scholarly journals Schlegelella aquatica sp. nov., a novel thermophilic bacterium isolated from a hot spring

2006 ◽  
Vol 56 (12) ◽  
pp. 2793-2797 ◽  
Author(s):  
Yi-Ju Chou ◽  
Shih-Yi Sheu ◽  
Der-Shyan Sheu ◽  
Jih-Terng Wang ◽  
Wen-Ming Chen
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Moderately Thermophilic ◽  
Physiological And Biochemical Characteristics ◽  
Southern Taiwan ◽  
Monophyletic Branch

A moderately thermophilic bacterial strain designated wcf1T, isolated from a hot spring located in the Tainan area, southern Taiwan, was characterized using a polyphasic approach. The cells were Gram-negative, non-pigmented, rod-shaped, non-spore-forming and motile. Phylogenetic analysis using 16S rRNA gene sequences showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Schlegelella; its only close neighbour was the type strain of Schlegelella thermodepolymerans, K14T (97.8 %). The isolate was clearly distinguishable from other strains using phylogenetic analysis, DNA–DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics. It was evident from the genotypic and phenotypic data that strain wcf1T represents a novel species in the genus Schlegelella, for which the name Schlegelella aquatica sp. nov. is proposed, with the type strain wcf1T (=BCRC 17557T=LMG 23380T).

scholarly journals Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan

2007 ◽  
Vol 57 (7) ◽  
pp. 1625-1629 ◽  
Author(s):  
Kuan-Yin Lin ◽  
Shih-Yi Sheu ◽  
Poh-Shing Chang ◽  
Jang-Cheon Cho ◽  
Wen-Ming Chen
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Evolutionary Radiation ◽  
Southern Taiwan ◽  
Monophyletic Branch

A short-rod-shaped, Gram-negative, non-motile bacterial strain, designated AZO-CT, was isolated from a sample of seawater collected from the Eluanbi coast of Pingtung County in southern Taiwan and was characterized by using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Oceanicola in the order Rhodobacterales of the Alphaproteobacteria. The closest neighbours were Oceanicola batsensis HTCC2597T (95.6 % similarity), Oceanicola nanhaiensis SS011B1-20T (94.5 %) and Oceanicola granulosus HTCC2516T (94.0 %). The predominant fatty acid was 18 : 1ω7c (49.1 %), and significant amounts of 19 : 0 cyclo (24.6 %) and 16 : 0 (14.7 %) were present. The DNA–DNA relatedness of the strain with respect to recognized species of the genus Oceanicola was less than 70 %. The isolate was also distinguishable from members of the genus Oceanicola on the basis of phenotypic and biochemical characteristics. It is evident from the genotypic, chemotaxonomic and phenotypic data, therefore, that strain AZO-CT represents a novel species of the genus Oceanicola, for which the name Oceanicola marinus sp. nov. is proposed. The type strain is AZO-CT (=LMG 23705T=BCRC 17591T).

Albidovulum xiamenense sp. nov., a moderately thermophilic bacterium from a terrestrial hot spring

2012 ◽  
Vol 62 (Pt_7) ◽  
pp. 1609-1612 ◽  
Author(s):  
Decui Yin ◽  
Jing Xiao ◽  
Jingqun Ao ◽  
Chunxiang Ai ◽  
Xinhua Chen
Keyword(s):  
Phylogenetic Analysis ◽  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Republic Of China ◽  
Link Type ◽  
Terrestrial Hot Spring

