scholarly journals Optical coherence tomography imaging of oral mucosa bullous diseases: a preliminary study

2020 ◽  
Vol 49 (2) ◽  
pp. 20190071
Author(s):  
Dario Di Stasio ◽  
Dorina Lauritano ◽  
Francesca Loffredo ◽  
Enrica Gentile ◽  
Fedora Della Vella ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Oral Mucosa ◽  
Statistical Power ◽  
Pemphigus Vulgaris ◽  
Invasive Technique ◽  
Optical Coherence ◽  
Non Invasive ◽  
Bullous Diseases ◽  
Subepithelial Layer

Objectives: Optical coherence tomography (OCT) is a non-invasive technique based on optical imaging with a micrometre resolution. The purpose of this study is to investigate the potential role of OCT in evaluating oral mucosa bullous diseases. Methods: two patients with bullous pemphigoid (BP) and one patient with pemphigus vulgaris (PV) were examined and images of their oral lesions were performed using OCT. Results: In OCT images, the BP blister has a clearly different morphology from the PV one compared to the blistering level. Conclusion: This exploratory study suggests that the OCT is able to distinguish epithelial and subepithelial layer in vivo images of healthy oral mucosa from those with bullous diseases, assisting the clinicians in differential diagnosis.The presented data are in accordance with the scientific literature, although a wider pool of cases is needed to increase statistical power. Histological examination and immunofluorescence methods remain the gold standard for the diagnosis of oral bullous diseases. In this context, the OCT can provide the clinician with a valuable aid both as an additional diagnostic tool and in the follow up of the disease.

Development of Instrumentation and Methods to Image the Retina in Mouse Pup with in vivo Non-Invasive Optical Coherence Tomography

2019 ◽  
Author(s):  
Simon Brais-Brunet ◽  
Udayakumar Kanniyappan ◽  
Hamid Hosseiny ◽  
Emilie Heckel ◽  
Jean-Sebastien Joyal ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Non Invasive ◽  
Mouse Pup

scholarly journals Biospeckle Optical Coherence Tomography (bOCT) in the Speedy Assessment of the Responses of the Seeds of Raphanus sativus L. (Kaiware Daikon) to Acid Mine Drainage (AMD)

2021 ◽  
Vol 12 (1) ◽  
pp. 355
Author(s):  
Danyang Li ◽  
Uma Maheswari Rajagopalan ◽  
Y. Sanath K. De Silva ◽  
Fenwu Liu ◽  
Hirofumi Kadono
Keyword(s):  
Optical Coherence Tomography ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Mineral Resources ◽  
Vital Role ◽  
Invasive Technique ◽  
Optical Coherence ◽  
Non Invasive ◽  
Acid Mine ◽  
Short Time

The extraction of mineral resources from mines plays a vital role in global socio-economic development. However, acid mine drainage (AMD) has been one of the major pollutants, and a vast area of the agricultural fields has been polluted. Therefore, techniques for monitoring the response of plants to AMD that arise during mineral extraction are necessary. In addition, such a technique becomes especially valuable to understand how the plants could play a role in the phytoremediation of AMD. We propose the use of biospeckle Optical Coherence Tomography (bOCT) to investigate the response of Kaiware daikon seeds under the exposure to simulated AMD at two different concentrations of 40 mL/L and 80 mL/L. OCT images of the Kaiware daikon seed were obtained at a speed of 10 frames per second (1 frame: 512 × 2048 pixels) for a few tens of seconds. For each pixel of the OCT structural images, the contrast across the temporal axis was calculated to give biospeckle contrast OCT images (bOCT images). It was found that bOCT images clearly distinguished the changes due to 40 mL/L and 80 mL/L of AMD treatments from the control within a short time of around an hour, compared to the conventional OCT images that failed to show any changes. This variation was found to be statistically significant and could reflect the internal activity of the seeds. The proposed bOCT method could be a rapid, non-invasive technique for screening suitable plants in AMD phytoremediation applications.

scholarly journals Optical coherence tomography: a non-invasive technique applied to conservation of paintings

