas vulgaris gen. nov., sp. nov., a member of the family Comamonadaceae

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2062-2068 ◽  
Author(s):  
Wei-Yan Zhang ◽  
Ming-Xu Fang ◽  
Wen-Wu Zhang ◽  
Chuan Xiao ◽  
Xin-Qi Zhang ◽  
...  
Keyword(s):  
Industrial Wastewater ◽  
New Genus ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Sequences Alignment

A novel strain, named S4T, was obtained from industrial wastewater in Xiaoshan, Zhejiang Province, China. Cells were Gram-negative, neutrophilic and non-spore-forming and moved by means of a polar flagellum. Normal cells were 0.8–0.9×1.3–1.9 µm and the cells elongated to 10–25 µm when cultivated at high temperatures. Strain S4T grew at 15–50 °C (optimum at 48 °C), pH 5.5–8.5 (optimum 7.0–7.5) and 0–2 % (optimum 0.5 %) (w/v) NaCl. Ubiquinone-8 was the predominant respiratory quinone. C16 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C17 : 0 cyclo were the major cellular fatty acids. The major 3-OH fatty acid was C10 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminoglycolipid. The genomic DNA G+C content was 68.8 mol%. Based on 16S rRNA gene sequences alignment, the most closely related strains were members of the genera Comamonas (94.6–95.6 % similarities), Giesbergeria (94.9–95.6 %), Acidovorax (94.8–95.4 %), Brachymonas (94.1–95.2 %) and Macromonas (95.1 %). Phylogenetic analysis showed the closest relatives of strain S4T were members of the genus Macromonas . Based on phenotypic and phylogenetic characteristics, we suggest that strain S4T represents a novel species of a new genus of the family Comamonadaceae , for which the name Extensimonas vulgaris gen. nov., sp. nov. is proposed. The type strain of Extensimonas vulgaris is S4T ( = CGMCC 1.10977T = JCM 17803T).

scholarly journals Halolamina pelagica gen. nov., sp. nov., a new member of the family Halobacteriaceae

2011 ◽  
Vol 61 (7) ◽  
pp. 1617-1621 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xia Gao ◽  
Xin Yang ◽  
Xue-Wei Xu
Keyword(s):  
Dna Hybridization ◽  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Marine Solar Saltern ◽  
The Family ◽  
Nacl Concentration ◽  
Optimum Ph

Two extremely halophilic archaeal strains, TBN21T and TBN49, were isolated from the Taibei marine solar saltern near Lianyungang city, Jiangsu province, China. Cells of the two strains were pleomorphic and Gram-negative and colonies were red. Strains TBN21T and TBN49 were able to grow at 25–50 °C (optimum 37 °C), at 1.4–5.1 M NaCl (optimum 3.4–3.9 M) and at pH 5.5–9.5 (optimum pH 7.0–7.5) and neither strain required Mg2+ for growth. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of the two strains were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and eight glycolipids; three of these glycolipids (GL3, GL4 and GL5) were chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1), galactosyl mannosyl glucosyl diether (TGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Phylogenetic analysis revealed that strains TBN21T and TBN49 formed a distinct clade with their closest relative, Halobaculum gomorrense JCM 9908T (89.0–89.5 % 16S rRNA gene sequence similarity). The DNA G+C contents of strains TBN21T and TBN49 were 64.8 and 62.7 mol%, respectively. DNA–DNA hybridization between strains TBN21T and TBN49 was 90.1 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains TBN21T and TBN49 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halolamina pelagica gen. nov., sp. nov. is proposed. The type strain of Halolamina pelagica is TBN21T ( = CGMCC 1.10329T  = JCM 16809T).

Wenyingzhuangia marina gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a recirculating mariculture system

2014 ◽  
Vol 64 (Pt_2) ◽  
pp. 469-474 ◽  
Author(s):  
Ying Liu ◽  
Liang-Zi Liu ◽  
Hong-Can Liu ◽  
Yu-Guang Zhou ◽  
Fang-Jun Qi ◽  
...  
Keyword(s):  
Type Species ◽  
New Genus ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Optimum Ph

