scholarly journals Sulfated galactans isolated from the red seaweed Gracilaria fisheri target the envelope proteins of white spot syndrome virus and protect against viral infection in shrimp haemocytes

2014 ◽  
Vol 95 (5) ◽  
pp. 1126-1134 ◽  
Author(s):  
Tawut Rudtanatip ◽  
Somluk Asuvapongpatana ◽  
Boonsirm Withyachumnarnkul ◽  
Kanokpan Wongprasert
Keyword(s):  
Viral Load ◽  
Viral Infection ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Red Seaweed ◽  
Viral Attachment ◽  
Sulfated Galactans ◽  
Cell Mortality ◽  
White Spot Syndrome

The present study was aimed at evaluating an underlying mechanism of the antiviral activity of the sulfated galactans (SG) isolated from the red seaweed Gracilaria fisheri against white spot syndrome virus (WSSV) infection in haemocytes of the black tiger shrimp Penaeus monodon. Primary culture of haemocytes from Penaeus monodon was performed and inoculated with WSSV, after which the cytopathic effect (CPE), cell viability and viral load were determined. Haemocytes treated with WSSV-SG pre-mix showed decreased CPE, viral load and cell mortality from the viral infection. Solid-phase virus-binding assays revealed that SG bound to WSSV in a dose-related manner. Far Western blotting analysis indicated that SG bound to VP 26 and VP 28 proteins of WSSV. In contrast to the native SG, desulfated SG did not reduce CPE and cell mortality, and showed low binding activity with WSSV. The current study suggests that SG from Gracilaria fisheri elicits its anti-WSSV activity by binding to viral proteins that are important for the process of viral attachment to the host cells. It is anticipated that the sulfate groups of SG are important for viral binding.

scholarly journals Co-Infection of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV) in the Wild Crustaceans of Andaman and Nicobar Archipelago, India

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1378
Author(s):  
Kandasamy Saravanan ◽  
Jayasimhan Praveenraj ◽  
Rajendran Kiruba-Sankar ◽  
Varsha Devi ◽  
Utpal Biswas ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Scylla Serrata ◽  
Histopathological Analysis ◽  
Necrosis Virus ◽  
High Sequence Identity ◽  
In The Wild ◽  
White Spot Syndrome

The present study was intended to screen the wild crustaceans for co-infection with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV) in Andaman and Nicobar Archipelago, India. We screened a total of 607 shrimp and 110 crab samples using a specific polymerase chain reaction, and out of them, 82 shrimps (13.5%) and 5 (4.5%) crabs were found positive for co-infection of IHHNV and WSSV. A higher rate of co-infection was observed in Penaeus monodon and Scylla serrata than other shrimp and crab species. The nucleotide sequences of IHHNV and WSSV obtained from crab in this present study exhibited very high sequence identity with their counterparts retrieved from various countries. Histopathological analysis of the infected shrimp gill sections further confirmed the eosinophilic intra-nuclear cowdry type A inclusion bodies and basophilic intra-nuclear inclusion bodies characteristics of IHHNV and WSSV infections, respectively. The present study serves as the first report on co-infection of WSSV and IHHNV in Andaman and Nicobar Archipelago, India and accentuates the critical need for continuous monitoring of wild crustaceans and appropriate biosecurity measures for brackishwater aquaculture.

scholarly journals DETEKSI WHITE SPOT SYNDROME VIRUS(WSSV) PADA UDANG WINDU (Penaeus monodon) DI BALI DAN JAWA TIMUR MENGGUNAKAN METODE POLYMERASE CHAIN REACTIOIN(PCR)

2017 ◽  
Vol 10 (1) ◽  
pp. 55
Author(s):  
Ketut Mahardika ◽  
Zafran Zafran ◽  
Lsti Koesharyani
Keyword(s):  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Polymerase Chain ◽  
White Spot Syndrome

White spot syndrome (WSS) telah ditemukan pada banyak spesies udang dan kepiting, tidak saja di Asia tetapi hampir di seluruh dunia.

scholarly journals Identification of a Shrimp E3 Ubiquitin Ligase TRIM50-Like Involved in Restricting White Spot Syndrome Virus Proliferation by Its Mediated Autophagy and Ubiquitination

