scholarly journals Marivirga lumbricoides sp. nov., a marine bacterium isolated from the South China Sea

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 452-456 ◽  
Author(s):  
Yongle Xu ◽  
Rui Zhang ◽  
Qipei Li ◽  
Keshao Liu ◽  
Nianzhi Jiao
Keyword(s):  
South China Sea ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
South China ◽  
Type Species ◽  
The South China Sea ◽  
Rrna Gene ◽  
Content Type ◽  
China Sea ◽  
Link Type

A novel, aerobic, heterotrophic, orange-pigmented, Gram-staining-negative, rod-shaped, gliding bacterial strain, designated JLT2000T, was isolated from surface water of the South China Sea. The strain was oxidase- and catalase-positive. The major cellular fatty acids of strain JLT2000 T were C12 : 0, iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 0. MK-7 was the major respiratory quinone and the major polar lipids were phosphatidylcholine and phosphatidylethanolamine. The genomic DNA G+C content of strain JLT2000T was 37.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JLT2000T formed a branch within the genus Marivirga , but was clearly separated from the two established species of this genus, Marivirga tractuosa and Marivirga sericea . The 16S rRNA gene sequence similarity of strain JLT2000T with the type strains of these two species was 95.8 % and 96.1 %, respectively. Strain JLT2000T had a shorter cell length and wider growth range in different temperatures and salinities than those of Marivirga tractuosa NBRC 15989T and Marivirga sericea NBRC 15983T. In addition, strain JLT2000T could utilize more carbon sources and hydrolyse more polymers than Marivirga tractuosa NBRC 15989T and Marivirga sericea NBRC 15983T. Based on this polyphasic analysis, strain JLT2000T represents a novel species of the genus Marivirga , for which the name Marivirga lumbricoides sp. nov. is proposed. The type strain is JLT2000T ( = JCM 18012T = CGMCC 1.10832T).

Sinobacterium caligoides gen. nov., sp. nov., a new member of the family Oceanospirillaceae isolated from the South China Sea, and emended description of Amphritea japonica

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2095-2100 ◽  
Author(s):  
Jianqiang Su ◽  
Yanyan Zhou ◽  
Qiliang Lai ◽  
Xinyi Li ◽  
Peiyan Dong ◽  
...  
Keyword(s):  
South China Sea ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
South China ◽  
Type Species ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Emended Description

A taxonomic study was carried out on strain SCSWE24T, isolated from a seawater sample collected from the South China Sea. Cells of strain SCSWE24T were Gram-negative, rod-shaped, non-motile, moderately halophilic and capable of reducing nitrate to nitrite. Growth was observed at salinities from 1.5 to 4.5 % and at 4–37 °C; it was unable to degrade gelatin. The dominant fatty acids (>15 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 50.4 %) and C16 : 0 (21.1 %). The G+C content of the chromosomal DNA was 58.8 mol%. 16S rRNA gene sequence comparisons showed that strain SCSWE24T was most closely related to an uncultured bacterium clone Tun3b.F5 (98 %; GenBank accession no. FJ169216), and showed 92 % similarity to an endosymbiont bacterium from the bone-eating worm Osedax mucofloris (clone Omu 9 c4791; FN773233). Levels of similarity between strain SCSWE24T and type strains of recognized species in the family Oceanospirillaceae were less than 93 %; the highest similarity was 92 %, to both Amphritea japonica JAMM 1866T and ‘Oceanicoccus sagamiensis’ PZ-5. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SCSWE24T formed a distinct evolutionary lineage within the family Oceanospirillaceae . Strain SCSWE24T was distinguishable from members of phylogenetically related genera by differences in several phenotypic properties. On the basis of the phenotypic and phylogenetic data, strain SCSWE24T represents a novel species of a new genus, for which the name Sinobacterium caligoides gen. nov., sp. nov. is proposed. The type strain of Sinobacterium caligoides is SCSWE24T ( = CCTCC AB 209289T  = LMG 25705T  = MCCC 1F01088T). An emended description of Amphritea japonica is also provided.

scholarly journals Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1712-1717 ◽  
Author(s):  
Daochen Zhu ◽  
Shoko-Hosoi Tanabe ◽  
Changxiao Xie ◽  
Daiske Honda ◽  
Jianzhong Sun ◽  
...  
Keyword(s):  
South China Sea ◽  
16S Rrna ◽  
South China ◽  
Type Species ◽  
The South China Sea ◽  
Diaminopimelic Acid ◽  
The South ◽  
Content Type ◽  
China Sea ◽  
Link Type

