scholarly journals Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4792-4799 ◽  
Author(s):  
Jae-Chan Lee ◽  
Young-Sook Kim ◽  
Bong-Sik Yun ◽  
Kyung-Sook Whang
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polyphasic Analysis ◽  
Optimum Ph ◽  
Unknown Phospholipid

A Gram-stain-negative, halotolerant and alkalitolerant bacterium, designated strain BH103T, was isolated from saltern soil in Gomso, Korea. Cells of strain BH103T were strictly aerobic, motile, straight rods and grew at pH 7.0–10.8 (optimum, pH 8.5), at 10–55 °C (optimum, 28 °C) and at salinities of 0–23 % (w/v) NaCl (optimum, 14 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH103T belongs to the genus Halomonas, showing highest sequence similarity to Halomonas boliviensis LC1T (97.7 %), Halomonas neptunia Eplume1T (97.7 %), Halomonas variabilis IIIT (97.7 %), Halomonas alkaliantarctica CRSST (97.7 %), Halomonas olivaria TYRC17T (97.5 %), Halomonas titanicae BH1T (97.2 %) and Halomonas sulfidaeris Esulfide1T (96.2 %). The predominant ubiquinone was Q-9. The major fatty acids were C18 : 1ω7c, C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C12 : 0 3-OH. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and an unknown phospholipid. The DNA G+C content of this novel isolate was 54.7 mol%. DNA–DNA relatedness between strain BH103T and H. boliviensis KACC 16615T, H. neptunia KCTC 2888T, H. variabilis KCTC 2889T, H. alkaliantarctica KCTC 22844T, H. olivaria DSM 19074T, H. titanicae JCM 16411T and H. sulfidaeris DSM 15722T was 45, 41, 39, 32, 38, 45 and 35 %, respectively. On the basis of polyphasic analysis from this study, strain BH103T represents a novel species of the genus Halomonas, for which the name Halomonas salicampi sp. nov. is proposed. The type strain is BH103T ( = KACC 17609T = NBRC 109914T = NCAIM B 02528T).

scholarly journals Bacillus taiwanensis sp. nov., isolated from a soil sample from Taiwan

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2078-2084 ◽  
Author(s):  
Bo Liu ◽  
Guo-Hong Liu ◽  
Cetin Sengonca ◽  
Peter Schumann ◽  
Ming-Kuang Wang ◽  
...  
Keyword(s):  
Soil Sample ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Optimum Ph ◽  
Diamino Acid

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium (FJAT-14571T) was isolated from a soil sample in Taiwan. Strain FJAT-14571T grew at 20–40 °C (optimum 35 °C), pH 6–10 (optimum pH 8) and 0–2 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FJAT-14571T was a member of the genus Bacillus and was most closely related to Bacillus oceanisediminis DSM 24771T (96.2 %). DNA–DNA relatedness between strain FJAT-14571T and B. oceanisediminis DSM 24771T was low (32.0 % ± 0.88 %). The diagnostic diamino acid of the peptidoglycan of strain FJAT-14571T was meso-diaminopimelic acid and the predominant menaquinone was MK-7 (96.6 %). The major cellular fatty acids were iso-C15 : 0 (46.4 %), anteiso-C15 : 0 (7.6 %), iso-C17 : 0 (8.2 %) and iso-C16 : 0 (10.0 %) and the DNA G+C content was 40.8 mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that strain FJAT-14571T represents a novel species within the genus Bacillus, for which the name Bacillus taiwanensis sp. nov. is proposed. The type strain is FJAT-14571T ( = DSM 27845T = CGMCC1.1 2698T).

scholarly journals Aquabacterium limnoticum sp. nov., isolated from a freshwater spring

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 698-704 ◽  
Author(s):  
Wen-Ming Chen ◽  
Nian-Tsz Cho ◽  
Shwu-Harn Yang ◽  
A. B. Arun ◽  
Chiu-Chung Young ◽  
...  
Keyword(s):  
Hydroxy Fatty Acid ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Polar Lipid Profile ◽  
Optimum Ph ◽  
Freshwater Spring

