Highly-efficient Cpf1-mediated gene targeting in mice following high concentration pronuclear injection
ABSTRACTCpf1 has emerged as an alternative to the Cas9 RNA-guided nuclease. Here we show that gene targeting rates in mice using Cpf1 can meet or even surpass Cas9 targeting rates (approaching 100% targeting) but require higher concentrations of mRNA and guide. We also demonstrate that co-injecting two guides with close targeting sites can result in synergistic genomic cutting, even if one of the guides has minimal cutting activity.
2016 ◽
Vol 4
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pp. 12014-12019
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1992 ◽
Vol 89
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pp. 5128-5132
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2018 ◽
Vol 334
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pp. 995-1005
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2008 ◽
Vol 46
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pp. 587-592
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2007 ◽
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pp. 770-775
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2017 ◽
Vol 63
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pp. 172-178
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