A Gram-stain-negative, strictly aerobic and heterotrophic bacterial strain, designed strain D1T, was isolated from a recirculating mariculture system in Tianjin, China. Its taxonomic position was determined using a polyphasic approach. Cells of strain D1T were non-flagellated short rods, 0.3–0.5 µm wide and 0.5–1.0 µm long. Growth was observed at 15–30 °C (optimum, 25 °C), at pH 5.5–9.0 (optimum, pH 6.5–7.0) and in the presence of 1–8 % (w/v) NaCl (optimum, 2–3 %). Cells contained carotenoid pigments but not flexirubin-type pigments. Strain D1T contained MK-6 as the sole menaquinone and phosphatidylethanolamine (PE) as the sole phospholipid and four unidentified lipids. The major cellular fatty acids (>10 %) were iso-C15 : 0 (23.2 %), iso-C17 : 0 3-OH (15.2 %), C16 : 1ω7c/C16 : 1ω6c (14.3 %), iso-C15 : 0 3-OH (13.5 %) and iso-C15 : 1 G (10.8 %). 16S rRNA gene sequence analyses indicated that strain D1T belonged to the family Flavobacteriaceae and showed closest phylogenetic relationship to the genus Lutibacter , with highest sequence similarity to Lutibacter aestuarii MA-My1T (92.2 %). The DNA G+C content of strain D1T was 35.9 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain D1T was considered to represent a novel species in a new genus of the family Flavobacteriaceae , for which the name Wenyingzhuangia marina gen. nov., sp. nov. is proposed. The type strain of the type species is D1T ( = CGMCC 1.12162T = JCM 18494T).

Rudaibacter terrae gen. nov., sp. nov., isolated from greenhouse soil

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4052-4057 ◽  
Author(s):  
Soo-Jin Kim ◽  
Ji-Young Moon ◽  
Moriyuki Hamada ◽  
Tomohiko Tamura ◽  
Hang-Yeon Weon ◽  
...  
Keyword(s):  
Type Species ◽  
New Genus ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Greenhouse Soil ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Optimum Ph ◽  
Sequence Similarities

A novel Gram-stain-positive, non-motile, rod-shaped bacterium, designated 5GHs34-4T, was isolated from greenhouse soil in Yongin, Republic of Korea. Growth occurred in the temperature range of 10–37 °C (optimum 28–30 °C) and at pH 5.0–9.0 (optimum pH 7.0). It can tolerate up to 3 % (w/v) NaCl. The strain showed 16S rRNA gene sequence similarity levels of 95.1–97.0 % with species of the genus Leifsonia , 95.7–96.7 % with species of the genus Herbiconiux , 95.1–96.4 % with species of the genus Salinibacterium and 96.1 % with Labedella gwakjiensis and Homoserinimonas aerilata . The highest sequence similarities (97.0 %) were with Leifsonia aquatica JCM 1368T, Leifsonia poae VKM Ac-1401T and Leifsonia psychrotolerans LI1T. The peptidoglycan type determined for strain 5GHs34-4T was B2γ with dl-2,4-diaminobutyric acid at position 3. The murein was of the acetyl type. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The menaquinones detected were MK-13, MK-12 and MK-14, and the major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), anteiso-C17 : 0 and anteiso-C15 : 0. The phenotypic and phylogenetic traits of strain 5GHs34-4T differed in some respects from those of members of the family Microbacteriaceae . Therefore, strain 5GHs34-4T is considered to represent a novel species of a new genus in the family Microbacteriaceae , for which the name Rudaibacter terrae gen. nov., sp. nov. is proposed. The type strain is 5GHs34-4T ( = KACC 15523T = NBRC 108754T).

Fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 458-463 ◽  
Author(s):  
Sonia R. Vartoukian ◽  
Julia Downes ◽  
Richard M. Palmer ◽  
William G. Wade
Keyword(s):  
New Genus ◽  
Novel Species ◽  
Fusobacterium Nucleatum ◽  
Oral Bacteria ◽  
Rrna Gene ◽  
Good Growth ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Peptone Yeast Extract Glucose

SGP1T, a strain belonging to a lineage of the phylum Synergistetes with no previously cultivated representatives was subjected to a comprehensive range of phenotypic and genotypic tests. For good growth the strain was dependent on co-culture with, or extracts from, selected other oral bacteria. Cells of strain SGP1T were asaccharolytic and major amounts of acetic acid and moderate amounts of propionic acid were produced as end products of metabolism in peptone-yeast extract-glucose broth supplemented with a filtered cell sonicate of Fusobacterium nucleatum subsp. nucleatum ATCC 25586T (25 %, v/v). Hydrogen sulphide was produced and gelatin was weakly hydrolysed. The major cellular fatty acids were C14 : 0, C18 : 0 and C16 : 0. The DNA G+C content of strain SGP1T was 63 mol%. Phylogenetic analysis of the full-length 16S rRNA gene showed that strain SGP1T represented a novel group within the phylum Synergistetes . A novel species in a new genus, Fretibacterium fastidiosum gen. nov., sp. nov., is proposed. The type strain of Fretibacterium fastidiosum is SGP1T ( = DSM 25557T = JCM 16858T).

