scholarly journals Major oscillations in spontaneous home-cage activity with an infraradian periodicity in C57Bl/6 mice housed under constant conditions

2020 ◽  
Author(s):  
K. Pernold ◽  
E. Rullman ◽  
B. Ulfhake
Keyword(s):  
Spontaneous Activity ◽  
Feeding Behaviour ◽  
Home Cage ◽  
Light Cycle ◽  
Dark Light ◽  
Cage Activity ◽  
Free Running ◽  
The Mean ◽  
Home Cage Activity

AbstractUsing 14-20 months of cumulative 24/7 home-cage activity recorded with a non-intrusive technique and a data driven analytical approach, we here provide evidence for the existence of a circannual oscillation (1-2 SD of the mean, on average 65% higher during peak of highs than lows; P=7E-50) in spontaneous activity of male and female C57BL/6 mice held under constant barrier conditions (dark-light cycle 12/12 h (DL), temperature 21±1°C, humidity 40-60%). The periodicity of the season-like oscillation is in the range of 2-4 months (on average 97 days across cohorts of cages) and off-sets also responses to environmental stimuli but does not significantly alter the preference for activity during the dark hours of this nocturnal mouse strain (P=0.11 difference between highs and lows).The significance of this hitherto not recognized slow rhythmic alteration in spontaneous activity is further substantiated by its co-variation with the feeding behaviour of the mice. The absence of coordination within and between cohorts of cages or synchronization to the seasons of the year, suggests that the oscillation of in-cage activity and behavioural responses is generated by a free-running intrinsic oscillator devoid of synchronization with an out-of-cage environmental time-keeper. Since the variation over time has such a magnitude and correlate with the feeding behaviour it is likely that it will impact a range of long term experiments conducted on laboratory mice if left unrecognized.

scholarly journals Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karin Pernold ◽  
Eric Rullman ◽  
Brun Ulfhake
Keyword(s):  
Life Science ◽  
Home Cage ◽  
Science Research ◽  
Male And Female ◽  
Genetic Manipulations ◽  
Cage Activity ◽  
Normal Behavior ◽  
Free Running ◽  
The Mean ◽  
Home Cage Activity

AbstractThe mouse is the most important mammalian model in life science research and the behavior of the mouse is a key read-out of experimental interventions and genetic manipulations. To serve this purpose a solid understanding of the mouse normal behavior is a prerequisite. Using 14–19 months of cumulative 24/7 home-cage activity recorded with a non-intrusive technique, evidence is here provided for a highly significant circannual oscillation in spontaneous activity (1–2 SD of the mean, on average 65% higher during peak of highs than lows; P = 7E−50) of male and female C57BL/6 mice held under constant conditions. The periodicity of this hitherto not recognized oscillation is in the range of 2–4 months (average estimate was 97 days across cohorts of cages). It off-sets responses to environmental stimuli and co-varies with the feeding behavior but does not significantly alter the preference for being active during the dark hours. The absence of coordination of this rhythmicity between cages with mice or seasons of the year suggest that the oscillation of physical activity is generated by a free-running intrinsic oscillator devoid of external timer. Due to the magnitude of this rhythmic variation it may be a serious confounder in experiments on mice if left unrecognized.

scholarly journals Tolerance and dependence to Δ9-tetrahydrocannabinol in rhesus monkeys: activity assessments

2018 ◽  
Author(s):  
David R. Schulze ◽  
Lance McMahon
Keyword(s):  
Rhesus Monkeys ◽  
Home Cage ◽  
Physical Dependence ◽  
Activity Maximum ◽  
Cage Activity ◽  
Cannabis Withdrawal ◽  
Abrupt Discontinuation ◽  
Home Cage Activity ◽  
Related Increase

Cannabis withdrawal upon discontinuation of long-term, heavy Cannabis use is reported in humans; however, methods to establish the nature and intensity of cannabinoid withdrawal, especially directly observable signs, have not been widely established. This study quantified activity in the home cage of rhesus monkeys, and examined the extent to which activity can be used to quantify tolerance to and dependence on Δ9-tetrahydrocannabinol (Δ9-THC). Home-cage activity was measured in one group that received Δ9-THC (1 mg/kg s.c.) every 12 h (i.e., chronic Δ9-THC), and a second group that received Δ9-THC (0.1 mg/kg i.v.) once every 3 days (i.e., intermittent Δ9-THC). Treatment was temporarily discontinued in the chronic Δ9-THC group and the effects of rimonabant and Δ9-THC were examined in both groups. Activity counts were highest during the day (lights on 0600-2000 h) and were lower at night. Rimonabant (0.1-3.2 mg/kg i.v.) dose-dependently increased activity (maximum 20-fold) in the chronic Δ9-THC group, but did not significantly alter activity in the intermittent Δ9-THC group. Δ9-THC (0.32-3.2 mg/kg i.v.) dose-dependently decreased activity counts (maximum 4-fold) in both groups, but was somewhat more potent in the intermittent as compared with the Δ9-THC group. Discontinuation of Δ9-THC treatment resulted in an immediate (i.e., within 24 h) and time-related increase in activity. Resumption of Δ9-THC treatment (1 mg/kg/12 h) produced hypoactivity that was no longer evident within 9 days of treatment. The time-related increase in home-cage activity upon abrupt discontinuation of chronic Δ9-THC treatment, as well as the effects of rimonabant to increase activity in monkeys receiving chronic, but not intermittent, Δ9-THC treatment, are consistent with signs of physical dependence on Δ9-THC in primates.

