scholarly journals Schleiferia thermophila gen. nov., sp. nov., a slightly thermophilic bacterium of the phylum ‘Bacteroidetes’ and the proposal of Schleiferiaceae fam. nov.

2011 ◽  
Vol 61 (10) ◽  
pp. 2450-2455 ◽  
Author(s):  
Luciana Albuquerque ◽  
Fred A. Rainey ◽  
M. Fernanda Nobre ◽  
Milton S. da Costa
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Respiratory Quinone ◽  
Optimum Growth Temperature ◽  
Physiological And Biochemical Characteristics ◽  
Gene Sequence Analysis ◽  
Major Respiratory Quinone

A bacterial isolate, with an optimum growth temperature of about 50 °C and an optimum pH for growth between 7.5 and 8.5, was recovered from a hot spring in the Furnas area of the Island of São Miguel in the Azores. The novel isolate is orange-pigmented, forms non-motile, rod-shaped cells that stain Gram-negative, is strictly aerobic, oxidase-positive and catalase-negative. The major fatty acids of strain TU-20T are 16 : 1ω6c, 17 : 0 iso 3-OH and 15 : 0 iso. Menaquinone 6 is the major respiratory quinone. Based on 16S rRNA gene sequence analysis, physiological and biochemical characteristics, we describe a novel species of a novel genus represented by strain TU-20T ( = DSM 21410T  = LMG 24594T) for which we propose the name Schleiferia thermophila gen nov., sp. nov. We also propose the family Schleiferiaceae fam. nov. to accommodate this new genus.

scholarly journals Tepidicella xavieri gen. nov., sp. nov., a betaproteobacterium isolated from a hot spring runoff

2006 ◽  
Vol 56 (4) ◽  
pp. 907-912 ◽  
Author(s):  
Luis França ◽  
Fred A. Rainey ◽  
M. Fernanda Nobre ◽  
Milton S. da Costa
Keyword(s):  
New Genus ◽  
Hot Spring ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Optimum Growth Temperature ◽  
Spring Runoff ◽  
Physiological And Biochemical Characteristics ◽  
Major Respiratory Quinone

Strains TU-16T and TU-18, two non-pigmented bacterial isolates with an optimum growth temperature of about 45 °C and an optimum pH of about 8·5–9·0, were recovered from the Furnas geothermal area on the Island of São Miguel in the Azores. Phylogenetic analysis of the 16S rRNA gene sequence of these strains indicated that they represent a novel species in a new genus of the phylum Betaproteobacteria. The major fatty acids of strains TU-16T and TU-18 were 16 : 0 and 18 : 1ω7c. Ubiquinone 8 was the major respiratory quinone and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The novel isolates were aerobic; thiosulfate was oxidized to sulfate in the presence of a metabolizable carbon source. The organism assimilated organic acids and amino acids, but did not assimilate carbohydrates or polyols. Based on phylogenetic analyses and physiological and biochemical characteristics, it is proposed that strain TU-16T (=LMG 23030T=CIP 108724T) represents the type strain of a novel species in a new genus, Tepidicella xavieri gen. nov., sp. nov.

scholarly journals Lysobacter novalis sp. nov., isolated from fallow farmland soil

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3131-3136 ◽  
Author(s):  
Hina Singh ◽  
Juan Du ◽  
Kyung-Hwa Won ◽  
Jung-Eun Yang ◽  
Shahina Akter ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Gliding Motility ◽  
Rrna Gene ◽  
The Novel ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
Farmland Soil ◽  
Major Respiratory Quinone

