Implication of the C terminus of the Prunus necrotic ringspot virus movement protein in cell-to-cell transport and in its interaction with the coat protein

We previously showed that the movement protein (MP) gene of Alfalfa mosaic virus (AMV) is functionally exchangeable for the cell-to-cell transport of the corresponding genes of Tobacco mosaic virus (TMV), Brome mosaic virus, Prunus necrotic ringspot virus, Cucumber mosaic virus and Cowpea mosaic virus. We have analysed the capacity of the heterologous MPs to systemically transport the corresponding chimeric AMV genome. All MPs were competent in systemic transport but required the fusion at their C terminus of the coat protein-interacting C-terminal 44 aa (A44) of the AMV MP. Except for the TMV MP, the presence of the hybrid virus in upper leaves correlated with the capacity to move locally. These results suggest that all the MPs assigned to the 30K superfamily should be exchangeable not only for local virus movement but also for systemic transport when the A44 fragment is present.

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Faculty Opinions recommendation of MPB2C, a microtubule-associated plant protein binds to and interferes with cell-to-cell transport of tobacco mosaic virus movement protein.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1015658.197703 ◽

2003 ◽

Author(s):

Christopher Staiger

Keyword(s):

Tobacco Mosaic Virus ◽

Mosaic Virus ◽

Movement Protein ◽

Plant Protein ◽

Virus Movement ◽

Cell Transport

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Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions

Virology Journal ◽

10.1186/1743-422x-5-61 ◽

2008 ◽

Vol 5 (1) ◽

pp. 61 ◽

Cited By ~ 8

Author(s):

Eric van der Walt ◽

Kenneth E Palmer ◽

Darren P Martin ◽

Edward P Rybicki

Keyword(s):

Coat Protein ◽

Protein Interactions ◽

Movement Protein ◽

Maize Streak Virus ◽

Virus Movement ◽

Streak Virus ◽

Biological Importance

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Compatibility of the movement protein and the coat protein of cucumoviruses is required for cell-to-cell movement

Journal of General Virology ◽

10.1099/vir.0.19687-0 ◽

2004 ◽

Vol 85 (4) ◽

pp. 1039-1048 ◽

Cited By ~ 18

Author(s):

Katalin Salánki ◽

Ákos Gellért ◽

Emese Huppert ◽

Gábor Náray-Szabó ◽

Ervin Balázs

Keyword(s):

Coat Protein ◽

Movement Protein ◽

Point Mutations ◽

Cell Movement ◽

Virus Movement ◽

Tomato Aspermy Virus ◽

Sequence Encoding ◽

Surface Electrostatic Potential ◽

Test Plants ◽

Insight Into

For the cell-to-cell movement of cucumoviruses both the movement protein (MP) and the coat protein (CP) are required. These are not reversibly exchangeable between Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV). The MP of CMV is able to function with the TAV CP (chimera RT), but TAV MP is unable to promote the cell-to-cell movement in the presence of CMV CP (chimera TR). To gain further insight into the non-infectious nature of the TR recombinant, RNA 3 chimeras were constructed with recombinant MPs and CPs. The chimeric MP and one of the CP recombinants were infectious. The other recombinant CP enabled virus movement only after the introduction of two point mutations (Glu→Lys and Lys→Arg at aa 62 and 65, respectively). The mutations served to correct the CP surface electrostatic potential that was altered by the recombination. The infectivity of the TR virus on different test plants was restored by replacing the sequence encoding the C-terminal 29 aa of the MP with the corresponding sequence of the CMV MP gene or by exchanging the sequence encoding the C-terminal 15 aa of the CP with the same region of TAV. The analysis of the recombinant clones suggests a requirement for compatibility between the C-terminal 29 aa of the MP and the C-terminal two-thirds of the CP for cell-to-cell movement of cucumoviruses.

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