2021 ◽  
Vol 12 ◽  
Author(s):  
Chao Zhao ◽  
Chao Peng ◽  
Pengfei Wang ◽  
Lulu Yan ◽  
Sigang Fan ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Positive Role ◽  
Protein Levels ◽  
Mrna And Protein Expression ◽  
White Spot Syndrome ◽  
Critical Components ◽  
Trim Proteins

Most tripartite motif (TRIM) family proteins are critical components of the autophagy machinery and play important roles in host defense against viral pathogens in mammals. However, the roles of TRIM proteins in autophagy and viral infection have not been studied in lower invertebrates, especially crustaceans. In this study, we first identified a TRIM50-like gene from Penaeus monodon (designated PmTRIM50-like), which, after a white spot syndrome virus (WSSV) challenge, was significantly upregulated at the mRNA and protein levels in the intestine and hemocytes. Knockdown of PmTRIM50-like led to an increase in the WSSV quantity in shrimp, while its overexpression led to a decrease compared with the controls. Autophagy can be induced by WSSV or rapamycin challenge and has been shown to play a positive role in restricting WSSV replication in P. monodon. The mRNA and protein expression levels of PmTRIM50-like significantly increased with the enhancement of rapamycin-induced autophagy. The autophagy activity induced by WSSV or rapamycin challenge could be inhibited by silencing PmTRIM50-like in shrimp. Further studies showed that rapamycin failed to induce autophagy or inhibit WSSV replication after knockdown of PmTRIM50-like. Moreover, pull-down and in vitro ubiquitination assays demonstrated that PmTRIM50-like could interact with WSSV envelope proteins and target them for ubiquitination in vitro. Collectively, this study demonstrated that PmTRIM50-like is required for autophagy and is involved in restricting the proliferation of WSSV through its ubiquitination. This is the first study to report the role of a TRIM family protein in virus infection and host autophagy in crustaceans.

scholarly journals Evaluation of an immunodot test to manage white spot syndrome virus (WSSV) during cultivation of the giant tiger shrimp Penaeus monodon

2008 ◽  
Vol 79 ◽  
pp. 157-161 ◽  
Author(s):  
R Patil ◽  
KJ Palaksha ◽  
TM Anil ◽  
Guruchannabasavanna ◽  
P Patil ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Tiger Shrimp ◽  
White Spot Syndrome

scholarly journals Seasonal status of white spot syndrome virus in broodstocks, nauplii and postlarvae of black tiger shrimp (Penaeus monodon) in Bangladesh

1970 ◽  
Vol 1 (3) ◽  
pp. 56-61 ◽  
Author(s):  
MM Iqbal ◽  
MA Kabir ◽  
CB Alan ◽  
MAA Mamun ◽  
MM Hossain
Keyword(s):  
White Spot Syndrome Virus ◽  
Monsoon Season ◽  
Penaeus Monodon ◽  
Winter Season ◽  
White Spot ◽  
Water Current ◽  
Fluctuation Pattern ◽  
Three Stages ◽  
Polymerase Chain ◽  
White Spot Syndrome

Seasonal variation of white spot syndrome virus (WSSV) prevalence in broodstocks shrimp, nauplii and postlarvae were investigated from 2004 to 2006 in Bangladesh by polymerase chain reaction (PCR) assay. There was consistent pattern of fluctuation of WSSV prevalence in broodstocks, nauplii and postlarvae. WSSV fluctuation pattern in three stages were more or less similar for each year. Average lowest prevalence in broodstock was 0% in September and highest was 90% in May-June during the monsoon season in Bangladesh. The WSSV fluctuation patterns for nauplius correlated with broodstock patterns with average lowest prevalence of 0% in September compared to the highest of 40% in July. Similarly, average lowest prevalence of WSSV in postlarvae was 0% in December and highest was 14% in July. It was evident from this study that WSSV prevalence in broodstock, nauplius and postlarvae remained high during the monsoon season and low during the winter season when the water current, salinity and tidal flashing remained more or less stable in the Bay of Bengal in Bangladesh. Key words: Bangladesh; broodstocks; nauplii; postlarvae; seasonal; shrimp; WSSV. DOI: http://dx.doi.org/10.3329/ijns.v1i3.8822 International Journal of Natural Sciences (2011), 1(3):56-61

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