scholarly journals OCT2Hist: Non-Invasive Virtual Biopsy Using Optical Coherence Tomography

Author(s):  
Yonatan Winetraub ◽  
Edwin Yuan ◽  
Itamar Terem ◽  
Caroline Yu ◽  
Warren Chan ◽  
...  

Histological haematoxylin and eosin–stained (H&E) tissue sections are used as the gold standard for pathologic detection of cancer, tumour margin detection, and disease diagnosis1. Producing H&E sections, however, is invasive and time-consuming. Non-invasive optical imaging modalities, such as optical coherence tomography (OCT), permit label-free, micron-scale 3D imaging of biological tissue microstructure with significant depth (up to 1mm) and large fields-of-view2, but are difficult to interpret and correlate with clinical ground truth without specialized training3. Here we introduce the concept of a virtual biopsy, using generative neural networks to synthesize virtual H&E sections from OCT images. To do so we have developed a novel technique, “optical barcoding”, which has allowed us to repeatedly extract the 2D OCT slice from a 3D OCT volume that corresponds to a given H&E tissue section, with very high alignment precision down to 25 microns. Using 1,005 prospectively collected human skin sections from Mohs surgery operations of 71 patients, we constructed the largest dataset of H&E images and their corresponding precisely aligned OCT images, and trained a conditional generative adversarial network4 on these image pairs. Our results demonstrate the ability to use OCT images to generate high-fidelity virtual H&E sections and entire 3D H&E volumes. Applying this trained neural network to in vivo OCT images should enable physicians to readily incorporate OCT imaging into their clinical practice, reducing the number of unnecessary biopsy procedures.

2021 ◽  
Vol 137 ◽  
pp. 106861
Author(s):  
Deepa Joshi ◽  
Ankit Butola ◽  
Sheetal Raosaheb Kanade ◽  
Dilip K. Prasad ◽  
S.V. Amitha Mithra ◽  
...  

2020 ◽  
Vol 49 (2) ◽  
pp. 20190071
Author(s):  
Dario Di Stasio ◽  
Dorina Lauritano ◽  
Francesca Loffredo ◽  
Enrica Gentile ◽  
Fedora Della Vella ◽  
...  

Objectives: Optical coherence tomography (OCT) is a non-invasive technique based on optical imaging with a micrometre resolution. The purpose of this study is to investigate the potential role of OCT in evaluating oral mucosa bullous diseases. Methods: two patients with bullous pemphigoid (BP) and one patient with pemphigus vulgaris (PV) were examined and images of their oral lesions were performed using OCT. Results: In OCT images, the BP blister has a clearly different morphology from the PV one compared to the blistering level. Conclusion: This exploratory study suggests that the OCT is able to distinguish epithelial and subepithelial layer in vivo images of healthy oral mucosa from those with bullous diseases, assisting the clinicians in differential diagnosis.The presented data are in accordance with the scientific literature, although a wider pool of cases is needed to increase statistical power. Histological examination and immunofluorescence methods remain the gold standard for the diagnosis of oral bullous diseases. In this context, the OCT can provide the clinician with a valuable aid both as an additional diagnostic tool and in the follow up of the disease.


2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Cedric Blatter ◽  
Eelco F. J. Meijer ◽  
Ahhyun S. Nam ◽  
Dennis Jones ◽  
Brett E. Bouma ◽  
...  

2016 ◽  
Vol 7 (12) ◽  
pp. 4886 ◽  
Author(s):  
Peijun Gong ◽  
Shaghayegh Es’haghian ◽  
Karl-Anton Harms ◽  
Alexandra Murray ◽  
Suzanne Rea ◽  
...  

Author(s):  
Simon Brais-Brunet ◽  
Udayakumar Kanniyappan ◽  
Hamid Hosseiny ◽  
Emilie Heckel ◽  
Jean-Sebastien Joyal ◽  
...  

2011 ◽  
Vol 91 (11) ◽  
pp. 1596-1604 ◽  
Author(s):  
Jeremiah Wierwille ◽  
Peter M Andrews ◽  
Maristela L Onozato ◽  
James Jiang ◽  
Alex Cable ◽  
...  

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