An aerobic, motile, moderately thermophilic, rod-shaped bacterium, strain YBY-7T, was isolated from a terrestrial hot spring of a garden exhibition located in Xiamen City, Fujian Province, People’s Republic of China. Cells of strain YBY-7T were Gram-negative, irregular rods, 2–6 µm long and 0.4–0.6 µm wide, with polar flagella, and the organism formed beige colonies. The temperature and pH ranges for growth of strain YBY-7T were 28–65 °C (optimum 50–58 °C) and pH 6.5–9.5 (optimum pH 7.5–8.5). Growth occurred in the presence of 5.5 % NaCl (optimum 3.0 %). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that Albidovulum inexpectatum FRR-10T was its closest neighbour (95.9 % similarity). Ubiquinone (Q-10) was the sole respiratory quinone and the DNA G+C content of strain YBY-7T was 70.6 mol%. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were C18 : 1ω7c (29.3 %), C19 : 0 cyclo ω8c (25.6 %), C18 : 0 (23.6 %) and C16 : 0 (9.6 %). Based on its physiological characteristics and our phylogenetic analysis, we propose that strain YBY-7T represents a novel species of the genus Albidovulum , for which the name Albidovulum xiamenense sp. nov. is proposed, with the type strain YBY-7T ( = MCCC 1A06317T  = CGMCC 1.10789T  = LMG 26247T).

Luteimonas huabeiensis sp. nov., isolated from stratum water

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3352-3357 ◽  
Author(s):  
Gang Wu ◽  
Yang Liu ◽  
Qing Li ◽  
Huijing Du ◽  
Jing You ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Type Species ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Physiological And Biochemical Characteristics ◽  
Taxonomic Approach ◽  
Type Strains ◽  
Physiological And Biochemical

A yellow-coloured bacterial strain, designated HB2T, isolated from stratum water was investigated using a polyphasic taxonomic approach. Cells were Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was a member of the genus Luteimonas , its three closest neighbours being Luteimonas aquatica BCRC 17731T (97.5 % similarity), Luteimonas marina JCM 12488T (97.3 %) and Luteimonas aestuarii DSM 19680T (96.9 %). Strain HB2T could clearly be distinguished from these type strains based on phylogenetic analysis, DNA–DNA hybridization, fatty acid composition and a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strain HB2T represents a novel species of the genus Luteimonas , for which the name Luteimonas huabeiensis sp. nov. is proposed. The type strain is HB2T ( = DSM 26429T = CICC 11005sT).

scholarly journals Sphingomonas morindae sp. nov., isolated from Noni (Morinda citrifolia L.) branch

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2817-2823 ◽  
Author(s):  
Yang Liu ◽  
Su Yao ◽  
Yong-Jae Lee ◽  
Yanhua Cao ◽  
Lei Zhai ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Novel Species ◽  
Morinda Citrifolia ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Phenotypic Data ◽  
Respiratory Quinone ◽  
Physiological And Biochemical Characteristics ◽  
Sphingomonas Wittichii ◽  
Taxonomic Approach

Two yellow bacterial strains, designated NBD5T and NBD8, isolated from Noni (Morinda citrifolia L.) branch were investigated using a polyphasic taxonomic approach. Cells were Gram-stain-negative, aerobic, non-spore-forming, non-motile and short rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences suggested that the strains were members of a novel species of the genus Sphingomonas, the seven closest neighbours being Sphingomonas oligoaromativorans SY-6T (96.9  % similarity), Sphingomonas polyaromaticivorans B2-7T (95.8  %), Sphingomonas yantingensis 1007T (94.9  %), Sphingomonas sanguinis IFO 13937T (94.7  %), Sphingomonas ginsenosidimutans Gsoil 1429T (94.6  %), Sphingomonas wittichii RW1T (94.6  %) and Sphingomonas formosensis CC-Nfb-2T (94.5  %). Strains NBD5T and NBD8 had sphingoglycolipid, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine as the major polar lipids, ubiquinone 10 as the predominant respiratory quinone, and sym-homospermidine as the major polyamine. Strains NBD5T and NBD8 were clearly distinguished from reference type strains based on phylogenetic analysis, DNA–DNA hybridization, fatty acid composition data analysis, and comparison of a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strains NBD5T and NBD8 represent a novel species of the genus Sphingomonas, for which the name Sphingomonas morindae sp. nov. is proposed. The type strain is NBD5T ( = DSM 29151T = KCTC 42183T = CICC 10879T).

scholarly journals Schleiferia thermophila gen. nov., sp. nov., a slightly thermophilic bacterium of the phylum ‘Bacteroidetes’ and the proposal of Schleiferiaceae fam. nov.