Muriicola soli sp. nov., isolated from soil

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
Hye Jeong Kang ◽  
Min-Kyeong Kim ◽  
Su Gwon Roh ◽  
Seung Bum Kim
Keyword(s):  
Related Species ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Optimum Ph ◽  
Genomic Characterization

A Gram-stain-negative, oxidase-positive, catalase-positive, aerobic, orange-pigmented, rod-shaped and non-motile bacterium designated strain MMS17-SY002T was isolated from island soil. The isolate grew at 20–37 °C (optimum, 30 °C), at pH 6.0–9.5 (optimum, pH 7) and in the presence of 0.5–4.0 % (w/v) NaCl (optimum, 2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MMS17-SY002T was mostly related to the genus Muriicola of the family Flavobacteriaceae and had highest sequence similarity of 96.82 % to Muriicola marianensis A6B8T and Muriicola jejuensis EM44T, but formed a distinct phylogenetic line within the genus. Chemotaxonomic analyses showed that menaquinone 6 was the predominant isoprenoid quinone, the major fatty acids were iso-C15 : 1 G and iso-C15 : 0, and the diagnostic polar lipid was phosphatidylethanolamine. The genomic DNA G+C content was 42.4 mol%. Strain MMS17-SY002T could be distinguished from related species by the combination of trypsin, α-chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, α-galactosidase, β-galactosidase and β-glucosidase activities. The orthologous average nucleotide identity between the genomes of strain MMS17-SY002T and M. jejuensis and that between the strain and M. marianensis A6B8T were 73.26 and 73.33%, respectively, thus confirming the separation of the strain from related species at species level. Based on the phenotypic, phylogenetic, chemotaxonomic and genomic characterization, MMS17-SY002T should be recognized as a novel species of the genus Muriicola , for which the name Muriicola soli sp. nov. is proposed. The type strain is MMS17-SY002T (=KCTC 62790T=JCM 32370T).

scholarly journals Mesoflavibacter aestuarii sp. nov., a zeaxanthin-producing marine bacterium isolated from seawater

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 1932-1937 ◽  
Author(s):  
Ji Hee Lee ◽  
Yeoung Min Hwang ◽  
Keun Sik Baik ◽  
Kap Seong Choi ◽  
Jong-Ok Ka ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Carotenoid Pigment ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Gwangyang Bay

An orange, rod-shaped, Gram-reaction-negative, aerobic and gliding bacterial strain devoid of flagella, designated strain KYW614T, was isolated from seawater collected from Gwangyang Bay, Republic of Korea. Zeaxanthin was the major carotenoid pigment produced and flexirubin-type pigments were not produced. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KYW614T belonged to the family Flavobacteriaceae and it was most closely related to Mesoflavibacter zeaxanthinifaciens TD-ZX30T (96.5 %, sequence similarity). The predominant cellular fatty acids of strain KYW614T were iso-C15 : 1 G (10.5 %), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c; 10.0 %), iso-C15 : 0 (9.5 %), C15 : 0 (7.5 %) and iso-C17 : 0 3-OH (7.4 %). MK-6 was the only isoprenoid quinone and the DNA G+C content was 32.6 mol%. Data from a polyphasic taxonomic study suggested that the isolate represents a novel species in the genus Mesoflavibacter , for which the name Mesoflavibacter aestuarii sp. nov. is proposed. The type strain is KYW614T ( = KCTC 32269T = JCM 19524T).

scholarly journals Sphingobacterium paludis sp. nov., isolated from wetland soil

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3453-3458 ◽  
Author(s):  
Hao Feng ◽  
Yanhua Zeng ◽  
Yili Huang
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Wetland Soil ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Optimum Ph ◽  
Xixi Wetland

A novel Gram-stain-negative bacteria, designated S37T, was isolated from soil of the Xixi wetland, Zhejiang province, China. Cells of strain S37T were aerobic, non-motile rods. Growth occurred at 10–37 °C (optimum, 25 °C), pH 5.0–9.7 (optimum, pH 7.5) and with 0–6 % (w/v) NaCl (optimum, 0.5 %). Based on 16S rRNA gene sequence analysis, strain S37T was found to be a member of the genus Sphingobacterium and shared highest similarity with Sphingobacterium composti 4M24T (95.78 %). The major fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 0 and iso-C17 : 0 3-OH, and the DNA G+C content was 43.8 mol%. The predominant respiratory quinone was MK-7. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain S37T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium paludis sp. nov. (type strain S37T = CGMCC 1.12801T = NBRC 110386T) is proposed.

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