Detection of Directivity of Fluorescence From a Mixed Specimen Which Consists of Micro Particles Attached Different Fluorescent Substances Using a Light Waveguide Incorporated Optical TAS

2018 ◽  
Author(s):  
Toshifumi Ohkubo ◽  
Nobuyuki Terada ◽  
Yoshikazu Yoshida
Keyword(s):  
Microfluidic Channel ◽  
Laser Source ◽  
Microfluidic Channels ◽  
Fluorescent Substance ◽  
Total Analysis System ◽  
Micro Particles ◽  
Total Analysis ◽  
Light Waveguide ◽  
Analysis System ◽  
Near Future

A resin-based optical total analysis system (O-TAS) which consists both of microfluidic channels and light waveguides [1] is thought to be one of the most promising components in developing a “ubiquitous human healthcare system” in the near future. Along with this technology trend, we have already developed a transparent epoxy-resin-based optical TAS chip which has a specially prepared light waveguide structure of radially arranged configuration at an intersection portion with a microfluidic channel, in order to detect directivity of fluorescence from fluorescent substance attached micro particles [2],[3]. Schematic diagram of the optical TAS is shown in Figure 1. In the latest research, utilizing an AC modulated laser source and time-series averaging function on detected signal waveforms, we could have successfully obtained directivities of fluorescence from 5-μm-diameter particles with higher signal to noise (S/N) ratio [3].

Evaluation of Fluorescence Emitting Characteristics of a Micro Particle by Illumination Angle Scanning Utilizing a Resin-Based Monolithic TAS Chip

2016 ◽  
Author(s):  
Toshifumi Ohkubo ◽  
Nobuyuki Terada ◽  
Yoshikazu Yoshida
Keyword(s):  
Laser Power ◽  
Microfluidic Channel ◽  
Experimental System ◽  
Fluorescent Substance ◽  
Scanning Method ◽  
Total Analysis System ◽  
Illumination Direction ◽  
Micro Particles ◽  
Light Waveguide ◽  
Functional Components

It has long been thought that an optical sensor, such as a light waveguide implemented total analysis system (TAS), is one of the functional components that will be needed to realize a “ubiquitous human healthcare system” in the near future. We have already proposed the fundamental structure for a light waveguide capable of illuminating a living cell or particle running along a microfluidic channel, as well as of detecting fluorescence even from the extremely weak power of such a minute particle. In order to develop novel functions to detect the internal structure of living cells conveniently, an angular scanning method that sequentially changes the direction of illumination of the minute cell or particle may be crucial. In this research paper, we investigated fluorescence detection from moving resin particles by switching direction of laser power illumination by radially arranged outlet portions of several light waveguides, as a step-by-step examination toward this novel scanning method. To cost effectively construct an experimental system able to incorporate a mechanism of switching illumination direction, we utilized a monolithic resin-based TAS chip[1] with plural waveguide pairs whose outlet portions were arranged radially and inlet portions were arranged in parallel, and a forced vibration mechanism on an optical fiber tip by a piezoelectric actuator. With this chip and system, we constructed an experimental system to detect extremely weak fluorescence using micro particles with a fluorescent substance attached and an optical TAS chip that incorporated a microfluidic channel and five pairs of laser-power-delivering light waveguide.

Illumination and Scattered Light Detection of a Minute Particle Using a Light Source Consisting of Sub-Micrometer Defect Arrays Formed on an Extremely Flat Light Waveguide Core

2013 ◽  
Author(s):  
Toshifumi Ohkubo ◽  
Nobuyuki Terada ◽  
Yoshikazu Yoshida
Keyword(s):  
Light Source ◽  
Optical Fibers ◽  
Laser Power ◽  
Scattered Light ◽  
Total Analysis System ◽  
Physiological Properties ◽  
Visible Laser ◽  
Total Analysis ◽  
Light Waveguide ◽  
Analysis System

A minute optical sensor combined total analysis system (TAS) is thought to be one of the most powerful functional elements needed to realize a “ubiquitous human healthcare” system. In accordance with this concept, we have proposed a fundamental structure of detecting forward and side scattered light from a small cell or particle illuminating laser power through light waveguide formed in a thin resin layer. Based on this concept, we have demonstrated its effectiveness by using a trial-manufactured optical TAS chip, supplying and detecting visible laser power by using multiple optical fibers. Acquiring further various physiological properties created by an internal structure of a particles or cells, it is necessary to illuminate them with a small and arbitrarily-shaped light source placed close vicinity to them.