An alkaliphilic and halotolerant Gram-stain-positive bacterium, which was isolated from sediment samples from the South China Sea, was subjected to a taxonomic study. The isolate, strain L1T, grew well at a wide range of temperatures and pH values, 10.0–45.0 °C and pH 6–11, with optima at 30 °C and pH 9.0, respectively. The growth of strain L1T occurred at total salt concentrations of 0–10 % (w/v) with an optimum at 2 % (w/v). Phylogenetic analysis based on 16S rRNA sequence comparison indicated that the isolate represented a member of the genus Bacillus . The strains most closely related to strain L1T were Bacillus nanhaiisediminis JCM 16507T, Bacillus halodurans DSM 497T and Bacillus pseudofirmus DSM 8715T, with 16S rRNA similarities of 96.5 %, 95.9 % and 95.7 %, respectively. DNA–DNA hybridization of strain L1T with the type strains of the most closely related species, B. nanhaiisediminis JCM 16507T, B. halodurans DSM 497T and B. pseudofirmus DSM 8715T, showed reassociation values of about 21.7 %, 14.3 % and 13.9 %, respectively. The DNA G+C content of strain L1 T was 40.76 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids of strain L1T were iso-C14 : 0 and anteiso-C15 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the phenotypic and phylogenetic characteristics, it is proposed that strain L1T ( = JCM 18543T = DSM 26145T) should be classified as the type strain of Bacillus ligniniphilus sp. nov.

scholarly journals Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter

2007 ◽  
Vol 57 (2) ◽  
pp. 219-222 ◽  
Author(s):  
Jiao-Yan Ying ◽  
Zhi-Pei Liu ◽  
Bao-Jun Wang ◽  
Xin Dai ◽  
Su-Sheng Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
South China Sea ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
Emended Description

A novel marine bacterial strain, HY1T, was isolated from sediment of the South China Sea. The strain was aerobic and heterotrophic and formed saffron yellow-pigmented colonies on marine agar 2216. Cells were non-motile, Gram-negative rods, frequently occurring in chains. blastn searches revealed that the 16S rRNA gene sequence of strain HY1T showed high similarity with those of members of the genera Gillisia (91.7–93.8 %) and Salegentibacter (92.6–93.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain clustered with members of both Salegentibacter and Gillisia and phylogenetic trees constructed using three different methods (neighbour-joining, maximum-parsimony and minimum-evolution) indicated that strain HY1T clustered more frequently with members of the genus Salegentibacter. The DNA G+C content of strain HY1T was 44.4 mol% and its major cellular fatty acids (⩾5 % of the total fatty acids) were iso-15 : 1 (5.0 %), iso-15 : 0 (6.8 %), anteiso-15 : 0 (6.4 %), 15 : 0 (10.4 %), iso-16 : 0 (13.5 %), summed feature 3 (comprising iso-15 : 0 2-OH and/or 16 : 1ω7c; 6.3 %), iso-17 : 0 3-OH (5.2 %) and 17 : 0 2-OH (5.0 %). Cells contained menaquinone 6. Based on the phylogenetic and phenotypic analyses, strain HY1T should be classified as representing a novel species within the genus Salegentibacter, for which the name Salegentibacter catena sp. nov. is proposed. The type strain is HY1T (=CGMCC 1.6101T=JCM 14015T). Based on this study and on previously described Salegentibacter species, an emended description of the genus Salegentibacter is given.

scholarly journals Actinomadura craniellae sp. nov., isolated from a marine sponge in the South China Sea

2019 ◽  
Vol 69 (4) ◽  
pp. 1207-1212 ◽  
Author(s):  
Lei Li ◽  
Qi-hang Xu ◽  
Xiao-tian Wang ◽  
Hou-wen Lin ◽  
Yan-hua Lu
Keyword(s):  
South China Sea ◽  
South China ◽  
Marine Sponge ◽  
Type Species ◽  
The South China Sea ◽  
Diaminopimelic Acid ◽  
The South ◽  
Content Type ◽  
China Sea ◽  
Link Type