A novel bacterial strain, designated THG-PC7T, was isolated from fallow farmland soil in Yongin, South Korea. Cells of strain THG-PC7T were Gram-stain-negative, dark yellow, aerobic, rod-shaped and had gliding motility. Strain THG-PC7T grew optimally at 25–35 °C, at pH 7 and in the absence of NaCl. Comparative 16S rRNA gene sequence analysis identified strain THG-PC7T as belonging to the genus Lysobacter, exhibiting highest sequence similarity with Lysobacter ximonensis KCTC 22336T (98.7 %) followed by Lysobacter niastensis KACC 11588T (95.7 %). In DNA–DNA hybridization tests, DNA relatedness between strain THG-PC7T and its closest phylogenetic neighbour L. ximonensis was below 25 %. The DNA G+C content of the novel isolate was determined to be 62.5 mol%. Flexirubin-type pigments were found to be present. The major cellular fatty acids were determined to be iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C17 : 1ω9c. The major respiratory quinone was identified as ubiquonone-8 (Q8). The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospolipid. On the basis of results from DNA–DNA hybridization and the polyphasic data, strain THG-PC7T represents a novel species of the genus Lysobacter, for which the name Lysobacter novalis sp. nov. is proposed. The type strain is THG-PC7T( = KACC 18276T = CCTCC AB 2014319T).

scholarly journals Amphiplicatus metriothermophilus gen. nov., sp. nov., a thermotolerant alphaproteobacterium isolated from a hot spring

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2805-2811 ◽  
Author(s):  
Zhang Zhen-Li ◽  
Zhang Xin-Qi ◽  
Wu Nan ◽  
Zhang Wen-Wu ◽  
Zhu Xu-Fen ◽  
...  
Keyword(s):  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Respiratory Quinone ◽  
Content Type ◽  
North West ◽  
Link Type ◽  
Major Respiratory Quinone

A thermotolerant, Gram-strain-negative, non-spore-forming and strictly aerobic bacterium, designated GU51T, was isolated from Guhai hot spring in Jimsar county, Xinjiang province, north-west China. Each cell of strain GU51T consisted of an oval body and two symmetrical long (3–6 µm) prosthecae. The strain moved by polar flagellum. Oxidase and catalase were produced. Strain GU51T grew within the ranges of 37–65 °C (optimum 48–50 °C), 0.5–7.5 % (w/v) NaCl (optimum 2–3 %) and pH 6.0–9.0 (optimum pH 7.5). The major respiratory quinone detected was ubiquinone 10 (U-10) and the genomic DNA G+C content was 66.7±0.4 mol%. Major fatty acids (>5 %) were C16 : 0, C18 : 1ω7c and 11-methyl C18 : 1ω7c. The polar lipids consisted of diphosphatidylglycerol, five glycolipids, phosphatidylglycerol and an unknown phospholipid. Phylogenetic analysis showed the closest relatives of strain GU51T were members of the genus Parvularcula with 92.3 % 16S rRNA gene sequence similarity. On the basis of this polyphasic taxonomic characterization, it is suggested that strain GU51T represents a novel species of a new genus in the family ‘ Parvularculaceae ’, for which the name Amphiplicatus metriothermophilus gen. nov., sp. nov. is proposed. The type strain of the type species is GU51T ( = CGMCC 1.12710T = JCM 19779T).

scholarly journals Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome

2007 ◽  
Vol 57 (5) ◽  
pp. 1050-1054 ◽  
Author(s):  
Seung Seob Bae ◽  
Kae Kyoung Kwon ◽  
Sung Hyun Yang ◽  
Hee-Soon Lee ◽  
Sang-Jin Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Bacterium ◽  
Optimal Growth ◽  
Rrna Gene ◽  
The Novel ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Major Respiratory Quinone