Fluorescence Detection of a Minute Particle Using Resin-Based Optical Total Analysis Systems Having a High Aspect Ratio Light Waveguide Core

2014 ◽  
Author(s):  
Toshifumi Ohkubo ◽  
Nobuyuki Terada ◽  
Yoshikazu Yoshida
Keyword(s):  
Scattered Light ◽  
Small Particles ◽  
Ubiquitous Healthcare ◽  
Total Analysis System ◽  
Physiological Properties ◽  
Section Size ◽  
Plane Direction ◽  
Total Analysis ◽  
Light Waveguide ◽  
Analysis System

A precise light waveguide combined total analysis system (TAS)[1] is thought to be one of the most powerful functional elements needed to realize a “ubiquitous healthcare” system. In accordance with this concept, we have proposed a fundamental structure of detecting forward and side scattered light from small particles illuminating laser power through light waveguide formed in a thin resin layer. Based on this concept, we demonstrated its effectiveness of detecting both forward and side scattered light in in-plane direction[2] (Fig.1) by using a trial-manufactured TAS chip. Acquiring further various physiological properties, it is necessary to illuminate them with smaller cross section size of cores of light waveguide, and furthermore, to construct fluorescence detecting systems.

Micro Particle Imaging Velocimetry Measurements in High Aspect Ratio Passive Microfluidic Components

2005 ◽  
Author(s):  
Paul Chiarot ◽  
Pierre Sullivan ◽  
Ridha Ben Mrad
Keyword(s):  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Particle Imaging Velocimetry ◽  
Passive Component ◽  
Total Analysis System ◽  
Micro Piv ◽  
Micro Total Analysis Systems ◽  
Total Analysis ◽  
Analysis System ◽  
Particle Imaging

In this work, micro particle imaging velocimetry (micro-PIV) was performed on the fundamental components of a micro total analysis system. Specifically, high aspect ratio passive valves and mixers were designed, fabricated, and characterized. The components were built using Micralyne Protolyne technology on a glass substrate and operated at reasonably achievable pressures. The flows through the components were analyzed both qualitatively and quantitatively with the goal of developing a more complete understanding of internal device performance. Using the results of the micro-PIV developed velocity fields it was found that the high aspect ratio passive valves are able to perform at reasonably achievable pressures. However, it was determined that the high aspect ratio passive mixers offer limited performance enhancements because of the low Reynolds number flows. The results of this work contribute to the understanding of passive component operation and address some of the challenges associated with developing completely integrated micro total analysis systems that use passive devices.

scholarly journals Miniaturization of fluorescence sensing in optofluidic devices

2020 ◽  
Vol 24 (9) ◽  
Author(s):  
Daniel Măriuţa ◽  
Stéphane Colin ◽  
Christine Barrot-Lattes ◽  
Stéphane Le Calvé ◽  
Jan G. Korvink ◽  
...  
Keyword(s):  
Lab On A Chip ◽  
Time Data ◽  
Fluorescence Sensing ◽  
Successful Development ◽  
Total Analysis System ◽  
Total Analysis ◽  
Integration Strategies ◽  
Analysis System ◽  
On Chip ◽  
Industrial Sensing

Abstract Successful development of a micro-total-analysis system (µTAS, lab-on-a-chip) is strictly related to the degree of miniaturization, integration, autonomy, sensitivity, selectivity, and repeatability of its detector. Fluorescence sensing is an optical detection method used for a large variety of biological and chemical assays, and its full integration within lab-on-a-chip devices remains a challenge. Important achievements were reported during the last few years, including improvements of previously reported methodologies, as well as new integration strategies. However, a universal paradigm remains elusive. This review considers achievements in the field of fluorescence sensing miniaturization, starting from off-chip approaches, representing miniaturized versions of their lab counter-parts, continuing gradually with strategies that aim to fully integrate fluorescence detection on-chip, and reporting the results around integration strategies based on optical-fiber-based designs, optical layer integrated designs, CMOS-based fluorescence sensing, and organic electronics. Further successful development in this field would enable the implementation of sensing networks in specific environments that, when coupled to Internet-of-Things (IoT) and artificial intelligence (AI), could provide real-time data collection and, therefore, revolutionize fields like health, environmental, and industrial sensing.

Water glass bonding for micro-total analysis system

2002 ◽  
Vol 81 (2-3) ◽  
pp. 187-195 ◽  
Author(s):  
Takeshi Ito ◽  
Kazuharu Sobue ◽  
Seishiro Ohya
Keyword(s):  
Water Glass ◽  
Micro Total Analysis System ◽  
Total Analysis System ◽  
Total Analysis ◽  
Analysis System

A SU-8 microfluidic total analysis system integrating silicon photodiodes and buried waveguides

2005 ◽  
Author(s):  
P. de la Fuente ◽  
J.A. Etxeberria ◽  
J. Berganzo ◽  
M.T. Arroyo ◽  
E. Castano ◽  
...  
Keyword(s):  
Total Analysis System ◽  
Total Analysis ◽  
Analysis System
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