scholarly journals The pseudouridine synthase dyskerin binds to cytoplasmic H/ACA-box snoRNA retaining transcripts affecting nuclear hormone receptor dependence

2021 ◽  
Author(s):  
Federico Zacchini ◽  
Giulia Venturi ◽  
Veronica De Sanctis ◽  
Roberto Bertorelli ◽  
Claudio Ceccarelli ◽  
...  

ABSTRACTDyskerin is a nuclear protein involved in H/ACA box snoRNA-guided uridine modification of RNA. Since its defective function induces specific alterations in gene expression, we sought to unbiasedly identify mRNAs regulated by dyskerin. We found that dyskerin depletion affects the expression or the association with polysomes of selected mRNA isoforms characterized by the retention of H/ACA box snoRNA-containing introns. These snoRNA retaining transcripts (snoRTs) are bound by dyskerin and can interact with cytoplasmic ribosomes. We then characterized the cytoplasmic dyskerin RNA interactome finding both H/ACA box snoRTs and protein-coding transcripts. Since a fraction of these latter transcripts is involved in the nuclear hormone receptor binding, we tested to see if this specific activity is affected by dyskerin. Results indicate that dyskerin dysregulation may alter the dependence on nuclear hormone receptor ligands in breast cancer. Our work suggests a cytoplasmic function for dyskerin which could affect mRNA post-transcriptional networks relevant for nuclear hormone receptor functions.

Development ◽  
1998 ◽  
Vol 125 (9) ◽  
pp. 1617-1626 ◽  
Author(s):  
M. Kostrouchova ◽  
M. Krause ◽  
Z. Kostrouch ◽  
J.E. Rall

CHR3 is a Caenorhabditis elegans orphan nuclear hormone receptor highly homologous to Drosophila DHR3, an ecdysone-inducible gene product involved in metamorphosis. Related vertebrate factors include RORalpha/RZRalpha, RZRbeta and RevErb. Gel-shift studies show that CHR3 can bind the DR5-type hormone response sequence. CHR3 is a nuclear protein present in all blastomeres during early embryogenesis. During morphogenesis, both CHR3 protein and zygotically active reporter genes are detectable in epidermal cells and their precursors. Inhibition of the gene encoding CHR3 results in several larval defects associated with abnormal epidermal cell function, including molting and body size regulation, suggesting that CHR3 is an essential epidermal factor required for proper postembryonic development.


2009 ◽  
Vol 284 (12) ◽  
pp. 7542-7552 ◽  
Author(s):  
Shivani Garapaty ◽  
Chong-Feng Xu ◽  
Patrick Trojer ◽  
Muktar A. Mahajan ◽  
Thomas A. Neubert ◽  
...  

2019 ◽  
Author(s):  
Kathryn M. Appleton ◽  
Mi-Hye Lee ◽  
Erik G. Strungs ◽  
Carlos Nogueras-Ortiz ◽  
Diane Gesty-Palmer ◽  
...  

2007 ◽  
Vol 17 (7) ◽  
pp. 2018-2021 ◽  
Author(s):  
Johan Malm ◽  
Sandra Gordon ◽  
Peter Brandt ◽  
Bo Carlsson ◽  
Peter Agback ◽  
...  

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