A Gram-negative, facultatively anaerobic, short-rod-shaped, non-motile and non-spore-forming bacterial strain, designated ABP-4T, was isolated from a freshwater spring in Taiwan and was characterized using the polyphasic taxonomy approach. Growth occurred at 20–40 °C (optimum, 30–37 °C), at pH 7.0–10.0 (optimum, pH 7.0–9.0) and with 0–3 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ABP-4T, together with Aquabacterium fontiphilum CS-6T (96.4 % sequence similarity), Aquabacterium commune B8T (96.1 %), Aquabacterium citratiphilum B4T (95.5 %) and Aquabacterium parvum B6T (94.7 %), formed a deep line within the order Burkholderiales. Strain ABP-4T contained summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c), C18 : 1 ω7c and C16 : 0 as predominant fatty acids. The major cellular hydroxy fatty acid was C10 : 0 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 68.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and several uncharacterized phospholipids. The DNA–DNA relatedness of strain ABP-4T with respect to recognized species of the genus Aquabacterium was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain ABP-4T represents a novel species in the genus Aquabacterium, for which the name Aquabacterium limnoticum sp. nov. is proposed. The type strain is ABP-4T ( = BCRC 80167T = KCTC 23306T).

scholarly journals Zhihengliuella somnathii sp. nov., a halotolerant actinobacterium from the rhizosphere of a halophyte Salicornia brachiata

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3137-3142 ◽  
Author(s):  
Bhavanath Jha ◽  
Vijay Kumar Singh ◽  
Angelo Weiss ◽  
Anton Hartmann ◽  
Michael Schmid
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Salicornia Brachiata ◽  
A Value ◽  
Sequence Similarities

Two novel, Gram-stain-positive, rod-shaped, halotolerent bacteria, strains JG 03T and JG 05 were isolated from the rhizosphere of Salicornia brachiata, an extreme halophyte. Comparative analyses of 16S rRNA gene sequences showed that they were closely related to members of the genus Zhihengliuella, with sequence similarities of 96.9–99.1 %. The sequence similarity of strains JG 03T and JG 05 with each other was 99.4 %. DNA–DNA hybridization of JG 03T and JG 05 with other species of the genus Zhihengliuella with validly published names showed reassociation values of 19.8 %–53.4 % and a value of 91.4 % between each other. The peptidoglycan type of both strains was A4α and MK-9 and MK-10 were the predominant menaquinones. The predominant fatty acid in JG 03T was anteiso-C15 : 0 and anteiso-C17 : 0. However, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids in strain JG 05. The DNA G+C content of strains JG 03T and JG 05 was 70.0 and 70.1 mol%, respectively. In nutrient broth medium both strains grew at NaCl concentrations of up to 15 % (w/v). On the basis of chemotaxonomic characteristics and phylogenetic analyses, strains JG 03T and JG 05 should be affiliated to the genus Zhihengliuella. Strains JG 03T and JG 05 represent a novel species of the genus Zhihengliuella for which the name Zhihengliuella somnathii sp. nov. is proposed. The type strain is JG 03T ( = DSM 23187T = IMCC 253T).

scholarly journals Novosphingobium ovatum sp. nov., isolated from a freshwater mesocosm

2020 ◽  
Vol 70 (10) ◽  
pp. 5243-5254 ◽  
Author(s):  
Wen-Ming Chen ◽  
Cheng-Ye Cai ◽  
Der-Shyan Sheu ◽  
Jyh-Ming Tsai ◽  
Shih-Yi Sheu
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph

A bacterial strain, designated FSY-8T, was isolated from a freshwater mesocosm in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain FSY-8T were aerobic, Gram-stain-negative, rod-shaped, non-motile and formed yellow coloured colonies on Reasoner's 2A agar. Growth occurred at 20–40 °C (optimum, 30–37 °C) and pH 5–7 (optimum, pH 6) and in the presence of 0–0.5 % NaCl (optimum, 0 %, w/v). The major fatty acids (>10 %) of strain FSY-8T were summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c) and summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, diphosphatidylglycerol, an uncharacterized aminophospholipid, an uncharacterized glycolipid and an uncharacterized lipid. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 64.8 mol %. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain FSY-8T formed a phylogenetic lineage in the genus Novosphingobium . Strain FSY-8T showed 71.6–77.2 % average nucleotide identity and 19.9–22.8 % digital DNA–DNA hybridization identity with the strains of other Novosphingobium species. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain FSY-8T should be classified in a novel species of the genus Novosphingobium , for which the name Novosphingobium ovatum sp. nov. is proposed. The type strain is FSY-8T (=BCRC 81051T=LMG 30053T=KCTC 52812T).

scholarly journals Muriicola marianensis sp. nov., isolated from seawater

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 407-411 ◽  
Author(s):  
Jing Hu ◽  
Wei-Yan Zhang ◽  
Xin-Qi Zhang ◽  
Hong-Cheng ◽  
Xu-Fen Zhu ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph

