Pseudomonas Tumuqiensis Sp. Nov., Isolated From Greenhouse Soil

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, named LAMW06T, was isolated from greenhouse soil in Beijing, China. In the 16S rRNA gene sequence comparison, strain LAMW06T had the highest similarity with Pseudomonas cuatrocienegasensis 1NT was 97.4%. Phylogenetic analysis base on the 16S rRNA and three housekeeping gene sequences (gyrB, rpoB and rpoD) indicated that LAMW06T represented a member of the genus Pseudomonas. The genome sequence size of the isolate was 5.5 Mb, with the DNA G+C content of 63.5 mol%. The average nucleotide identity and DNA–DNA hybridization values between strain LAMW06T and closely related members of Pseudomonas borbori R-20821T, Pseudomonas taeanensis MS-3T and Pseudomonas cuatrocienegasensis 1NT were 90.9%, 82.4%, 81.5 % and 43.0%, 25.9%, 24.6 % respectively. The major fatty acids contained summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C18:1 ω7c and C16:0. The primary respiratory quinone was ubiquinone-9. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, six aminophospholipids, six phospholipids, one aminolipid and one glycolipid. According to the genotypic, phylogenetic and chemotaxonomic data, strain LAMW06T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas tumuqiensis sp. nov. is proposed. The type strain is LAMW06T (=GDMCC 1.2003T =KCTC 72829T).

Efficiency of Reductive Soil Disinfestation Affected by Soil Water Content and Organic Amendment Rate

Reductive Soil Disinfestation (RSD) is a good method which can restore degraded greenhouse soil and effectively inactivate soil-borne pathogens. However, the approach needs to be optimized in order to facilitate its practical application in various regions. In the present work, we investigated the effect of soil water content (60% water holding capacity (WHC), 100% WHC and continuous flooding) and maize straw application rates (0, 5, 10, and 20 g kg−1 soil) on the improvement of soil properties and suppression of soil-borne pathogens (Fusarium oxysporum, Pythium and Phytophthora). The results showed that increasing the soil water content and maize straw application rate accelerated the removal of excess sulfate and nitrate in the soil and elevated the soil pH. Elevating the water content and maize straw application rate also produced much more organic acids, which could strongly inhibit soil-borne pathogens. Soil properties were improved significantly after RSD treatment with a maize straw amendment rate of more than 5 g kg−1, regardless of the water content. However, RSD treatments with 60% WHC could not effectively inactivate soil-borne pathogens and even stimulated their growth by increasing the maize application rate. RSD treatments of both 100% WHC and continuous flooding could inactivate soil-borne pathogens and increase the pathogens mortality indicated by cultural cells relatively effectively. The inhibited pathogens were significantly increased with the increasing maize application rate from 5 g kg−1 to 10 g kg−1, but were not further increased from 10 g kg−1 to 20 g kg−1. A further increased mortality of F. oxysporum, indicated by gene copies, was also observed when the soil water content and maize straw application rate were increased. Therefore, RSD treatment with 60% WHC could improve soil properties significantly, whereas irrigation with 100% WHC or continuous flooding was a necessity for effective soil-borne pathogens suppression. Holding 100% WHC and applicating maize straw at 10 g kg−1 soil were optimum conditions for RSD field operation to restore degraded greenhouse soil.

PERANCANGAN DAN PENGUJIAN ALAT UNTUK MONITORING KELEMBABAN TANAH DAN PEMBERIAN PUPUK CAIR PADA TANAMAN CABAI BERBASIS INTERNET Of THINGS

ABSTRAKTanah sebagai faktor utama dalam pertanian harus dipertimbangkan sebaik mungkin agar dapat memberikan hasil seperti yang diharapkan. Salah satunya dengan memanfaatkan teknologi komputer dan internet untuk memonitor kelembaban tanah. Kelembaban tanah adalah salah satu faktor lingkungan yang mempengaruhi pertumbuhan tanaman. Salah satu inovasi teknologi informasi dan komunikasi di bidang pertanian adalah penggunaan Internet of Things. Dengan menggunakan Internet Of Things, hal itu bisa dilakukan untuk memantau kelembaban tanah yang menjadi media tanam tanaman hortikultura. mengetahui nilai kelembaban tanah akan sangat berguna untuk dapat menentukan langkah atau penanganan tanah. Masalah utama adalah menurun nya tingkat kesuburan tanah yang berpengaruh terhadap menurun nya produktifitas hasil panen cabe. Dengan alat yang dibuat nantinya, tingkat kesuburan tanah akan terjaga.Kata kunci: ESP32,Greenhouse, Soil Moiusture, Internet of Things

Woody and semi-woody species in the collection “Rare and endangered plant species of Siberia” (Novosibirsk)

