scholarly journals A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system

2013 ◽  
Vol 62 (5) ◽  
pp. 773-777 ◽  
Author(s):  
Guy Prod’hom ◽  
Christian Durussel ◽  
Gilbert Greub
Keyword(s):  
Ammonium Chloride ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Blood Cultures ◽  
Correct Identification ◽  
Antibiotic Susceptibility Testing ◽  
Bacterial Identification ◽  
Lower Accuracy ◽  
Direct Inoculation ◽  
Vitek 2

An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99 % for Enterobacteriaceae and 74 % for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 and 0.3 % for Enterobacteriaceae, and 0.7 and 0.1 % for staphylococci, respectively. Thus, bacterial pellets prepared with ammonium chloride allow direct inoculation of VITEK cards with excellent accuracy for Enterobacteriaceae and a lower accuracy for staphylococci.

scholarly journals Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

2017 ◽  
Vol 22 (6) ◽  
pp. 585-608 ◽  
Author(s):  
Yiyan Li ◽  
Xing Yang ◽  
Weian Zhao
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Point Of Care ◽  
Single Cells ◽  
Turnaround Time ◽  
Resistant Bacteria ◽  
Representative Point ◽  
Antibiotic Susceptibility Testing ◽  
Bacterial Identification ◽  
Automated Systems

Rapid bacterial identification (ID) and antibiotic susceptibility testing (AST) are in great demand due to the rise of drug-resistant bacteria. Conventional culture-based AST methods suffer from a long turnaround time. By necessity, physicians often have to treat patients empirically with antibiotics, which has led to an inappropriate use of antibiotics, an elevated mortality rate and healthcare costs, and antibiotic resistance. Recent advances in miniaturization and automation provide promising solutions for rapid bacterial ID/AST profiling, which will potentially make a significant impact in the clinical management of infectious diseases and antibiotic stewardship in the coming years. In this review, we summarize and analyze representative emerging micro- and nanotechnologies, as well as automated systems for bacterial ID/AST, including both phenotypic (e.g., microfluidic-based bacterial culture, and digital imaging of single cells) and molecular (e.g., multiplex PCR, hybridization probes, nanoparticles, synthetic biology tools, mass spectrometry, and sequencing technologies) methods. We also discuss representative point-of-care (POC) systems that integrate sample processing, fluid handling, and detection for rapid bacterial ID/AST. Finally, we highlight major remaining challenges and discuss potential future endeavors toward improving clinical outcomes with rapid bacterial ID/AST technologies.

scholarly journals Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0205603 ◽  
Author(s):  
Marlène Sauget ◽  
Xavier Bertrand ◽  
Didier Hocquet
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Blood Cultures ◽  
Antibiotic Susceptibility Testing ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals 144. Organism Identification and Antibiotic Susceptibilities with Verigene Blood Culture Assay: a Retrospective Single-Center Study

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S99-S100
Author(s):  
Stephanie Tchen ◽  
Steven Smoke ◽  
Maria DeVivo
Keyword(s):  
Blood Culture ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Medical Center ◽  
Academic Medical Center ◽  
Blood Cultures ◽  
Molecular Testing ◽  
Antibiotic Susceptibility Testing ◽  
Resistance Determinants ◽  
Organism Identification

Abstract Background The Verigene blood culture assay is a rapid molecular testing platform for positive blood cultures. Verigene detects a limited number of bacteria and a limited number of antibiotic resistance determinants. While certain Verigene results have clear implications for optimal antibiotic therapy prior to complete antibiotic susceptibility testing, others do not. The purpose of this study was to compare the results of the Verigene blood culture assay with standard organism identification and antibiotic susceptibility testing. Methods This was a retrospective cohort study conducted at a single academic medical center. The study period was 14 months from November 2017 to December 2018. All Verigene results from the study period were reviewed and compared with the results of standard organism identification and susceptibility testing. Organism identification and antibiotic susceptibility testing were performed by Vitek MS and Vitek 2. Duplicate results from the same patient were excluded. The primary outcome was the percentage of blood cultures correctly identified by Verigene. Secondary outcomes included the antibiotic susceptibility of organisms identified by Verigene in the presence and absence of resistance determinants and the identity and frequency of organisms not detected by Verigene. Results A total of 782 Verigene results were screened. After exclusions, 675 Verigene results including 737 organisms from 597 patients were included. Of 737 organisms, Verigene correctly identified 611 (82.9%), incorrectly identified 19 (2.6%) and was unable to identify 107 (14.5%) off-panel organisms. Tables 1 and 2 outline the antibiotic susceptibility of organisms by the presence or absence of resistance determinants in Gram-negative and Gram-positive bacteria, respectively. Table 3 describes the identities of the organisms not detected by Verigene, stratified by Gram stain result. Conclusion The Verigene blood culture assay demonstrated accuracy in identifying organisms and predicting antibiotic susceptibility. These results will help inform the prospective interpretation of Verigene results and subsequent antibiotic selection at the study institution. Disclosures All authors: No reported disclosures.

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