Thriving of hyperthermophilic microbial communities from a deep-sea sulfidic hydrothermal chimney under electrolithoautotrophic conditions with nitrate as electron acceptor

AbstractRecent studies have shown the presence of an abiotic electrical current across the walls of deep-sea hydrothermal chimneys, allowing the growth of electroautotrophic microbial communities. To understand the role of the different phylogenetic groups and metabolisms involved, this study focused on an electrotrophic enrichment, with nitrate as electron acceptor. The biofilm density, the community composition, the organic products released, and the electrical consumption were monitored by FISH confocal microscopy, qPCR, Metabarcoding, MNR and potentiostat measurements. A statistic analysis by PCA showed the correlation between the different parameters in 3 distinct temporal phases. The Archaeoglobales have been shown to play a key role in the development of the community, as first colonizers and producing pyruvate, therefor used as organic source for heterotrophs. Some Thermococcales showed the ability to perform electrofermentation of this pyruvate into acetate and H2. Finally, through subcultures of the community, we showed the development of a larger biodiversity over time. This observed phenomenon could explain the biodiversity development in hydrothermal context where energy sources are transient and unstable.

Download Full-text

Evolution of Thermophilic Microbial Communities from a Deep-Sea Hydrothermal Chimney under Electrolithoautotrophic Conditions with Nitrate

Microorganisms ◽

10.3390/microorganisms9122475 ◽

2021 ◽

Vol 9 (12) ◽

pp. 2475

Author(s):

Guillaume Pillot ◽

Oulfat Amin Ali ◽

Sylvain Davidson ◽

Laetitia Shintu ◽

Yannick Combet-Blanc ◽

...

Keyword(s):

Microbial Communities ◽

Organic Compounds ◽

Deep Sea ◽

Electrical Current ◽

Electron Acceptors ◽

Energy Sources ◽

Phylogenetic Groups ◽

Electrical Consumption ◽

Over Time

Recent studies have shown the presence of an abiotic electrical current across the walls of deep-sea hydrothermal chimneys, allowing the growth of electroautotrophic microbial communities. To understand the role of the different phylogenetic groups and metabolisms involved, this study focused on electrotrophic enrichment with nitrate as electron acceptor. The biofilm density, community composition, production of organic compounds, and electrical consumption were monitored by FISH confocal microscopy, qPCR, metabarcoding, NMR, and potentiostat measurements. A statistical analysis by PCA showed the correlation between the different parameters (qPCR, organic compounds, and electron acceptors) in three distinct temporal phases. In our conditions, the Archaeoglobales have been shown to play a key role in the development of the community as the first colonizers on the cathode and the first producers of organic compounds, which are then used as an organic source by heterotrophs. Finally, through subcultures of the community, we showed the development of a greater biodiversity over time. This observed phenomenon could explain the biodiversity development in hydrothermal contexts, where energy sources are transient and unstable.

Download Full-text

Disturbance and recovery: a synthesis of microbial community reassembly following disturbance across realms

10.22541/au.163818947.78737764/v1 ◽

2021 ◽

Author(s):

Stephanie Jurburg ◽

Shane Blowes ◽

Ashley Shade ◽

Nico Eisenhauer ◽

Jonathan Chase

Keyword(s):

Time Series ◽

Microbial Community ◽

Microbial Communities ◽

Community Composition ◽

Community Assembly ◽

Disturbance And Recovery ◽

Microbial Community Assembly ◽

Community Reassembly ◽

Over Time

Disturbances alter the diversity and composition of microbial communities, but whether microbiomes from different environments exhibit similar degrees of resistance or rates of recovery has not been evaluated. Here, we synthesized 86 time series of disturbed mammalian, aquatic, and soil microbiomes to examine how the recovery of microbial richness and community composition differed after disturbance. We found no general patterns in compositional variance (i.e., dispersion) in any microbiomes over time. Only mammalian microbiomes consistently exhibited decreases in richness following disturbance. Importantly, they tended to recover this richness, but not their composition, over time. In contrast, aquatic microbiomes tended to diverge from their pre-disturbance composition following disturbance. By synthesizing microbiome responses across environments, our study aids in the reconciliation of disparate microbial community assembly frameworks, and highlights the role of the environment in microbial community reassembly following disturbance.