A marine bacterium, DOKDO 007T, was isolated from the rhizosphere of the marine alga Ecklonia kurome collected from Dokdo Island, Korea, in October 2004. The strain produced orange-coloured colonies on marine agar 2216. 16S rRNA gene sequence analysis indicated that the novel isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarities with members of the genus Muricauda (92.0–94.0 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genera Muricauda and Costertonia. Cells were aerobic, Gram-negative rods producing non-diffusible carotenoid pigments. In contrast to all other members of the family Flavobacteriaceae, cells of DOKDO 007T were motile by means of a polar flagellum. Optimal growth occurred in the presence of 3.5–4 % (w/v) sea salts (corresponding to 2.7–3.1 % NaCl), at pH 8 and at temperatures of 26–29 °C. The novel strain required Ca2+ ions in addition to NaCl for growth. The dominant fatty acids were iso-15 : 0, iso-15 : 1ω10c and 10-methyl-16 : 0. The major respiratory quinone was MK-6. The DNA G+C content was 56.3 mol%, an unusually high value for members of the family Flavobacteriaceae. On the basis of these polyphasic taxonomic data, strain DOKDO 007T should be classified as representing a new genus and novel species in the family Flavobacteriaceae, for which the name Flagellimonas eckloniae gen. nov., sp. nov. is proposed. The type strain is DOKDO 007T (=KCCM 42307T=JCM 13831T).

Phycicoccus cremeus sp. nov., isolated from forest soil, and emended description of the genus Phycicoccus

2011 ◽  
Vol 61 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Jia-Yue Zhang ◽  
Xing-Yu Liu ◽  
Shuang-Jiang Liu
Keyword(s):  
Forest Soil ◽  
Diaminopimelic Acid ◽  
Changbai Mountains ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Sequence Similarities

A bacterial strain, designated V2M29T, was isolated from forest soil collected in the Changbai Mountains, Heilongjiang Province, China. Cells of strain V2M29T were Gram-positive, rod-shaped (0.5–0.8 μm in diameter and 1.5–2.0 μm in length), strictly aerobic and non-motile. Colonies produced on R2A agar plates were creamy, smooth, circular and 0.3–0.8 mm in diameter. Strain V2M29T grew at 14–35 °C (optimum, 29 °C), at pH 4.1–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–7.0 % (w/v) NaCl (optimum, 0–2.0 %). Strain V2M29T contained MK-8(H4) as the major respiratory quinone and iso-C15 : 0 (20.9 %), iso-C16 : 0 (18.5 %), C17 : 1 ω8c (16.7 %) and 10-methyl-C17 : 0 (9.2 %) as the major cellular fatty acids. The cell-wall peptidoglycan type was based on meso-2,6-diaminopimelic acid and the acyl type of the muramic acid was acetyl. Diphosphatidylglycerol, phosphatidylinositol and six unknown glycolipids were the major polar lipids. The G+C content of the DNA was 72.0 mol% (T m). 16S rRNA gene sequence analysis indicated that strain V2M29T was phylogenetically related to members of the genus Phycicoccus, with sequence similarities ranging from 96.6–97.8 %. The DNA–DNA relatedness values of strain V2M29T to Phycicoccus dokdonensis DS-8T and Phycicoccus bigeumensis DSM 19264T were 32.5±3.3 % and 29.2±2.3 %, respectively. Based on these results, it is concluded that strain V2M29T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus cremeus sp. nov. is proposed, with V2M29T (=CGMCC 1.6963T =NBRC 104261T) as the type strain.

scholarly journals Jeotgalibacillus soli sp. nov., a Gram-stain-positive bacterium isolated from soil

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 608-612 ◽  
Author(s):  
Sofia Cunha ◽  
Igor Tiago ◽  
Gabriel Paiva ◽  
Fernanda Nobre ◽  
Milton S. da Costa ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Rrna Gene ◽  
Optimum Growth ◽  
Respiratory Quinone ◽  
Optimum Growth Temperature ◽  
Gram Staining ◽  
Physiological And Biochemical Characteristics ◽  
Major Respiratory Quinone ◽  
Diamino Acid ◽  
Physiological And Biochemical