scholarly journals Long-term home cage activity scans reveal lowered exploratory behaviour in symptomatic female Rett mice

2013 ◽  
Vol 250 ◽  
pp. 148-156 ◽  
Author(s):  
Lianne Robinson ◽  
Andrea Plano ◽  
Stuart Cobb ◽  
Gernot Riedel
Keyword(s):  
Home Cage ◽  
Exploratory Behaviour ◽  
Cage Activity ◽  
Home Cage Activity

scholarly journals Impulsivity and home-cage activity are decreased by lentivirus-mediated silencing of serotonin transporter in the rat hippocampus

2013 ◽  
Vol 548 ◽  
pp. 38-43 ◽  
Author(s):  
Francesca Zoratto ◽  
Amanda L. Tringle ◽  
Giancarlo Bellenchi ◽  
Luisa Speranza ◽  
Domenica Travaglini ◽  
...  
Keyword(s):  
Serotonin Transporter ◽  
Home Cage ◽  
Rat Hippocampus ◽  
Cage Activity ◽  
Home Cage Activity

scholarly journals Influence of mouse strain, infective dose and larval burden in the brain on activity in Toxocara-infected mice

2001 ◽  
Vol 75 (1) ◽  
pp. 23-32 ◽  
Author(s):  
D.M. Cox ◽  
C.V. Holland
Keyword(s):  
Mouse Strain ◽  
Post Infection ◽  
Home Cage ◽  
Brain Activity ◽  
Toxocara Canis ◽  
Cage Activity ◽  
Home Cage Activity ◽  
The Brain ◽  
Larval Recovery ◽  
Larval Burden

Outbred LACA mice and inbred NIH mice were administered low (100 ova), medium (1000 ova), high (3000 ova) and trickle (4×250 ova) doses ofToxocara canisova and the effect of infection on activity was examined with respect to: (i) the dose of ova administered and (ii) the number of larvae recovered from the brain. Larval recovery from the brain was significantly reduced in NIH mice compared to LACA mice for the 1000, 3000 and trickle doses. Mice from each strain were divided into larval intensity groupings based upon the number of larvae recovered from their brain. Activity for each mouse was measured pre- and post-infection by observing its behaviour in the home cage. Activity was assessed by monitoring six different independent categories of murine behaviour – ambulation, grooming, rearing, digging, climbing and immobility. Within each behavioural category, the duration of time spent at each behaviour per mouse within one thousandth of a second, the number of short bouts performed and the number of long bouts of behaviour performed were recorded over a 20 min period. Activity of LACA and NIH mice differed prior to infection. LACA mice spent more time immobile compared to NIH mice, which ambulated and climbed more. Variations in activity were also observed between groups of mice prior to infection. The effect of infection differed by strain, by dose and by larval intensity. Post-infection LACA mice became more immobile and ambulated less. NIH mice showed reduced immobility, but while ambulation decreased digging and climbing increased post-infection. Short bouts of activity remained unchanged among LACA mice post-infection but showed an increase for some behaviours in NIH mice.

Home cage activity and behavioral performance in inbred and hybrid mice

2002 ◽  
Vol 136 (2) ◽  
pp. 555-569 ◽  
Author(s):  
Xiangdong Tang ◽  
Stuart M Orchard ◽  
Larry D Sanford
Keyword(s):  
Home Cage ◽  
Behavioral Performance ◽  
Cage Activity ◽  
Home Cage Activity ◽  
Hybrid Mice

Genetic study of multigenic factors related to strain difference of temporal pattern in mouse home-cage activity

2011 ◽  
Vol 71 ◽  
pp. e166
Author(s):  
Tatsuhiko Goto ◽  
Ayako Ishii ◽  
Akinori Nishi ◽  
Aki Takahashi ◽  
Toshihiko Shiroishi ◽  
...  
Keyword(s):  
Genetic Study ◽  
Temporal Pattern ◽  
Home Cage ◽  
Strain Difference ◽  
Cage Activity ◽  
Home Cage Activity
Sign in / Sign up

Export Citation Format

Share Document