Oil Immersed Lossless Total Analysis System (OIL-TAS): Integrated RNA Extraction and Detection for SARS-CoV-2 Testing

AbstractThe coronavirus disease 2019 (COVID-19) pandemic exposed difficulties in scaling current quantitative PCR (qPCR)-based diagnostic methodologies for large-scale infectious disease testing. Bottlenecks include the lengthy multi-step process of nucleic acid extraction followed by qPCR readouts, which require costly instrumentation and infrastructure, as well as reagent and plastic consumable shortages stemming from supply chain constraints. Here we report a novel Oil Immersed Lossless Total Analysis System (OIL-TAS), which integrates RNA extraction and detection onto a single device that is simple, rapid, cost effective, uses minimal supplies and requires reduced infrastructure to perform. We validated the performance of OIL-TAS using contrived samples containing inactivated SARS-CoV-2 viral particles, which show that the assay can reliably detect an input concentration of 10 copies/μL and sporadically detect down to 1 copy/μL. The OIL-TAS method can serve as a faster, cheaper, and easier-to-deploy alternative to current qPCR-based methods for infectious disease testing.

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Oil immersed lossless total analysis system for integrated RNA extraction and detection of SARS-CoV-2

Nature Communications ◽

10.1038/s41467-021-24463-4 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Duane S. Juang ◽

Terry D. Juang ◽

Dawn M. Dudley ◽

Christina M. Newman ◽

Molly A. Accola ◽

...

Keyword(s):

Infectious Disease ◽

Large Scale ◽

Rna Extraction ◽

Clinical Samples ◽

Nasopharyngeal Swab ◽

Acid Extraction ◽

Total Analysis System ◽

Total Analysis ◽

Analysis System ◽

Disease Testing

AbstractThe COVID-19 pandemic exposed difficulties in scaling current quantitative PCR (qPCR)-based diagnostic methodologies for large-scale infectious disease testing. Bottlenecks include lengthy multi-step processes for nucleic acid extraction followed by qPCR readouts, which require costly instrumentation and infrastructure, as well as reagent and plastic consumable shortages stemming from supply chain constraints. Here we report an Oil Immersed Lossless Total Analysis System (OIL-TAS), which integrates RNA extraction and detection onto a single device that is simple, rapid, cost effective, and requires minimal supplies and infrastructure to perform. We validated the performance of OIL-TAS using contrived SARS-CoV-2 viral particle samples and clinical nasopharyngeal swab samples. OIL-TAS showed a 93% positive predictive agreement (n = 57) and 100% negative predictive agreement (n = 10) with clinical SARS-CoV-2 qPCR assays in testing clinical samples, highlighting its potential to be a faster, cheaper, and easier-to-deploy alternative for infectious disease testing.

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Micro Total Analysis System (μTAS)

Encyclopedia of Microfluidics and Nanofluidics ◽

10.1007/978-0-387-48998-8_1024 ◽

2008 ◽

pp. 1359-1359

Keyword(s):

Micro Total Analysis System ◽

Total Analysis System ◽

Total Analysis ◽

Analysis System

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A Concept for a Sensitive Micro Total Analysis System for High Throughput Fluorescence Imaging

Sensors ◽

10.3390/s6040341 ◽

2006 ◽

Vol 6 (4) ◽

pp. 341-349 ◽

Cited By ~ 4

Author(s):

Arthur Rabner ◽

Yosi Shacham

Keyword(s):

Fluorescence Imaging ◽

High Throughput ◽

Micro Total Analysis System ◽

Total Analysis System ◽

Total Analysis ◽

Analysis System

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Detection of Directivity of Fluorescence From a Mixed Specimen Which Consists of Micro Particles Attached Different Fluorescent Substances Using a Light Waveguide Incorporated Optical TAS

ASME-JSME 2018 Joint International Conference on Information Storage and Processing Systems and Micromechatronics for Information and Precision Equipment ◽

10.1115/isps-mipe2018-8503 ◽

2018 ◽

Author(s):

Toshifumi Ohkubo ◽

Nobuyuki Terada ◽

Yoshikazu Yoshida

Keyword(s):