A Gram-staining-positive, motile, rod-shaped, spore-forming bacterium, designated P9T, was isolated from soil in Portugal. This organism was aerobic and catalase- and oxidase-positive. It had an optimum growth temperature of about 35 °C and an optimum growth pH of about 8.0–8.5, and grew in medium with 0–9 % (w/v) NaCl. The cell-wall peptidoglycan was of the A1α type, with l-lysine as the diagnostic diamino acid. The major respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C15 : 0 (45.4 %), iso-C15 : 0 (22.0 %) and anteiso-C17 : 0 (11.2 %). The genomic DNA G+C content was about 39.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P9T was most closely related to Jeotgalibacillus campisalis DSM 18983T (96.8 %) and Jeotgalibacillus marinus DSM 1297T (96.5 %). These two recognized species formed a coherent cluster with strain P9T that was supported by a bootstrap value of 99 %. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, strain P9T ( = DSM 23228T = LMG 25523T) represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus soli sp. nov. is proposed.

scholarly journals Larkinella bovis sp. nov., isolated from fermented bovine products, and emended descriptions of the genus Larkinella and of Larkinella insperata Vancanneyt et al. 2006

2011 ◽  
Vol 61 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Rangasamy Anandham ◽  
Soon-Wo Kwon ◽  
Hang-Yeon Weon ◽  
Soo-Jin Kim ◽  
Yi-Seul Kim ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
Major Respiratory Quinone ◽  
Motile By Gliding

A novel bacterial strain, designated M2T2B15T, was isolated from fermented bovine products and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Cells were strictly aerobic, Gram-reaction-negative, oxidase- and catalase-positive rods that lacked flagella and were motile by gliding. Flexirubin-type pigments were absent. 16S rRNA gene sequence analysis indicated that strain M2T2B15T was related most closely to Larkinella insperata LMG 22510T (94.4 % similarity) but shared <87 % similarity with other members of the phylum Bacteroidetes. The major cellular fatty acids were C16 : 1 ω5c, iso-C15 : 0 and iso-C17 : 0 3-OH. The polar lipids were phosphatidylethanolamine, phosphatidylserine, two unidentified aminophospholipids and two unidentified polar lipids. Menaquinone 7 (MK-7) was the major respiratory quinone. The G+C content of the DNA of strain M2T2B15T was 52 mol%. The phenotypic, genotypic and phylogenetic data presented clearly indicate that strain M2T2B15T represents a novel species of the genus Larkinella, for which the name Larkinella bovis sp. nov. is proposed. The type strain is M2T2B15T (=KACC 14040T =NBRC 106324T). Emended descriptions of the genus Larkinella and of Larkinella insperata Vancanneyt et al. 2006 are also proposed.

scholarly journals Deinococcus citri sp. nov., isolated from citrus leaf canker lesions

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4134-4140 ◽  
Author(s):  
Iftikhar Ahmed ◽  
Saira Abbas ◽  
Takuji Kudo ◽  
Muhammad Iqbal ◽  
Toru Fujiwara ◽  
...  
Keyword(s):  
Type Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Content Type ◽  
Citrus Leaf ◽  
Link Type ◽  
Physiological And Biochemical Characteristics ◽  
Gene Sequence Analysis

A Gram-stain-positive, strictly aerobic, non-motile, coccoid bacterium, designated NCCP-154T, was isolated from citrus leaf canker lesions and was subjected to a polyphasic taxonomic study. Strain NCCP-154T grew at 10–37 °C (optimum 30 °C) and at pH 7.0–8.0 (optimum pH 7.0). The novel strain exhibited tolerance of UV irradiation (>1000 J m−2). Based on 16S rRNA gene sequence analysis, strain NCCP-154T showed the highest similarity to Deinococcus gobiensis CGMCC 1.7299T (98.8 %), and less than 94 % similarity to other closely related taxa. The chemotaxonomic data [major menaquinone, MK-8; cell-wall peptidoglycan type, A3β (Orn–Gly2); major fatty acids, summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH; 35.3 %) followed by C16 : 0 (12.7 %), iso-C17 : 1ω9c (9.2 %), C17 : 1ω8c (7.4 %) and iso-C17 : 0 (6.9 %); major polar lipids made up of several unidentified phosphoglycolipids and glycolipids and an aminophospholipid, and mannose as the predominant whole-cell sugar] also supported the affiliation of strain NCCP-154T to the genus Deinococcus . The level of DNA–DNA relatedness between strain NCCP-154T and D. gobiensis JCM 16679T was 63.3±3.7 %. The DNA G+C content of strain NCCP-154T was 70.0 mol%. Based on the phylogenetic analyses, DNA–DNA hybridization and physiological and biochemical characteristics, strain NCCP-154T can be differentiated from species with validly published names. Therefore, it represents a novel species of the genus Deinococcus . The name Deinococcus citri sp. nov. is proposed, with the type strain NCCP-154T ( = JCM 19024T = DSM 24791T = KCTC 13793T).

