scholarly journals Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

2020 ◽  
Vol 21 (9) ◽  
pp. 3170
Author(s):  
Mikhail M. Shneider ◽  
Anna A. Lukianova ◽  
Peter V. Evseev ◽  
Anna M. Shpirt ◽  
Marsel R. Kabilov ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Negative Impact ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Narrow Host Range ◽  
Isolation And Characterization ◽  
Control Application ◽  
Hydrolytic Mechanism

Phytopathogenic bacteria belonging to the Pectobacterium and Dickeya genera (soft-rot Pectobacteriaceae) are in the focus of agriculture-related microbiology because of their diversity, their substantial negative impact on the production of potatoes and vegetables, and the prospects of bacteriophage applications for disease control. Because of numerous amendments in the taxonomy of P. carotovorum, there are still a few studied sequenced strains among this species. The present work reports on the isolation and characterization of the phage infectious to the type strain of P. carotovorum. The phage Arno 160 is a lytic Podovirus representing a potential new genus of the subfamily Autographivirinae. It recognizes O-polysaccahride of the host strain and depolymerizes it in the process of infection using a rhamnosidase hydrolytic mechanism. Despite the narrow host range of this phage, it is suitable for phage control application.

Characterization of Pectobacterium carotovorum subsp. carotovorum Bacteriophage PP16 Prospective for Biocontrol of Potato Soft Rot

Microbiology ◽  
2019 ◽  
Vol 88 (4) ◽  
pp. 451-460 ◽  
Author(s):  
M. V. Voronina ◽  
E. N. Bugaeva ◽  
D. M. Vasiliev ◽  
A. P. Kabanova ◽  
A. P. Barannik ◽  
...  
Keyword(s):  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Carotovorum Subsp

scholarly journals Origin and Evolution of Studiervirinae Bacteriophages Infecting Pectobacterium: Horizontal Transfer Assists Adaptation to New Niches

2020 ◽  
Vol 8 (11) ◽  
pp. 1707
Author(s):  
Peter V. Evseev ◽  
Anna A. Lukianova ◽  
Mikhail M. Shneider ◽  
Aleksei A. Korzhenkov ◽  
Eugenia N. Bugaeva ◽  
...  
Keyword(s):  
New Genus ◽  
Soft Rot ◽  
Broad Host Range ◽  
Gene Exchange ◽  
Genomic Features ◽  
Origin And Evolution ◽  
Phage T7 ◽  
Tail Spike

Black leg and soft rot are devastating diseases causing up to 50% loss of potential potato yield. The search for, and characterization of, bacterial viruses (bacteriophages) suitable for the control of these diseases is currently a sought-after task for agricultural microbiology. Isolated lytic Pectobacterium bacteriophages Q19, PP47 and PP81 possess a similar broad host range but differ in their genomic properties. The genomic features of characterized phages have been described and compared to other Studiervirinae bacteriophages. Thorough phylogenetic analysis has clarified the taxonomy of the phages and their positioning relative to other genera of the Autographiviridae family. Pectobacterium phage Q19 seems to represent a new genus not described previously. The genomes of the phages are generally similar to the genome of phage T7 of the Teseptimavirus genus but possess a number of specific features. Examination of the structure of the genes and proteins of the phages, including the tail spike protein, underlines the important role of horizontal gene exchange in the evolution of these phages, assisting their adaptation to Pectobacterium hosts. The results provide the basis for the development of bacteriophage-based biocontrol of potato soft rot as an alternative to the use of antibiotics.

scholarly journals Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent Klebsiella pneumoniae Strain 52145, and Identification of Its Functional Depolymerase

2021 ◽  
Vol 9 (3) ◽  
pp. 650
Author(s):  
Botond Zsombor Pertics ◽  
Alysia Cox ◽  
Adrienn Nyúl ◽  
Nóra Szamek ◽  
Tamás Kovács ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Antimicrobial Agents ◽  
Lytic Bacteriophage ◽  
Bacterial Cells ◽  
Narrow Host Range ◽  
Isolation And Characterization ◽  
Different Temperatures ◽  
Colonization Model

Klebsiella pneumoniae is among the leading bacteria that cause nosocomial infections. The capsule of this Gram-negative bacterium is a dominant virulence factor, with a prominent role in defense and biofilm formation. Bacteriophages, which are specific for one bacterial strain and its capsule type, can evoke the lysis of bacterial cells, aided by polysaccharide depolymerase enzymes. In this study, we isolated and characterized a bacteriophage against the nosocomial K. pneumoniae 52145 strain with K2 capsular serotype. The phage showed a narrow host range and stable lytic activity, even when exposed to different temperatures or detergents. Preventive effect of the phage in a nasal colonization model was investigated in vivo. Phlyogenetic analysis showed that the newly isolated Klebsiella phage B1 belongs to the Webervirus genus in Drexlerviridae family. We identified the location of the capsule depolymerase gene of the new phage, which was amplified, cloned, expressed, and purified. The efficacy of the recombinant B1dep depolymerase was tested by spotting on K. pneumoniae strains and it was confirmed that the extract lowers the thickness of the bacterium lawn as it degrades the protective capsule on bacterial cells. As K. pneumoniae strains possessing the K2 serotype have epidemiological importance, the B1 phage and its depolymerase are promising candidates for use as possible antimicrobial agents.