Download Full-text

Fluctuations in populations of subsurface methane oxidizers in coordination with changes in electron acceptor availability

10.1101/040204 ◽

2016 ◽

Cited By ~ 1

Author(s):

C. Magnabosco ◽

P.H.A. Timmers ◽

M.C.Y. Lau ◽

G. Borgonie ◽

B. Linage-Alvarez ◽

...

Keyword(s):

Microbial Communities ◽

Electron Acceptor ◽

Land Surface ◽

Community Change ◽

Electron Donors ◽

Geochemical Composition ◽

Incubation Experiments ◽

Cellular Turnover ◽

Terrestrial Subsurface ◽

Over Time

AbstractThe concentrations of electron donors and acceptors in the terrestrial subsurface biosphere fluctuate due to migration and mixing of subsurface fluids, but the mechanisms and rates at which microbial communities respond to these changes are largely unknown. Subsurface microbial communities exhibit long cellular turnover times and are often considered relatively static—generating just enough ATP for cellular maintenance. Here, we investigated how subsurface populations of CH4oxidizers respond to changes in electron acceptor availability by monitoring the biological and geochemical composition in a 1,339 meters-below-land-surface (mbls) fluid-filled fracture over the course of both longer (2.5 year) and shorter (2-week) time scales. Using a combination of metagenomic, metatranscriptomic, and metaproteomic analyses, we observe that the CH4oxidizers within the subsurface microbial community change in coordination with electron acceptor availability over time. We then validate these findings through a series of13C-CH4laboratory incubation experiments, highlighting a connection between composition of subsurface CH4oxidizing communities and electron acceptor availability.

Download Full-text

The role of neighborhood risk factors and personal stressors in romantic partner support and relationship quality among African American couples over time

PsycEXTRA Dataset ◽

10.1037/e512142015-577 ◽

2014 ◽

Author(s):

Frederick D. Clavel ◽

Carolyn E. Cutrona ◽

Daniel W. Russell

Keyword(s):

Risk Factors ◽

African American ◽

Relationship Quality ◽

Partner Support ◽

Romantic Partner ◽

Neighborhood Risk ◽

African American Couples ◽

Over Time

Download Full-text

The Role of Computer Modeling in Electrocardiography

Methods of Information in Medicine ◽

10.1055/s-0038-1634809 ◽

1990 ◽

Vol 29 (04) ◽

pp. 282-288 ◽

Cited By ~ 2

Author(s):

A. van Oosterom

Keyword(s):

Electrical Activity ◽

Computer Modeling ◽

Body Surface ◽

Electrical Current ◽

The Body ◽

Volume Conductor ◽

Current Generator ◽

Cardiac Electrical Activity ◽

Source Models

AbstractThis paper introduces some levels at which the computer has been incorporated in the research into the basis of electrocardiography. The emphasis lies on the modeling of the heart as an electrical current generator and of the properties of the body as a volume conductor, both playing a major role in the shaping of the electrocardiographic waveforms recorded at the body surface. It is claimed that the Forward-Problem of electrocardiography is no longer a problem. Several source models of cardiac electrical activity are considered, one of which can be directly interpreted in terms of the underlying electrophysiology (the depolarization sequence of the ventricles). The importance of using tailored rather than textbook geometry in inverse procedures is stressed.

Download Full-text

SISTEM AUTOMATIC SWITCH REDUNDANT UPS UNTUK BEBAN ESSENSIAL

Electro Luceat ◽

10.32531/jelekn.v5i1.144 ◽

2019 ◽

Vol 5 (1) ◽

pp. 35-45

Author(s):

Markus Dwiyanto Tobi ◽

Alimuddin Mappa

Keyword(s):

Power Supply ◽

Working Condition ◽

Control Circuit ◽

Energy Sources ◽

Main Equipment ◽

Telecommunication Equipment ◽

Input Condition ◽

Research Designs ◽

Switch Circuit

The role of the power supply device is to produce, process and distribute energy sources. Telecommunication equipment can only operate if it has continuous supply. Therefore, to maintain the continuity of the supply, a UPS (Uninterruptable Power Supply) device system is needed so that the supply to the Essential Load device will remain available so that continuity will be maintained. This research designs and proposes how a series of automatic redundant switch systems on UPS to ensure the availability of power supply for the main equipment of telecommunications systems. The Auto switch circuit is designed to have 3 (three) working stages which will trigger the relay driver as control circuit, namely the normal working condition of the contactor input K1 is present, the input condition is zero (lost), and the input condition is present. This system can automatically supply power to telecommunications equipment.

Download Full-text