scholarly journals Hydrotalea sandarakina sp. nov., isolated from a hot spring runoff, and emended descriptions of the genus Hydrotalea and the species Hydrotalea flava

2012 ◽  
Vol 62 (Pt_7) ◽  
pp. 1603-1608 ◽  
Author(s):  
Luciana Albuquerque ◽  
Fred A. Rainey ◽  
M. Fernanda Nobre ◽  
Milton S. da Costa
Keyword(s):  
Type Strain ◽  
Type Species ◽  
Hot Spring ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Optimum Growth Temperature ◽  
Link Type ◽  
Spring Runoff ◽  
Physiological And Biochemical Characteristics

Two bacterial isolates, designated AF-51T and AF-50, with an optimum growth temperature of about 45 °C and an optimum pH for growth between 6.0 and 6.5, were recovered from a hot spring in the Furnas, Área da Fonte 1825, on the Island of São Miguel in the Azores. Based on 16S rRNA gene sequence analysis, these strains were related most closely to the type strain of Hydrotalea flava at a pairwise similarity of 95.7 %. The two strains were orange-pigmented and formed non-motile, rod-shaped cells that stained Gram-negative and were aerobic and oxidase- and catalase-positive. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C16 : 0. The major respiratory quinone was menaquinone 7. Based on phylogenetic, physiological and biochemical characteristics, these strains from the Azores are considered to represent a single novel species of the genus Hydrotalea , for which the name Hydrotalea sandarakina sp. nov. is proposed. The type strain is AF-51T ( = DSM 23241T = LMG 25526T). We provide emended descriptions of the genus Hydrotalea and of H. flava to reflect new results obtained in this study.

Fulvitalea axinellae gen. nov., sp. nov., a member of the family Flammeovirgaceae isolated from the Mediterranean sponge Axinella verrucosa

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1678-1683 ◽  
Author(s):  
Markus Haber ◽  
Sigal Shefer ◽  
Assunta Giordano ◽  
Pierangelo Orlando ◽  
Agata Gambacorta ◽  
...  
Keyword(s):  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Novel Genus And Species ◽  
Major Respiratory Quinone ◽  
The Mediterranean

The yellow-pigmented, non-motile, Gram-negative, strictly aerobic, rod-shaped bacterial strain VI.18T was isolated from the Mediterranean sponge Axinella verrucosa collected off the coast near Sdot Yam, Israel. Results from 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flammeovirgaceae . The highest nucleotide similarity (91.4 %) occurred with Aureibacter tunicatorum A5Q-118T. The predominant cellular fatty acids of strain VI.18T were iso-C15 : 0 (56.0 %), iso-C17 : 1ω9c (22.8 %) and C16 : 0 (7.4 %) and its major respiratory quinone was MK-7. The DNA G+C content was 47.5 mol%. The strain could readily be distinguished from its phylogenetically closest relatives by phenotypic, physiological and chemotaxonomic properties. On the basis of the data from the present polyphasic study, we propose a novel genus and species within the family Flammeovirgaceae , with the name Fulvitalea axinellae gen. nov., sp. nov. Strain VI.18T ( = ATCC BAA-2395T  = LMG 26722T) is the type strain of Fulvitalea axinellae.

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