A Gram-stain-negative, aerobic, orange-pigmented, rod-shaped and non-motile bacterium, designated strain A6B8T, was isolated from seawater of the Mariana Trench. The isolate grew at 4–50 °C (optimum 30–35 °C), at pH 6.5–8.0 (optimum pH 7.5) and with 0.5–4.0 % (w/v) NaCl (optimum 1.0–2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A6B8T was related most closely to the genus Muriicola and shared highest sequence similarity of 97.7 % with Muriicola jejuensis EM44T. Chemotaxonomic analysis showed menaquinone 6 (MK-6) was the predominant isoprenoid and iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH were the major cellular fatty acids. The polar lipid profile of strain A6B8T included phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 47.1 mol%. The DNA–DNA relatedness value (23.3 %) clearly demonstrated that strains A6B8T and M. jejuensis EM44T were representatives of two different species. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, A6B8T ( = CGMCC 1.12606T = KCTC 32436T) is considered to be the type strain of a novel species of the genus Muriicola , for which the name Muriicola marianensis sp. nov. is proposed.

scholarly journals Chitinophaga ginsengihumi sp. nov., isolated from soil of ginseng rhizosphere

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2599-2604 ◽  
Author(s):  
Jae-Chan Lee ◽  
Kyung-Sook Whang
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Polyphasic Analysis ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Optimum Ph

A novel strain designated SR18T was isolated from the rhizosphere soil of a ginseng in Korea. Cells were Gram-staining-negative, motile by gliding, catalase-positive and oxidase-negative, non-spore-forming rods. The isolate grew aerobically at 15–45 °C (optimum 28 °C), pH 5.5–7.5 (optimum pH 7.0) and with 0–3.0 % (w/v) NaCl (optimum 1.5 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SR18T belongs to the genus Chitinophaga with sequence similarity of 97.2 % and 97.0 % to Chitinophaga japonensis 758T and Chitinophaga rupis CS5-B1T, respectively. Similarity to other species of the genus Chitinophaga was 92.8–95.5 %. The predominant menaquinone was MK-7. Major fatty acids were iso-C15 : 0 and C16 : 1ω5c. The polar lipids included phosphatidylethanolamine, unidentified phospholipids, unknown aminolipids and unknown lipids. The genomic DNA G+C content was 45.3 mol%. DNA–DNA relatedness between strain SR18T and C. japonensis NBRC 16041T was 29–32 %. On the basis of polyphasic analysis from this study, strain SR18T represents a novel species of the genus Chitinophaga , for which the name Chitinophaga ginsengihumi sp. nov. is proposed. The type strain is SR18T ( = KACC 17604T = NBRC 109832T).

scholarly journals Belliella marina sp. nov., isolated from seawater

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4353-4357 ◽  
Author(s):  
Lei Song ◽  
Hongcan Liu ◽  
Jian Wang ◽  
Ying Huang ◽  
Xin Dai ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Optimum Ph ◽  
The Indian Ocean ◽  
Bacterium Strain

A Gram-stain-negative, rod-shaped bacterium, strain SW112T, was isolated from a seawater sample collected from the Indian Ocean. The strain was strictly aerobic and catalase- and oxidase-positive. Strain SW112T grew at 4–42 °C (optimum 30 °C), at pH 5.5–9.5 (optimum pH 7.5) and in the presence of 0–9.0 % (w/v) NaCl (optimum 2.0–3.0 %). The predominant cellular fatty acids were iso-C15 : 0 (29.7 %), iso-C17 : 03-OH (14.3 %) and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 15.1 %). The major menaquinone was menaquinone-7 and the major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of strain SW112T was 39 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SW112T was related to members of the genus Belliella, showing the highest similarity with Belliella aquatica TS-T86Tand Belliella baltica DSM 15883T (96.5 % and 96.4 % sequence similarity, respectively). On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that strain SW112T represents a novel species of the genus Belliella, for which the name Belliella marina sp. nov. is proposed. The type strain is SW112T ( = CGMCC 1.15180T = KCTC 33694T).

scholarly journals Hydrocarboniphaga daqingensis sp. nov., isolated from a freshwater lake

2011 ◽  
Vol 61 (2) ◽  
pp. 408-411 ◽  
Author(s):  
Ying Liu ◽  
Xue-Feng Song ◽  
Jia-Tong Jiang ◽  
Ying-Hao Liu ◽  
Cheng-Jun Xu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
North East ◽  
Optimum Ph

