Evaluation of two multiplex real-time PCR screening capabilities for the detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis in blood samples generated from murine infection models

2012 ◽  
Vol 61 (11) ◽  
pp. 1546-1555 ◽  
Author(s):  
Simon A. Weller ◽  
Victoria Cox ◽  
Angela Essex-Lopresti ◽  
Margaret G. Hartley ◽  
Tanya M. Parsons ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Real Time ◽  
Francisella Tularensis ◽  
Real Time Pcr ◽  
Blood Samples ◽  
Pcr Screening ◽  
Infection Models

Monitoring biothreat agents (Francisella tularensis, Bacillus anthracis and Yersinia pestis) with a portable real-time PCR instrument

2015 ◽  
Vol 115 ◽  
pp. 89-93 ◽  
Author(s):  
Markos Mölsä ◽  
Heidi Hemmilä ◽  
Anna Katz ◽  
Jukka Niemimaa ◽  
Kristian M. Forbes ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Real Time ◽  
Francisella Tularensis ◽  
Real Time Pcr

Simultaneous real-time PCR detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis

2007 ◽  
Vol 26 (3) ◽  
pp. 207-211 ◽  
Author(s):  
T. Skottman ◽  
H. Piiparinen ◽  
H. Hyytiäinen ◽  
V. Myllys ◽  
M. Skurnik ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Real Time ◽  
Francisella Tularensis ◽  
Real Time Pcr ◽  
Pcr Detection

scholarly journals Rapid Antibiotic Susceptibility Testing of Tier-1 Agents Bacillus anthracis, Yersinia pestis, and Francisella tularensis Directly From Whole Blood Samples

2021 ◽  
Vol 12 ◽  
Author(s):  
Shahar Rotem ◽  
Ohad Shifman ◽  
Moshe Aftalion ◽  
David Gur ◽  
Tamar Aminov ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Francisella Tularensis ◽  
Whole Blood ◽  
Antibiotic Susceptibility ◽  
Blood Samples ◽  
Wide Range ◽  
Tier 1 ◽  
Whole Blood Samples ◽  
Susceptibility Tests

Rapid antibiotic susceptibility tests, performed directly on whole blood samples, will offer great clinical advantages. This issue is of considerable importance when it comes to bioterror pathogens where prompt antibiotic treatment should be offered to infected patients as well as prophylaxis to suspected exposed individuals. Herein, we describe a novel and rapid method, named MAPt, that is based on the direct application of a blood sample onto solid agar that has been embedded with different concentrations of the tested antibiotic. Following a short incubation, bacterial growth is monitored by qPCR. The method was applied on blood cultures and whole blood samples inoculated with the Tier-1 pathogens Bacillus anthracis, Yersinia pestis, and Francisella tularensis. The use of agar medium, which better supports the growth of bacteria at low concentrations, together with the use of qPCR, which provides sensitivity and specificity, allowed minimal inhibitory concentration (MIC) determination to a wide range of bacterial concentrations, ranging from ∼5 × 102 cfu/ml up to 108 cfu/ml. The omission of the enrichment procedure in blood culture and the isolation step, both required in standard antibiotic susceptibility tests (ASTs), allowed a dramatic reduction in time to answer, from a few days to a few hours. The total time required for MIC determination was ∼6 h for fast-growing bacteria, such as B. anthracis, and 12–16 h for slow-growing bacteria, represented by Y. pestis and F. tularensis. Accordingly, MAPt may offer health authorities means for public preparedness in the case of a bioterror attack as well as prompt clinical treatment options in common blood stream infections.

scholarly journals A two minute liquid based sample preparation for rapid SARS-CoV2 real-time PCR screening: a multicentre evaluation

2020 ◽  
pp. 104720
Author(s):  
Eric C.J. Claas ◽  
Pieter W. Smit ◽  
Mario J.A.W.M. van Bussel ◽  
Harold Verbakel ◽  
Mohammed Taouil ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Screening

Recovery of Francisella tularensis from soil samples by filtration and detection by real-time PCR and cELISA

2008 ◽  
Vol 10 (3) ◽  
pp. 362 ◽  
Author(s):  
Ricela Sellek ◽  
Oscar Jimenez ◽  
Carmen Aizpurua ◽  
Begoña Fernandez-Frutos ◽  
Patricia De Leon ◽  
...  
Keyword(s):  
Real Time ◽  
Francisella Tularensis ◽  
Real Time Pcr ◽  
Soil Samples

scholarly journals Detection of Yersinia pestis in Sputum by Real-Time PCR

2003 ◽  
Vol 41 (10) ◽  
pp. 4873-4875 ◽  
Author(s):  
C. Loiez ◽  
S. Herwegh ◽  
F. Wallet ◽  
S. Armand ◽  
F. Guinet ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Real Time ◽  
Real Time Pcr
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