Analysis of the viability of coat-protein hybrids between Cucumber mosaic virus and Tomato aspermy virus

Coat-protein (CP) hybrids between Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV) were engineered to analyse reported CP-associated differences between these viruses. CP portions delimited by aa 1–59, 60–148 and 149–219 were exchanged in all possible combinations within TAV RNA3. The seven possible chimeras were able to replicate in tobacco protoplasts to similar levels, but only those having residues 1–59 or 60–148 from CMV were infectious to tobacco plants, a common host for CMV and TAV, and formed stable particles. When most of the movement protein (MP) of TAV was substituted for that of CMV, infectivity of CP hybrids did not vary. No hybrid was able to infect cucumber plants, a host for CMV and not for TAV. Need for MP–CP compatibility could explain these results, but shows that MP–CP compatibility conditions the use of CP chimeras to map CP-associated differences between CMV and TAV.

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Expression of the gene encoding the coat protein of cucumber mosaic virus (CMV) strain WL appears to provide protection to tobacco plants against infection by several different CMV strains

Gene ◽

10.1016/0378-1119(91)90317-5 ◽

1991 ◽

Vol 107 (2) ◽

pp. 181-188 ◽

Cited By ~ 32

Author(s):

Namba Shigetou ◽

Ling Kaishu ◽

Carol Gonsalves ◽

Dennis Gonsalves ◽

Jerry L. Slightom

Keyword(s):

Coat Protein ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Gene Encoding ◽

Tobacco Plants

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Protection against cucumber mosaic virus (CMV) strains O and Y and chrysanthemum mild mottle virus in transgenic tobacco plants expressing CMV-O coat protein

Journal of General Virology ◽

10.1099/0022-1317-74-2-319 ◽

1993 ◽

Vol 74 (2) ◽

pp. 319-322 ◽

Cited By ~ 24

Author(s):

M. Nakajima ◽

T. Hayakawa ◽

I. Nakamura ◽

M. Suzuki

Keyword(s):

Coat Protein ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Transgenic Tobacco ◽

Mottle Virus ◽

Transgenic Tobacco Plants ◽

Tobacco Plants

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Comparative Susceptibility of Transgenic Tobacco Plants and Protoplasts Expressing the Coat Protein Gene of Cucumber Mosaic Virus to Infection with Virions and RNA

Phytopathology ◽

10.1094/phyto-83-542 ◽

1993 ◽

Vol 83 (5) ◽

pp. 542 ◽

Cited By ~ 15

Author(s):

Tetsuro Okuno

Keyword(s):

Coat Protein ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Transgenic Tobacco ◽

Coat Protein Gene ◽

Protein Gene ◽

Transgenic Tobacco Plants ◽

Tobacco Plants ◽

Comparative Susceptibility

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Viral Protection in Transgenic Tobacco Plants Expressing the Cucumber Mosaic Virus Coat Protein or its Antisense RNA

Nature Biotechnology ◽

10.1038/nbt0588-549 ◽

1988 ◽

Vol 6 (5) ◽

pp. 549-557 ◽

Cited By ~ 113

Author(s):

Maria Cuozzo ◽

Keith M. O'Connell ◽

Wojciech Kaniewski ◽

Rong-Xiang Fang ◽

Nam-Hai Chua ◽

...

Keyword(s):

Coat Protein ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Transgenic Tobacco ◽

Antisense Rna ◽

Transgenic Tobacco Plants ◽

Tobacco Plants ◽

Virus Coat Protein ◽

Virus Coat

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Conversion in the Requirement of Coat Protein in Cell-to-Cell Movement Mediated by the Cucumber Mosaic Virus Movement Protein

Journal of Virology ◽

10.1128/jvi.75.17.8045-8053.2001 ◽

2001 ◽

Vol 75 (17) ◽

pp. 8045-8053 ◽

Cited By ~ 47

Author(s):

Hideaki Nagano ◽

Kazuyuki Mise ◽

Iwao Furusawa ◽

Tetsuro Okuno

Keyword(s):

Amino Acids ◽

Coat Protein ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Movement Protein ◽

Cell Movement ◽

Plant Viruses ◽

Brome Mosaic Virus ◽

Viral Movement ◽

Intercellular Movement

ABSTRACT Plant viruses have movement protein (MP) gene(s) essential for cell-to-cell movement in hosts. Cucumber mosaic virus (CMV) requires its own coat protein (CP) in addition to the MP for intercellular movement. Our present results using variants of both CMV and a chimeric Brome mosaic virus with the CMV MP gene revealed that CMV MP truncated in its C-terminal 33 amino acids has the ability to mediate viral movement independently of CP. Coexpression of the intact and truncated CMV MPs extremely reduced movement of the chimeric viruses, suggesting that these heterogeneous CMV MPs function antagonistically. Sequential deletion analyses of the CMV MP revealed that the dispensability of CP occurred when the C-terminal deletion ranged between 31 and 36 amino acids and that shorter deletion impaired the ability of the MP to promote viral movement. This is the first report that a region of MP determines the requirement of CP in cell-to-cell movement of a plant virus.

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Effectiveness of coat protein and defective replicase gene-mediated resistance against Australian isolates of cucumber mosaic virus

Australian Journal of Experimental Agriculture ◽

10.1071/ea96097 ◽

1998 ◽

Vol 38 (4) ◽

pp. 375 ◽

Cited By ~ 6

Author(s):

Z. Singh ◽

M. G. K. Jones ◽

R. A. C. Jones

Keyword(s):

Coat Protein ◽

Transgenic Plants ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Transgenic Tobacco ◽

Extreme Resistance ◽

Tobacco Plants ◽

Systemic Movement ◽

Cp Gene ◽

Subgroup Ii

Summary. Transgenic tobacco (Nicotiana tabacum) plants of (i) cv. Samsun NN containing the cauliflower mosaic virus 35S constitutive promoter linked to a defective replicase (DR) gene derived from cucumber mosaic virus (CMV) subgroup I isolate Fny, and (ii) cv. Xanthi containing the CaMV 35S promoter linked to the coat protein (CP) gene of CMV subgroup I isolate C were tested for resistance to various Australian isolates of CMV. The tobacco plants were challenged with 3 CMV subgroup 1 isolates (BNRR, BMR and B6) using sap inoculation. When used to challenge non-transgenic tobacco plants with 5 subgroup II CMV isolates from lupins (LY, LCH, LAcc, LGu and LD), this inoculation method did not result in systemic infection so graft inoculation was used instead to challenge transgenic plants with these 5 isolates. When plants of the line with the DR gene were challenged with the 3 subgroup I isolates, extreme resistance was revealed as none showed symptoms and CMV was not detectable by ELISA. When the same 3 isolates were inoculated to the 3 lines with the CP gene, resistance was characterised by fewer plants becoming virus infected, delayed systemic movement and, in the plants that were infected, partial remission of symptoms plus somewhat decreased virus concentration. Challenge of transgenic plants with DR or CP with the 5 subgroup II isolates resulted in fewer plants becoming infected. Actual numbers of plants infected varied with line and subgroup II isolate and the DR gene was as effective as the CP gene at decreasing infection. With subgroup II isolate LY, infection was associated with remission of symptoms and with the other 4 isolates with delayed systemic movement. Thus the DR gene approach was more effective than the CP approach in obtaining extreme resistance against Australian subgroup I isolates of CMV. These results suggest that introducing a similar DR gene construct made from a subgroup II isolate from lupins into commercial lupin cultivars may be a suitable strategy for obtaining extreme resistance to subgroup II isolates from lupins.

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