Detection of Borrelia burgdorferi in patient’s blood samples using real time PCR and its countertype in nested version- comparison of methods

Introduction: Invasive aspergillosis is a severe complication of cytotoxic chemotherapies and bone marrow transplantation (BMT). The aim of this study was to assess the utility of a real-time PCR assay for the early diagnosis of Aspergillus species in blood samples from BMT patients. Methodology: Blood specimens (n = 993) from patients (n = 82) scheduled for BMT were collected prior to transplant and for 100 days post transplantation. The specimens were later tested using an Aspergillus-specific real-time PCR assay. Cultures of clinical samples, along with sonography and computerized tomographic scans, were performed as standard of care. Results: Aspergillus DNA was positive in 94 sequential blood samples from 13 patients with clinical and radiological signs of infection. Samples from three of these patients were PCR-positive for Aspergillus in the first week of admission, prior to transplantation. Four patients with aspergillosis were cured with antifungal agents and nine died. An additional 12 patients without clinical signs of infection were PCR-positive on one occasion each, while two patients with clinical signs of infection were PCR-negative. Compared to routine methods of aspergillosis diagnosis, the respective sensitivity, specificity, negative, and positive predictive values of the PCR method by patient were 86.6%, 82%, 96.5% and 52%. Conclusions: The results show that Aspergillus infections in the blood of bone marrow transplant patients can be dectected by PCR methods. Early detection of Aspergillus infections by PCR has the potential to positively impact patient mortality rate and provide cost savings to hospitals.

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Stability of hepatitis C virus RNA in blood samples by TaqMan real-time PCR

Journal of Clinical Laboratory Analysis ◽

10.1002/jcla.20354 ◽

2010 ◽

Vol 24 (3) ◽

pp. 134-138 ◽

Cited By ~ 5

Author(s):

Kenan Sener ◽

Mehmet Yapar ◽

Orhan Bedir ◽

Cem Gül ◽

Ömer Coskun ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Real Time ◽

Real Time Pcr ◽

Hepatitis C Virus Rna ◽

Blood Samples ◽

Virus Rna

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Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Journal of Veterinary Research ◽

10.1515/jvetres-2016-0002 ◽

2016 ◽

Vol 60 (1) ◽

pp. 7-12 ◽

Cited By ~ 2

Author(s):

Aliasghar Bahari ◽

Masoud Sabouri Ghannad ◽

Omid Dezfoulian ◽

Fereydon Rezazadeh ◽

Ali Sadeghi-Nasab

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Mediastinal Lymph Node ◽

Pulmonary Adenocarcinoma ◽

Ovine Pulmonary Adenocarcinoma ◽

Jaagsiekte Sheep Retrovirus ◽

Tissue Samples ◽

Blood Samples ◽

Proviral Dna ◽

Healthy Sheep

Abstract Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.

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