. The article presents the results of the conservation of woody and semi-woody species under introductionconditions in the collection “Rare and endangered plant species of Siberia” of the Central Siberian Botanical Garden (Novosibirsk). This collection was founded in 1965 years. The analysis of the fund of this collection is carried out. For a longtime (more than 10 years), 22 woody and semi-woody species from 14 families from 8 regions of Siberia are grown in thecollection. Four species are endemic: Sedum populifolium Pallas, Cotoneaster lucidus Schlecht., Sibiraea altaiensis (Laxm.)Schneider and Tilia sibirica Fisher ex Bayer. Most of the species are represented in the collection by small populations. Thisis due to the problems of reproduction of species in the conditions of culture, for many of which methods of vegetative andseed reproduction have not been developed. The traditional method of reproduction used for herbaceous plants – laboratory-greenhouse-soil. This method is often ineffective for woody species due to the long pregenerative period and low biological longevity of seeds. In the introduction, microecological conditions are selected for each species, since they belongto different ecological groups. Among the introduced species there are 13 mesophytes, 8 xerophytes, 1 xeromesophyte. Thearticle presents literary and original information about the methods of reproduction of cultivated species.

Nutrients Analysis of Greenhouse Soil Nursed with the Organic Fertilizers

The present work based on soil nutrients analysis of covered and uncovered fields nursed with organic fertilizer and cultivated with various crops. Collected the soil from three deep bottoms (5 cm, 15 cm, and 30 cm), mixed, and prepared five different samples. Calculated the pH value, electrical conductivity, and the percentage of nitrogen, phosphorus, Potassium, the humus of the collected soil samples and documented all the parameters. The results showed that the soil has moderately high pH values (7.9 in the greenhouse and 7.5 in the uncovered area), the E.C of greenhouse (4.1dS/m) is slightly lower than the uncovered area (4.8dS/m), nitrogen (6.3% than in the uncovered field 4.7%), relatively high concentration of phosphorus (5.16 mg/100g in the greenhouse uncovered field 4.67 mg/g), relatively high potassium values (39.04mg/100g in the greenhouse and 36.30 mg/g in the uncovered area) and high values of humus (12.7 % in the greenhouse and 9.1 % in the uncovered area). Concluded that the soil is saturated in the greenhouse with phosphorus and Potassium, no need to further fertilizations in the forthcoming years, and thegreenhouse is better for crop cultivation.

Productive Characteristics and Fruit Quality Traits of Cherry Tomato Hybrids as Modulated by Grafting on Different Solanum spp. Rootstocks under Ralstonia solanacearum Infested Greenhouse Soil

Grafting is increasingly becoming an indispensable tool that minimizes the risks associated with intensive vegetable production systems, including soil-borne diseases. This study assesses the performance of two cherry tomato hybrids (‘Cheramy’ and ‘Sheeja’) grafted onto three tomato and five eggplant local rootstock genotypes (cultivated/wild) under Ralstonia solanacearum (bacterial wilt)-infested greenhouse soil. The impact of grafting on growth, yield and fruit physical quality was mainly influenced by the response of rootstocks to disease resistance. The non-grafted plants of both the cultivars were severely affected by bacterial wilt, thus presenting high susceptibility to disease. Eggplant rootstocks imparted moderate to high resistance against bacterial wilt in both the scions, while tomato (cultivated or wild) rootstocks did not improve disease resistance, except ‘Anagha’, which provided resistance to scion cv. ‘Cheramy’. In general, scion cv. ‘Cheramy’, grafted or non-grafted, showed superior growth, yield and fruit quality compared to ‘Sheeja’. The most productive graft combinations for both the cultivars involved resistant rootstocks, i.e., ‘Sheeja’ onto eggplant rootstock ‘Surya’, and ‘Cheramy’ onto tomato rootstock ‘Anagha’. Fruit quality attributes such as ascorbic acid and lycopene contents were considerably higher, and the total soluble solids (TSS) content was considerably lower in scion cv. ‘Cheramy’, whether grafted or non-grafted, than those involving scion cv. ‘Sheeja’. The grafting effect on fruit chemical quality attributes was not promising, except grafting ‘Sheeja’ onto ‘Sopim’ for TSS, ‘Sheeja’ onto ‘Sotor’ for lycopene and ‘Cheramy’ onto ‘Ponny’ for total phenols, though no clear connection with disease incidence was in these grafts. Conclusively, eggplant rootstock imparted wilt resistance, while both eggplant and tomato rootstock grafting was beneficial to both scion cultivars in boosting the overall production and economic gains, especially for ‘Cheramy’ grafted onto ‘Anagha’ rootstock under bacterial wilt infested soil of greenhouse.