A Gram-negative bacterium, designated B2-9T, was isolated from water of a slightly alkaline lake, Longhu Lake in Daqing, north-east China. Strain B2-9T was an aerobic rod, heterotrophic, catalase- and oxidase-positive and motile by means of a single polar flflagellum. The isolate grew well on n-alkanes C9–C17 and weakly on C6–C8, C18 and C19. Growth occurred at 10–37 °C (optimum 20–25 °C), at pH 5.5–9.5 (optimum pH 7.0) and with ≤1.0 % (w/v) NaCl. The major fatty acids were C18 : 1 ω7c (41.4 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c; 18.5 %) and C16 : 0 (10.9 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain B2-9T belonged to the genus Hydrocarboniphaga of the class Gammaproteobacteria and shared 97.0 % 16S rRNA gene sequence similarity with Hydrocarboniphaga effusa AP103T. DNA–DNA relatedness between strain B2-9T and H. effusa DSM 16095T was 24 %. The DNA G+C content of strain B2-9T was 66 mol%. Strain B2-9T is considered to represent a novel species of the genus Hydrocarboniphaga, for which the name Hydrocarboniphaga daqingensis sp. nov. is proposed. The type strain is B2-9T (=CGMCC 1.7049T =NBRC 104238T).

scholarly journals Flavobacterium banpakuense sp. nov., isolated from leaf-and-branch compost

2011 ◽  
Vol 61 (7) ◽  
pp. 1595-1600 ◽  
Author(s):  
Joong-Jae Kim ◽  
Hyun Mi Jin ◽  
Hyo Jung Lee ◽  
Che Ok Jeon ◽  
Eiko Kanaya ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Cellular Fatty Acids ◽  
The Family ◽  
Optimum Ph ◽  
Motile By Gliding

A strictly aerobic, Gram-stain-negative, yellow-pigmented, non-spore-forming, motile (by gliding), rod-shaped bacterium, designated strain 15F3T, was isolated from leaf-and-branch compost. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 15F3T was most closely related to Flavobacterium reichenbachii WB 3.2-61T and formed a distinct phyletic lineage within the genus Flavobacterium, the type genus of the family Flavobacteriaceae. Growth was observed at 10–34 °C (optimum, 30 °C) and pH 6.0–8.0 (optimum, pH 7.0). No growth occurred in the presence of ≥2 % (w/v) NaCl. Strain 15F3T reduced nitrate to nitrogen and showed catalase activity but no oxidase activity. The predominant cellular fatty acids were iso-C15 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major isoprenoid quinone was menaquinone-6. The G+C content of the genomic DNA was 31.1 mol%. On the basis of data from this polyphasic study, strain 15F3T may be classified as a representative of a novel species within the genus Flavobacterium, for which the name Flavobacterium banpakuense sp. nov. is proposed; the type strain is 15F3T ( = KACC 14225T  = JCM 16466T).

scholarly journals Phaeocystidibacter marisrubri sp. nov., a member of the family Cryomorphaceae isolated from Red Sea sediment

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2199-2203 ◽  
Author(s):  
Xiaowei Zheng ◽  
Hongcan Liu ◽  
Lei Song ◽  
Limin Zhang ◽  
Haina Wang ◽  
...  
Keyword(s):  
Red Sea ◽  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
The Family ◽  
Optimum Ph

Strain G18T, a Gram-stain-negative, aerobic, rod-shaped, motile, non-fermentative, yellow-pigmented bacterium, was isolated from Red Sea sediment. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain G18T was most closely related to Phaeocystidibacter luteus PG2S01T with 95.3 % similarity. Growth of strain G18T occurred at 10–42 °C (optimum 28–37 °C), pH 5.0–9.0 (optimum pH 6.0–8.0) and in the presence of 0.5–10 % NaCl (optimum 2–5 %). The major fatty acids of strain G18T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 1 G and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, unidentified aminolipids, phospholipids and other lipids. The predominant quinone was menaquinone 6 (MK-6). The G+C content of the genomic DNA from strain G18T was 39.0 mol%. Based on the phylogenetic and phenotypic properties, strain G18T represents a novel species of the genus Phaeocystidibacter, for which the name Phaeocystidibacter marisrubri sp. nov. is proposed. The type strain is G18T ( = CGMCC 1.14954T = JCM 30614T).

Sign in / Sign up

Export Citation Format

Share Document