A novel marine actinomycete, designated LHW63021T, was isolated from a marine sponge, genus Craniella, collected in the South China Sea. A polyphasic approach was applied to characterize the taxonomic position of this strain. The strain was found to have scarce aerial mycelia that differentiated into spore chains. The cell-wall hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid. Glucose, galactose, mannose and madurose were found in the whole-cell hydrolysates. The dominant polar lipids were phosphatidylinositol and diphosphatidylglycerol. MK-9(H6) and MK-9(H8) were the predominant menaquinones. The major fatty acids were iso-C16 : 0, iso-C18 : 0, 10-methyl C17 : 0 and C18 : 1 ω9c. The DNA G+C content based on the draft genome sequence was 72.0 mol%. 16S rRNA gene sequence analysis indicated that strain LHW63021T was a member of the genus Actinomadura and had the highest similarity to Actinomadura echinospora DSM 43163T (97.3 %). Phylogenetic trees supported their close relationship. The average nucleotide identity and digital DNA–DNA hybridization values between the whole genome sequences of strain LHW63021T and A. echinospora DSM 43163T were 79.13 and 23.20 %, respectively. The evidence from the polyphasic study shows that strain LHW63021T represents a novel species of the genus Actinomadura , for which the name Actinomadura craniellae sp. nov. is proposed. The type strain is LHW63021T (=DSM 106125T=CCTCC AA 2018015T).

Shewanella nanhaiensis sp. nov., a marine bacterium isolated from sediment of South China Sea, and emended descriptions of Shewanella woodyi, Shewanella hanedai and Shewanella canadensis

2021 ◽  
Vol 71 (12) ◽  
Author(s):  
Wen-Rui Cao ◽  
Xue Li ◽  
Yuan-Yuan Sun ◽  
Ming-Yu Jiang ◽  
Xiao-Dong Xu ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Type Species ◽  
Northern South China Sea ◽  
Phylogenomic Analysis ◽  
Rrna Gene ◽  
Content Type ◽  
China Sea ◽  
Link Type ◽  
Shewanella Woodyi

A Gram-stain-negative, motile, facultative anaerobic and rod-shaped bacterium, designated strain NR704-98T, was isolated from marine sediment of the northern South China Sea. Cells were positive for oxidase and catalase activity. Growth was observed at 4–30 °C (optimum 20–25 °C), at pH 6–9 (pH 7) and with 0.5–7 % NaCl (2 %). The 16S rRNA gene-based phylogenetic analysis revealed that the nearest phylogenetic neighbours of strain NR704-98T were Shewanella woodyi MS32T (97.9 %), Shewanella hanedai 281T (97.1 %), Shewanella sediminis HAW-EB3T (96.8 %) and Shewanella canadensis HAW-EB2T (96.7 %). Based on the results of phylogenomic analysis, the average nucleotide identity and the digital DNA–DNA hybridization values between strain NR704-98T and the previously mentioned type strains of species of the genus Shewanella were in the range of 74.9–93.1 % and 20.6–51.4 %, respectively. The respiratory quinones were Q-7 and Q-8. The predominant fatty acids (>10 %) of strain NR704-98T were C16 : 0, summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c) and iso-C15 : 0. Phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminophospholipids and five unidentified lipids were detected in strain NR704-98T. Based on the phylogenetic and phenotypic characteristics, strain NR704-98T is considered to represent a novel species of the genus Shewanella , for which the name Shewanella nanhaiensis sp. nov. is proposed. The type strain is NR704-98T (=KCTC 82799T=MCCC 1K06091T).

scholarly journals Zhouia amylolytica gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from sediment of the South China Sea

2006 ◽  
Vol 56 (12) ◽  
pp. 2825-2829 ◽  
Author(s):  
Zhi-Pei Liu ◽  
Bao-Jun Wang ◽  
Xin Dai ◽  
Xing-Yu Liu ◽  
Shuang-Jiang Liu
Keyword(s):  
South China Sea ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
China Sea ◽  
The Family

Three Gram-negative, non-spore-forming strains were isolated from sediment from the South China Sea, China, and their taxonomic positions were investigated using a polyphasic approach. Strains HN-171T, HN-172 and HN-181 grew optimally at 30 °C, in the presence of 4.5–5.0 NaCl % (w/v) and at pH 7.2–7.4. They contained MK-6 as the predominant respiratory quinone and contained iso-C15 : 1 G, iso-C15 : 0, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c/t) and C15 : 0 as the major fatty acids. The DNA G+C content of strain HN-171T was 34.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain HN-171T, together with strains HN-172 and HN-181, formed a distinct evolutionary lineage within the family Flavobacteriaceae. The 16S rRNA gene sequences of strains HN-171T, HN-172 and HN-181 shared 99.8–100 % similarity with each other, and the sequence of strain HN-171T exhibited similarity values below 90.2 % with those of other members of the family Flavobacteriaceae. The closest relative of HN-171T was Coenonia anatina LMG 14382T (90.2 %). On the basis of their phenotypic and phylogenetic properties, the three isolates represent a novel genus and a novel species, for which the name Zhouia amylolytica gen. nov., sp. nov. is proposed. The type strain is HN-171T (=CGMCC 1.6114T=JCM 14016T).