Microfluidic Channel ◽

Laser Source ◽

Microfluidic Channels ◽

Fluorescent Substance ◽

Total Analysis System ◽

Micro Particles ◽

Total Analysis ◽

Light Waveguide ◽

Analysis System ◽

Near Future

A resin-based optical total analysis system (O-TAS) which consists both of microfluidic channels and light waveguides [1] is thought to be one of the most promising components in developing a “ubiquitous human healthcare system” in the near future. Along with this technology trend, we have already developed a transparent epoxy-resin-based optical TAS chip which has a specially prepared light waveguide structure of radially arranged configuration at an intersection portion with a microfluidic channel, in order to detect directivity of fluorescence from fluorescent substance attached micro particles [2],[3]. Schematic diagram of the optical TAS is shown in Figure 1. In the latest research, utilizing an AC modulated laser source and time-series averaging function on detected signal waveforms, we could have successfully obtained directivities of fluorescence from 5-μm-diameter particles with higher signal to noise (S/N) ratio [3].

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Micro Particle Imaging Velocimetry Measurements in High Aspect Ratio Passive Microfluidic Components

ASME 3rd International Conference on Microchannels and Minichannels, Part B cont’d ◽

10.1115/icmm2005-75137 ◽

2005 ◽

Author(s):

Paul Chiarot ◽

Pierre Sullivan ◽

Ridha Ben Mrad

Keyword(s):

Aspect Ratio ◽

High Aspect Ratio ◽

Particle Imaging Velocimetry ◽

Passive Component ◽

Total Analysis System ◽

Micro Piv ◽

Micro Total Analysis Systems ◽

Total Analysis ◽

Analysis System ◽

Particle Imaging

In this work, micro particle imaging velocimetry (micro-PIV) was performed on the fundamental components of a micro total analysis system. Specifically, high aspect ratio passive valves and mixers were designed, fabricated, and characterized. The components were built using Micralyne Protolyne technology on a glass substrate and operated at reasonably achievable pressures. The flows through the components were analyzed both qualitatively and quantitatively with the goal of developing a more complete understanding of internal device performance. Using the results of the micro-PIV developed velocity fields it was found that the high aspect ratio passive valves are able to perform at reasonably achievable pressures. However, it was determined that the high aspect ratio passive mixers offer limited performance enhancements because of the low Reynolds number flows. The results of this work contribute to the understanding of passive component operation and address some of the challenges associated with developing completely integrated micro total analysis systems that use passive devices.

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Miniaturization of fluorescence sensing in optofluidic devices

Microfluidics and Nanofluidics ◽

10.1007/s10404-020-02371-1 ◽

2020 ◽

Vol 24 (9) ◽

Cited By ~ 1

Author(s):

Daniel Măriuţa ◽

Stéphane Colin ◽

Christine Barrot-Lattes ◽

Stéphane Le Calvé ◽

Jan G. Korvink ◽

...

Keyword(s):

Lab On A Chip ◽

Time Data ◽

Fluorescence Sensing ◽

Successful Development ◽

Total Analysis System ◽

Total Analysis ◽

Integration Strategies ◽

Analysis System ◽

On Chip ◽

Industrial Sensing

Abstract Successful development of a micro-total-analysis system (µTAS, lab-on-a-chip) is strictly related to the degree of miniaturization, integration, autonomy, sensitivity, selectivity, and repeatability of its detector. Fluorescence sensing is an optical detection method used for a large variety of biological and chemical assays, and its full integration within lab-on-a-chip devices remains a challenge. Important achievements were reported during the last few years, including improvements of previously reported methodologies, as well as new integration strategies. However, a universal paradigm remains elusive. This review considers achievements in the field of fluorescence sensing miniaturization, starting from off-chip approaches, representing miniaturized versions of their lab counter-parts, continuing gradually with strategies that aim to fully integrate fluorescence detection on-chip, and reporting the results around integration strategies based on optical-fiber-based designs, optical layer integrated designs, CMOS-based fluorescence sensing, and organic electronics. Further successful development in this field would enable the implementation of sensing networks in specific environments that, when coupled to Internet-of-Things (IoT) and artificial intelligence (AI), could provide real-time data collection and, therefore, revolutionize fields like health, environmental, and industrial sensing.

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