Isolation and characterization of provisional serovar Shigella boydii E16553 from diarrhoeal patients in Bangladesh

2005 ◽  
Vol 54 (5) ◽  
pp. 477-480 ◽  
Author(s):  
M Ansaruzzaman ◽  
Marzia Sultana ◽  
Kaisar A Talukder ◽  
K Alam ◽  
S Matsushita ◽  
...  
Keyword(s):  
Type Strain ◽  
Shigella Dysenteriae ◽  
Pfge Type ◽  
Common Source ◽  
Reference Laboratory ◽  
Japanese Strain ◽  
Shigella Boydii ◽  
Virulence Markers ◽  
Isolation And Characterization

In previous studies with strains of the Shigella dysenteriae provisional serovars E22383 and E23507 from diarrhoeal stools from patients in Bangladesh, two strains of Shigella species were identified as Shigella boydii provisional serovar E16553 by a reference laboratory. Further tests with an antiserum to an international type strain of the provisional serovar E16553 identified an additional 15 isolates. None of the isolates reacted with antisera to the established Shigella serovars or any other provisional serovars reported so far and all showed biochemical reactions typical of S. boydii. All of the isolates harboured the 140 MDa invasion plasmid, had the ipaH gene and produced keratoconjunctivitis in the guinea pig eye. All isolates were susceptible to ampicillin, sulfamethoxazole-trimethoprim, nalidixic acid, ciprofloxacin and mecillinam but eight strains were resistant to tetracycline. A single PFGE type (type A) was shown for all 17 clinical isolates, indicating a common source of origin. The pulsotype of the Bangladeshi isolates was closely related to that of a Japanese strain but was different from that of the type strain. On the basis of these biochemical, serological and virulence markers, and diverse geographical origin, it is recommended that the provisional status of serovar E16553 be changed and that it be included in the international serotyping classification scheme as S. boydii 19.

scholarly journals Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

2020 ◽  
Vol 21 (9) ◽  
pp. 3170
Author(s):  
Mikhail M. Shneider ◽  
Anna A. Lukianova ◽  
Peter V. Evseev ◽  
Anna M. Shpirt ◽  
Marsel R. Kabilov ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Negative Impact ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Narrow Host Range ◽  
Isolation And Characterization ◽  
Control Application ◽  
Hydrolytic Mechanism

Phytopathogenic bacteria belonging to the Pectobacterium and Dickeya genera (soft-rot Pectobacteriaceae) are in the focus of agriculture-related microbiology because of their diversity, their substantial negative impact on the production of potatoes and vegetables, and the prospects of bacteriophage applications for disease control. Because of numerous amendments in the taxonomy of P. carotovorum, there are still a few studied sequenced strains among this species. The present work reports on the isolation and characterization of the phage infectious to the type strain of P. carotovorum. The phage Arno 160 is a lytic Podovirus representing a potential new genus of the subfamily Autographivirinae. It recognizes O-polysaccahride of the host strain and depolymerizes it in the process of infection using a rhamnosidase hydrolytic mechanism. Despite the narrow host range of this phage, it is suitable for phage control application.

A Longitudinal Analysis of Methicillin-ResistantStaphylococcus aureusin a Hong Kong Teaching Hospital

2004 ◽  
Vol 25 (2) ◽  
pp. 126-129 ◽  
Author(s):  
Margaret Ip ◽  
D. J. Lyon ◽  
F. Chio ◽  
A. F. Cheng
Keyword(s):  
Hong Kong ◽  
Teaching Hospital ◽  
Dna Typing ◽  
Type A ◽  
University Teaching ◽  
Pfge Type ◽  
Type B ◽  
Isolation And Characterization ◽  
Surgical Units

AbstractObjectives:To review the incidence and trends of MRSA during a 12-year (1989-2000) period at a university teaching hospital and the relationship between strain distribution by antibiogram and molecular typing.Design:Retrospective review of laboratory-based surveillance records on MRSA isolation and characterization of strains by antimicrobial susceptibility and PFGE. A patient episode was counted at the time when MRSA was first isolated.Setting:A 1,350-bed university teaching hospital in Hong Kong.Patients:Those with clinical isolates of MRSA.Results:During 1989 to 2000, the hospital recorded 1,203,175 deaths and discharges (D&D) and encountered 5,707 patient episodes of new MRSA isolation. The overall incidence of patient episodes of MRSA was 0.47/100 D&D. In 1989, the incidence was 0.81/100 D&D and fell to a low of 0.33/100 D&D in 1995, but then rose to 0.50/100 D&D in 2000. Antibiogram and DNA typing identified 5 major types. PFGE type A constituted 68% (211/312) of isolates and was present throughout the 12-year period. PFGE type B constituted 13% (40/312) of isolates and was only present from 1995 to 2000. These isolates form a distinct clone and had unique antibiotic resistance profiles.Conclusions:The study showed the establishment of a dominant MRSA clone (PFGE type A group) in the intensive care, medical, and surgical units and the appearance of a new MRSA strain in 1995 (PFGE type B), which partly explained the rise in incidence of MRSA cases and a disproportionate rise in MRSA bacteremia from 1995 to 2000.