scholarly journals Nitrincola iocasae sp. nov., a bacterium isolated from sediment collected at a cold seep field in the South China Sea

2020 ◽  
Vol 70 (9) ◽  
pp. 4897-4902 ◽  
Author(s):  
Huan Zhang ◽  
Hao Wang ◽  
Hao Chen ◽  
Qinglei Sun ◽  
Zhaoshan Zhong ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Type Species ◽  
The South China Sea ◽  
Cold Seep ◽  
The South ◽  
Content Type ◽  
China Sea ◽  
Link Type ◽  
A Genome

A novel bacterium, designated strain KXZD1103T, was isolated from sediment collected at a cold seep field of the Formosa Ridge in the South China Sea. Cells were Gram-stain-negative, facultatively anaerobic, motile, oxidase- and catalase-positive, and grew optimally at 28 °C, pH 6.0–pH 7.0 and in the presence of 1–3 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (C18 : 1  ω7c/C18 : 1  ω6c), summed feature 3 (C16 : 1  ω7c/C16 : 1  ω6c) and C16 : 0. The major respiratory ubiquinone was Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Analysis of 16S rRNA gene sequences revealed that strain KXZD1103T grouped with members of the genus Nitrincola , with Nitrincola lacisaponensis 4CAT (98.1 % sequence similarity) and Nitrincola schmidtii R4-8T (97.7 %) as its closest neighbours. Genome sequencing revealed a genome size of 4.17 Mb and a DNA G+C content of 50.1 %. Genomic average nucleotide identity values for strain KXZD1103T with the type strains within the genus Nitrincola ranged from 71.0 to 75.7 %, while the in silico DNA–DNA hybridization values for strain KXZD1103T with these strains ranged from 16.1 to 21.6 %. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, strain KXZD1103T is considered to represent a novel species of the genus Nitrincola , for which the name Nitrincola iocasae sp. nov. is proposed. The type strain is KXZD1103T (=KCTC 72678T=MCCC 1K04283T).

scholarly journals Prauserella marina sp. nov., isolated from ocean sediment of the South China Sea

2010 ◽  
Vol 60 (4) ◽  
pp. 985-989 ◽  
Author(s):  
Jian Wang ◽  
Yan Li ◽  
Jiang Bian ◽  
Shu-Kun Tang ◽  
Biao Ren ◽  
...  
Keyword(s):  
South China Sea ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
Ocean Sediment ◽  
Actinomycete Strain

A novel actinomycete strain, designated MS498T, was isolated from an ocean sediment sample collected from the South China Sea. It was subjected to a polyphasic analysis to determine its taxonomic position. The phylogenetic tree based on 16S rRNA gene sequences showed that strain MS498T had the highest similarity (96.5 %) with members of the genus Prauserella and was loosely associated with Prauserella rugosa DSM 43194T and Saccharomonospora halophila DSM 44411T. Based on 16S rRNA gene sequence analysis, phenotypic characteristics and chemotaxonomic data, the new isolate is proposed to represent a novel species of the genus Prauserella, named Prauserella marina sp. nov. (type strain MS498T=CCTCC AA 208056T =DSM 45268T).

Muricauda onchidii sp. nov., isolated from a marine invertebrate from South China Sea, and transfers of Flagellimonas algicola, Flagellimonas pacifica and Flagellimonas maritima to Muricauda algicola comb. nov., Muricauda parva nom. nov. and Muricauda aurantiaca nom. nov., respectively, and emended description of the genus Muricauda

2021 ◽  
Vol 71 (9) ◽  
Author(s):  
Jinyou Liang ◽  
Qi Yin ◽  
Xiaoli Zheng ◽  
Yu Wang ◽  
Zhi-Man Song ◽  
...  
Keyword(s):  
South China Sea ◽  
16S Rrna ◽  
South China ◽  
Type Species ◽  
Marine Invertebrate ◽  
Polar Lipids ◽  
Content Type ◽  
China Sea ◽  
Link Type ◽  
Emended Description