Characterization of Pectobacterium carotovorum subsp. carotovorum causing soft-rot disease on Pinellia ternata in China

2007 ◽  
Vol 120 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Xiu-Fang Hu ◽  
Fei-Xiang Ying ◽  
Yu-Bo He ◽  
Yuan-Yuan Gao ◽  
Hai-Min Chen ◽  
...  
Keyword(s):  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Pinellia Ternata ◽  
Soft Rot Disease ◽  
Carotovorum Subsp ◽  
Rot Disease

scholarly journals Isolation and Characterization of Pectobacterium Phage vB_PatM_CB7: New Insights into the Genus Certrevirus

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 352
Author(s):  
Colin Buttimer ◽  
Caoimhe Lynch ◽  
Hanne Hendrix ◽  
Horst Neve ◽  
Jean-Paul Noben ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Burst Size ◽  
Homing Endonuclease ◽  
Soft Rot ◽  
Nucleotide Metabolism ◽  
Homing Endonucleases ◽  
Isolation And Characterization ◽  
Rot Disease ◽  
Large Terminase

To date, Certrevirus is one of two genera of bacteriophage (phage), with phages infecting Pectobacterium atrosepticum, an economically important phytopathogen that causes potato blackleg and soft rot disease. This study provides a detailed description of Pectobacterium phage CB7 (vB_PatM_CB7), which specifically infects P. atrosepticum. Host range, morphology, latent period, burst size and stability at different conditions of temperature and pH were examined. Analysis of its genome (142.8 kbp) shows that the phage forms a new species of Certrevirus, sharing sequence similarity with other members, highlighting conservation within the genus. Conserved elements include a putative early promoter like that of the Escherichia coli sigma70 promoter, which was found to be shared with other genus members. A number of dissimilarities were observed, relating to DNA methylation and nucleotide metabolism. Some members do not have homologues of a cytosine methylase and anaerobic nucleotide reductase subunits NrdD and NrdG, respectively. Furthermore, the genome of CB7 contains one of the largest numbers of homing endonucleases described in a single phage genome in the literature to date, with a total of 23 belonging to the HNH and LAGLIDADG families. Analysis by RT-PCR of the HNH homing endonuclease residing within introns of genes for the large terminase, DNA polymerase, ribonucleotide reductase subunits NrdA and NrdB show that they are splicing competent. Electrospray ionization-tandem mass spectrometry (ESI-MS/MS) was also performed on the virion of CB7, allowing the identification of 26 structural proteins—20 of which were found to be shared with the type phages of the genera of Vequintavirus and Seunavirus. The results of this study provide greater insights into the phages of the Certrevirus genus as well as the subfamily Vequintavirinae.

Isolation and characterization of cellulolytic anaerobic fungi and associated mycoplasmas from the rumen of a steer fed a roughage diet

1990 ◽  
Vol 36 (7) ◽  
pp. 513-517 ◽  
Author(s):  
H. Kudo ◽  
K. D. Jakober ◽  
R. C. Phillippe ◽  
K.-J. Cheng ◽  
D. J. S. Barr ◽  
...  
Keyword(s):  
Key Words ◽  
New Genus ◽  
Anaerobic Fungi ◽  
Ecological Role ◽  
New Genus And Species ◽  
Fermentation Products ◽  
Isolation And Characterization ◽  
Neocallimastix Patriciarum

The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation patterns were very similar to those of rumen fungi isolated in other countries. Mycoplasmas were found to be associated with 7 of the 12 isolates. The ecological role of the association of the mycoplasmas with rumen fungi is still unknown. Key words: Orpinomyces bovis, Piromyces communis, Neocallimastix patriciarum.

scholarly journals Characterization of onion soft rot bacteria in Bangladesh

2017 ◽  
Vol 52 (3) ◽  
pp. 209-220
Author(s):  
MM Rahman ◽  
MAA Khan ◽  
IH Mian ◽  
AM Akanda ◽  
MZ Alam
Keyword(s):  
Bacterial Pathogens ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Carotovorum Subsp ◽  
O 15 ◽  
O 69 ◽  
Physiological And Biochemical