Development and Characterization of DNA Aptamers Against Shigella boydii and Shigella dysenteriae

2018 ◽  
Vol 12 (4) ◽  
pp. 521-527
Author(s):  
John G. Bruno ◽  
Jeffrey C. Sivils ◽  
Taylor Phillips
Keyword(s):  
Shigella Dysenteriae ◽  
Dna Aptamers ◽  
Shigella Boydii

scholarly journals Biochemical genetics of Neurospora nuclease I: Isolation and characterization of nuclease (nuc) mutants

1983 ◽  
Vol 41 (3) ◽  
pp. 271-286 ◽  
Author(s):  
A. M. Forsthoefel ◽  
N. C. Mishra
Keyword(s):  
Nucleic Acids ◽  
Type Strain ◽  
Wild Type Strain ◽  
Extracellular Enzymes ◽  
Spontaneous Mutation ◽  
Subsequent Paper ◽  
Wild Type ◽  
Isolation And Characterization ◽  
Phosphorus Source

SUMMARYIsolation and characterization of five new nuclease (nuc) deficient mutants ofNeurosporahave been described. The new mutants are unable to utilize nucleic acids as the sole phosphorus source and possess growth characteristics similar to thosenuc(nuc-1andnuc-2) mutants described previously. Two new mutants (nuc-4andnuc-5) were able to use RNA or predigested DNA (but not intact DNA) as phosphorus source and showed temperature sensitive growth at 37 °C. Based on the data from complementation and genetic analyses the five new nuc mutants (nuc-3, nuc-4, nuc-5, nuc-6andnuc-7) were found nonallelic to each other and to previously describednuc(nuc-1andnuc-2) mutants; the newnucmutants mapped to the right ofarg-12on linkage group II. On biochemical analyses, thesenucmutants were found to possess a lower level of extracellular nucleases and alkaline phosphatase as compared to the wild type strain. The ds DNase activity of the new mutants was only about 2–12% of that of the wild type strain; thus, the low level of these extracellular enzymes in thenucmutants causes their inability to utilize nucleic acids as the sole phosphorus source. Wild type levels of these enzymes were restored in the complementing heterokaryons capable of full growth on the DNA medium. Data from intercrosses, mutagen sensitivity and spontaneous mutation-frequency studies (as discussed in a subsequent paper) indicated the involvement of thenucgenes in DNA repair and recombination.

Isolation and Characterization of a Novel myovirus Infecting Shigella dysenteriae from the Aeration Tank Water

2020 ◽  
Vol 192 (1) ◽  
pp. 120-131 ◽  
Author(s):  
Han Lu ◽  
Honghui Liu ◽  
Min Lu ◽  
Jingwei Wang ◽  
Xinchun Liu ◽  
...  
Keyword(s):  
Shigella Dysenteriae ◽  
Aeration Tank ◽  
Isolation And Characterization ◽  
Tank Water

scholarly journals Characterization of cryptic flagellin genes in Shigella boydii and Shigella dysenteriae

2001 ◽  
Vol 76 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Akira Tominaga ◽  
Mohamed A.-H. Mahmoud ◽  
Abu Amar M.Al Mamun ◽  
Takafumi Mukaihara
Keyword(s):  
Shigella Dysenteriae ◽  
Shigella Boydii

scholarly journals Isolation and Characterization of a Vibrio vulnificus Mutant Deficient in Both Extracellular Metalloprotease and Cytolysin

2001 ◽  
Vol 69 (9) ◽  
pp. 5943-5948 ◽  
Author(s):  
Jong-Jin Fan ◽  
Chung-Ping Shao ◽  
Ya-Chi Ho ◽  
Chun-Keung Yu ◽  
Lien-I Hor
Keyword(s):  
Type Strain ◽  
Alimentary Tract ◽  
Wild Type Strain ◽  
Vibrio Vulnificus ◽  
Wild Type ◽  
Allelic Exchange ◽  
Isolation And Characterization

ABSTRACT We isolated a Vibrio vulnificus mutant that was deficient in both metalloprotease and cytolysin by allelic exchange. The virulence of this mutant in mice and its cytotoxicity for HEp-2 cells were comparable to those of the wild-type strain, indicating that neither factor was essential for these properties. The cytolysin, but not the protease, seemed to be important for causing damage in the alimentary tract of the mice.

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