2011 ◽  
Vol 61 (10) ◽  
pp. 2450-2455 ◽  
Author(s):  
Luciana Albuquerque ◽  
Fred A. Rainey ◽  
M. Fernanda Nobre ◽  
Milton S. da Costa
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Respiratory Quinone ◽  
Optimum Growth Temperature ◽  
Physiological And Biochemical Characteristics ◽  
Gene Sequence Analysis ◽  
Major Respiratory Quinone

A bacterial isolate, with an optimum growth temperature of about 50 °C and an optimum pH for growth between 7.5 and 8.5, was recovered from a hot spring in the Furnas area of the Island of São Miguel in the Azores. The novel isolate is orange-pigmented, forms non-motile, rod-shaped cells that stain Gram-negative, is strictly aerobic, oxidase-positive and catalase-negative. The major fatty acids of strain TU-20T are 16 : 1ω6c, 17 : 0 iso 3-OH and 15 : 0 iso. Menaquinone 6 is the major respiratory quinone. Based on 16S rRNA gene sequence analysis, physiological and biochemical characteristics, we describe a novel species of a novel genus represented by strain TU-20T ( = DSM 21410T  = LMG 24594T) for which we propose the name Schleiferia thermophila gen nov., sp. nov. We also propose the family Schleiferiaceae fam. nov. to accommodate this new genus.

scholarly journals Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 849-855 ◽  
Author(s):  
Kadriye Inan ◽  
Sabriye Canakci ◽  
Ali Osman Belduz ◽  
Fikrettin Sahin
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Lipid Analysis ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Gene Sequence Analysis

Two Gram-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria, designated PDF25T and PDF30, were isolated from Karakoc hot spring in the province of Izmir, Turkey, and were characterized in order to determine their phylogenetic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genus Brevibacillus ; strain PDF25T showed highest sequence similarity to strain PDF30 (99.4 %) and Brevibacillus thermoruber DSM 7064T (98.5 %). The major fatty acids of strain PDF25T were iso-C15 : 0 (39.30 %), anteiso-C15 : 0 (26.10 %) and iso-C16 : 0 (14.75 %). Polar lipid analysis revealed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, and a variety of unidentified aminophospholipids, phospholipids and aminolipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 56.09 mol%. DNA–DNA hybridization experiments revealed 58 % relatedness between strain PDF25T and B. thermoruber DSM 7064T. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus , for which the name Brevibacillus aydinogluensis sp. nov. is proposed. The type strain is PDF25T ( = DSM 24395T = LMG 26289T).

Anoxybacillus vitaminiphilus sp. nov., a strictly aerobic and moderately thermophilic bacterium isolated from a hot spring

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4064-4071 ◽  
Author(s):  
Xin-Qi Zhang ◽  
Zhen-Li Zhang ◽  
Nan Wu ◽  
Xu-Fen Zhu ◽  
Min Wu
Keyword(s):  
Hot Spring ◽  
Novel Species ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Bacterium Strain

A strictly aerobic, Gram-stain-positive, motile and spore-forming bacterium, strain 3nP4T, was isolated from the Puge hot spring located in the south-western geothermal area of China. Strain 3nP4T grew at 38–66 °C (optimum 57–60 °C), at pH 6.0–9.3 (optimum 7.0–7.5) and with 0–4 % (w/v) NaCl (optimum 0–0.5 %). Phylogenetic analysis of 16S rRNA gene sequences, as well as DNA–DNA relatedness values, indicated that the isolate represents a novel species of the genus Anoxybacillus , related most closely to Anoxybacillus voinovskiensis DSM 12111T. Strain 3nP4T had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid as major polar lipids and iso-C15 : 0 and iso-C17 : 0 as major fatty acids, which are both typical chemotaxonomic characteristics of the genus Anoxybacillus . The mean DNA G+C content of strain 3nP4T was 39.2±0.95 mol% (HPLC). A distinctive characteristic of the novel isolate was its extreme reliance on vitamin mixture or yeast extract for growth. Based on data from this taxonomic study using a polyphasic approach, strain 3nP4T is considered to represent a novel species of the genus Anoxybacillus , for which the name Anoxybacillus vitaminiphilus sp. nov. is proposed. The type strain is 3nP4T ( = CGMCC 1.8979T = JCM 16594T).