An aerobic, Gram-stain-negative, rod-shaped and non-motile strain (XY-359T) was isolated from the mouth of a marine invertebrate Onchidium species from the South China Sea. It grew at pH 6.0–8.5 (optimum, pH 7.5), at 15−37 °C (optimum, 30 °C) and in the presence of 0.5–4.5 % (w/v) NaCl (optimum, 2.5 %). It could not hydrolyse Tweens 20, 40, 60 or 80 and no flexirubin-type pigments were produced. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid, six unidentified phospholipids and two unidentified polar lipids. The major fatty acids were iso-C17:0 3-OH, iso-C15:1 G and iso-C15:0 3-OH. The respiratory quinone was MK-6. Strain XY-359T showed the greatest degree of 16S rRNA sequence similarity to Flagellimonas algicola AsT0115T (96.54 %), followed by Muricauda flava DSM 22638T (96.27 %). Phylogenetic analysis based on 16S rRNA gene sequences and 31 core genes indicated that strain XY-359T belongs to the genus Muricauda . The genome size of strain XY-359T was 4 207 872 bp, with 39.1 mol% of DNA G+C content. The average nucleotide identity and digital DNA–DNA hybridization values between strain XY-359T and F. algicola AsT0115T were 74.58 % and 18.5 %, respectively, and those between strain XY-359T and M. flava DSM 22638T were 74.2 % and 18.3 %. The combined phenotypic, chemotaxonomic and phylogenetic data suggest that strain XY-359T represents a novel species of the genus Muricauda , for which the name Muricauda onchidii sp. nov. is proposed. The type strain is XY-359T (=MCCC 1K03658T =KCTC 72218T). Moreover, based on the proposal of nesting Spongiibacterium and Flagellimonas within Muricauda by García (Validation List No. 193) and the analyses of phylogenetic trees and average amino acid identities in this study, the transfers of F. algicola , F. pacifica and F. maritima to the genus Muricauda as Muricauda algicola comb. nov., Muricauda parva nom. nov. and M. aurantiaca nom. nov., respectively, are proposed, with an emended description of the genus Muricauda .

scholarly journals Devosia marina sp. nov., isolated from deep seawater of the South China Sea, and reclassification of Devosia subaequoris as a later heterotypic synonym of Devosia soli

2020 ◽  
Vol 70 (5) ◽  
pp. 3062-3068 ◽  
Author(s):  
Yang Liu ◽  
Juan Du ◽  
Jun Zhang ◽  
Qiliang Lai ◽  
Zongze Shao ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
South China Sea ◽  
South China ◽  
Type Species ◽  
The South China Sea ◽  
The South ◽  
Deep Seawater ◽  
Content Type ◽  
China Sea ◽  
Link Type

A Gram-stain-negative, aerobic, rod-shaped and motile bacterial strain, designated L53-10-65T, was isolated from deep seawater of the South China Sea. Strain L53-10-65T was found to grow at 4–41 °C (optimum, 28 °C), at pH 5.0–9.0 (pH 7.0–8.0) and in 0–7 % (w/v) NaCl (2 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain L53-10-65T fell within the genus Devosia , with the highest similarity to Devosia indica IO390501T (98.4 %), followed by ‘ Devosia lucknowensis ’ L15T (97.7 %) and Devosia riboflavina IFO 13584T (96.8 %). The digital DNA–DNA hybridization (dDDH) values between strain L53-10-65T and the three relatives above were 43.7, 21.5 and 20.9 %, respectively; the average nucleotide identity (ANI) values were 91.3, 78.4 and 76.8 %, respectively. These values were below the 70 % dDDH and 95–96 % ANI thresholds for bacterial species delineation. The major cellular fatty acids of strain L53-10-65T were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), C18 : 1 ω7c 11-methyl and C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The respiratory quinone was identified as Q-10. The DNA G+C content of strain L53-10-65T was 61.3 mol%. Based on the results of phylogenetic analysis and distinctive phenotypic characteristics, strain L53-10-65T is concluded to represent a novel species of the genus Devosia , for which the name Devosia marina sp. nov. is proposed. The type strain of the species is L53-10-65T (=MCCC 1A05139T=KCTC 72888T). Moreover, we propose that Devosia subaequoris is a later heterotypic synonym of Devosia soli based on the present results.

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