A study was undertaken for characterization and identification of the soft rot causing bacterial pathogens of onion. Bacterial pathogens were isolated from soft rotted stored onions of different varieties and locations of Bangladesh. Altogether 73 bacterial isolates were isolated from soft rotted onions. Among them, twelve soft rot-positive isolates were selected for characterization and identification on the basis of more virulence. Physiological and biochemical tests were performed following standard methods for characterization and identification of selected soft rot bacterial isolates. Seven isolates namely O-03, O-18, O-69, O-72, O-130, O-156 and O-180 were identified as Pectobacterium carotovorum subsp. carotovorum (E. carotovora subsp. carotovora), two isolates O-101 and O-118 were identified as E. chrysanthemi and three isolates O-05, O-14 and O-15 were the members of Burkholderia cepacia.Bangladesh J. Sci. Ind. Res. 52(3), 209-220, 2017

Isolation and characterization of provisional serovar Shigella boydii E16553 from diarrhoeal patients in Bangladesh

2005 ◽  
Vol 54 (5) ◽  
pp. 477-480 ◽  
Author(s):  
M Ansaruzzaman ◽  
Marzia Sultana ◽  
Kaisar A Talukder ◽  
K Alam ◽  
S Matsushita ◽  
...  
Keyword(s):  
Type Strain ◽  
Shigella Dysenteriae ◽  
Pfge Type ◽  
Common Source ◽  
Reference Laboratory ◽  
Japanese Strain ◽  
Shigella Boydii ◽  
Virulence Markers ◽  
Isolation And Characterization

In previous studies with strains of the Shigella dysenteriae provisional serovars E22383 and E23507 from diarrhoeal stools from patients in Bangladesh, two strains of Shigella species were identified as Shigella boydii provisional serovar E16553 by a reference laboratory. Further tests with an antiserum to an international type strain of the provisional serovar E16553 identified an additional 15 isolates. None of the isolates reacted with antisera to the established Shigella serovars or any other provisional serovars reported so far and all showed biochemical reactions typical of S. boydii. All of the isolates harboured the 140 MDa invasion plasmid, had the ipaH gene and produced keratoconjunctivitis in the guinea pig eye. All isolates were susceptible to ampicillin, sulfamethoxazole-trimethoprim, nalidixic acid, ciprofloxacin and mecillinam but eight strains were resistant to tetracycline. A single PFGE type (type A) was shown for all 17 clinical isolates, indicating a common source of origin. The pulsotype of the Bangladeshi isolates was closely related to that of a Japanese strain but was different from that of the type strain. On the basis of these biochemical, serological and virulence markers, and diverse geographical origin, it is recommended that the provisional status of serovar E16553 be changed and that it be included in the international serotyping classification scheme as S. boydii 19.

scholarly journals Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia

2011 ◽  
Vol 77 (5) ◽  
pp. 292-294 ◽  
Author(s):  
Eisa Nazerian ◽  
Kamaruzaman Sijam ◽  
Zainal Abidin Meor Ahmad ◽  
Kavous Keshavarz
Keyword(s):  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Soft Rot Disease ◽  
Rot Disease

scholarly journals Isolation and complete genome sequencing of Pectobacterium phage Jarilo, representing a novel genus of bacteriophages within the subfamily Autographivirinae

2020 ◽  
Author(s):  
Julie Stenberg Pedersen ◽  
Alexander Byth Carstens ◽  
Amaru Miranda Djurhuus ◽  
Witold Kot ◽  
Lars Hestbjerg Hansen
Keyword(s):  
Soft Rot ◽  
Open Reading Frames ◽  
Novel Genus ◽  
The Past ◽  
Isolation And Characterization ◽  
Double Stranded Dna ◽  
Plant Disease Control ◽  
Phage T7 ◽  
Reading Frames

AbstractPectobacterium carotovorum is the causative agent of bacterial soft rot on various plant species. The use of phages for plant disease control have gained increased awareness over the past years. We here describe the isolation and characterization of Pectobacterium phage Jarilo, representing a novel genus of bacteriophages within the subfamily Autographivirinae. Jarilo possesses a double-stranded DNA genome of 40557 bp with a G+C% content of 50.08% and 50 predicted open reading frames (ORFs). Gene synteny and products seem to be somewhat conserved between Pectobacterium phage Jarilo and Enterobacteria phage T7, but limited nucleotide similarity is found between Jarilo and other phages within the subfamily Autographivirinae. We propose Pectobacterium phage Jarilo as the first member of a new genus of bacteriophages within the subfamily Autographivirinae.

Sign in / Sign up

Export Citation Format

Share Document