scholarly journals Comamonas odontotermitis sp. nov., isolated from the gut of the termite Odontotermes formosanus

2007 ◽  
Vol 57 (4) ◽  
pp. 887-891 ◽  
Author(s):  
Jui-Hsing Chou ◽  
Shih-Yi Sheu ◽  
Kuan-Yin Lin ◽  
Wen-Ming Chen ◽  
A. B. Arun ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Cell Protein ◽  
Rrna Gene ◽  
Comamonas Testosteroni ◽  
Phenotypic Data ◽  
Physiological And Biochemical Characteristics ◽  
Taxonomic Approach ◽  
Odontotermes Formosanus ◽  
Monophyletic Branch

A bacterial strain, designated Dant 3-8T, isolated from the gut of the termite Odontotermes formosanus, was investigated by a polyphasic taxonomic approach. The cells were rod-shaped, Gram-negative, non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analyses using the 16S rRNA gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Comamonas, its closest neighbours being Comamonas testosteroni DSM 50244T (96.4 % sequence similarity), Comamonas koreensis KCTC 12005T (96.0 %) and Comamonas terrigena DSM 7099T (96.2 %). Strain Dant 3-8T was clearly distinguished from all of these strains by using phylogenetic analysis, DNA–DNA hybridization, whole-cell protein profiles, fatty acid composition data and a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that Dant 3-8T represents a novel species in the genus Comamonas, for which the name Comamonas odontotermitis sp. nov. is proposed. The type strain is Dant 3-8T (=BCRC 17576T=LMG 23579T).

scholarly journals Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste

2007 ◽  
Vol 57 (4) ◽  
pp. 741-744 ◽  
Author(s):  
Chiu-Chung Young ◽  
Peter Kämpfer ◽  
Wen-Ming Chen ◽  
Wen-Shao Yen ◽  
A. B. Arun ◽  
...  
Keyword(s):  
Food Waste ◽  
Type Strain ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Physiological Data ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Moderately Thermophilic ◽  
Gene Sequence Analysis ◽  
Bacterium Strain

A yellow-pigmented, Gram-negative, rod-shaped bacterium (strain CC-YY255T) was isolated from compost generated from food waste collected from Kinmen County, Taiwan. 16S rRNA gene sequence analysis indicated that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Luteimonas; its closest neighbour was the type strain of Luteimonas mephitis (94.4 % sequence similarity). The isolate was distinguished from Luteimonas mephitis on the basis of several phenotypic properties. The organism utilized glucose, maltose, gentiobiose, melibiose and turanose and only a few organic acids (acetate, propionate) and amino acids (l-alanyl glycine, glycyl l-aspartic acid and glycyl l-glutamic acid) as substrates. The fatty acid profile was slightly different from that reported for Luteimonas mephitis. It is evident from the genotypic, chemotaxonomic and physiological data presented that strain CC-YY255T represents a novel species of the genus Luteimonas, for which the name Luteimonas composti sp. nov. is proposed. The type strain is CC-YY255T (=CCUG 53595T=CIP 109311T=BCRC 17598T).

scholarly journals Transfer of the misnamed [Alysiella] sp. IAM 14971 (=ATCC 29468) to the genus Moraxella as Moraxella oblonga sp. nov.

2005 ◽  
Vol 55 (1) ◽  
pp. 331-334 ◽  
Author(s):  
Cheng-Hui Xie ◽  
Akira Yokota
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data

Phylogenetic analysis based on 16S rRNA gene sequences revealed that [Alysiella] sp. IAM 14971 (=ATCC 29468) is closely related to the genus Moraxella of the γ-Proteobacteria (96–97 % similarity). The newly obtained phenotypic data also indicate that [Alysiella] sp. IAM 14971 is distinct from the genus Alysiella and similar to the genus Moraxella. On the basis of these results, the strain should be classified in the genus Moraxella, as Moraxella oblonga sp. nov. The type strain is IAM 14971T (=ATCC 29468T).

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