2005 ◽  
Author(s):  
Haida Liang ◽  
Marta Gomez Cid ◽  
Radu Cucu ◽  
George Dobre ◽  
Boris Kudimov ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Invasive Technique ◽  
Optical Coherence ◽  
Non Invasive

scholarly journals Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography

Micromachines ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 35 ◽  
Author(s):  
Meng-Tsan Tsai ◽  
Bo-Huei Huang ◽  
Chun-Chih Yeh ◽  
Kin Fong Lei ◽  
Ngan-Ming Tsang
Keyword(s):  
Optical Coherence Tomography ◽  
Cancer Cell ◽  
Cellular Response ◽  
Tumor Development ◽  
Three Dimensional ◽  
Cancer Cell Line ◽  
Optical Coherence ◽  
Non Invasive

Investigation of tumor development is essential in cancer research. In the laboratory, living cell culture is a standard bio-technology for studying cellular response under tested conditions to predict in vivo cellular response. In particular, the colony formation assay has become a standard experiment for characterizing the tumor development in vitro. However, quantification of the growth of cell colonies under a microscope is difficult because they are suspended in a three-dimensional environment. Thus, optical coherence tomography (OCT) imaging was develop in this study to monitor the growth of cell colonies. Cancer cell line of Huh 7 was used and the cells were applied on a layer of agarose hydrogel, i.e., a non-adherent surface. Then, cell colonies were gradually formed on the surface. The OCT technique was used to scan the cell colonies every day to obtain quantitative data for describing their growth. The results revealed the average volume increased with time due to the formation of cell colonies day-by-day. Additionally, the distribution of cell colony volume was analyzed to show the detailed information of the growth of the cell colonies. In summary, the OCT provides a non-invasive quantification technique for monitoring the growth of the cell colonies. From the OCT images, objective and precise information is obtained for higher prediction of the in vivo tumor development.

scholarly journals In Vivo Endoscopic Optical Coherence Tomography of the Healthy Human Oral Mucosa: Qualitative and Quantitative Image Analysis

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 827
Author(s):  
Marius Albrecht ◽  
Christian Schnabel ◽  
Juliane Mueller ◽  
Jonas Golde ◽  
Edmund Koch ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Image Analysis ◽  
Soft Tissue ◽  
Oral Mucosa ◽  
Cross Sections ◽  
Optical Coherence ◽  
Healthy Human ◽  
Quantitative Image ◽  
Endoscopic Optical Coherence Tomography

To date, there is still a lack of reliable imaging modalities to improve the quality of consultation, diagnostic and medical examinations of the oral mucosa in dentistry. Even though, optical technologies have become an important element for the detection and treatment of different diseases of soft tissue, for the case of oral screenings the evidence of the benefit in comparison to conventional histopathology is mostly still pending. One promising optical technology for oral diagnostics is optical coherence tomography (OCT). To prove the potential of OCT, even the amount of freely accessible OCT data is not sufficient to describe the variance of healthy human oral soft tissue in vivo. In order to remedy this deficiency, the present study provides in vivo OCT cross sections of the human oral mucosa of the anterior and posterior oral cavity as well as the oropharynx of 47 adult volunteers. A collection of representative OCT cross sections forms the basis for a randomized blinded image analysis by means of seven criteria to assess the main features of the superficial layers of the human oral mucosa and to determine its correlation to regional features known from hematoxylin and eosin (HE) stained histology.

scholarly journals 2-D Kasai velocity estimation for Doppler optical coherence tomography / by Darren Morofke.

2021 ◽  
Author(s):  
Darren Morofke
Keyword(s):  
Blood Flow ◽  
Optical Coherence Tomography ◽  
Frequency Estimation ◽  
Doppler Frequency ◽  
Velocity Estimation ◽  
Low Flow ◽  
Invasive Technique ◽  
Optical Coherence ◽  
Detection Range

Optical Coherence Tomography (OCT) is a high-resolution, non-invasive technique to image subsurface tissue and tissue functions. A broadband light source illuminates an object and the reflected photons are processed using an interferometer, demodulated into inphase and quadrature components and then digitized. The captured data contains information about the velocity of the moving scatterers but current Doppler estimation algorithms have a limited velocity detection range. Here we demonstrate Doppler OCT (DOCT) detection of in vivo of blood flow in a rat aorta with over 1 m/s peak velocity through an esophageal DOCT probe using a new processing technique. Previous methods have used a transverse Kasai (TK) autocorrelation estimation to estimate the velocity. By calculating the Kasai autocorrelation with a lag in the depth or axial direction, backscattered frequency information is obtained. Through subtraction with stationary backscattered information, the Doppler shift is obtained by the axial Kasai (AK) technique. Maximum non-aliased Doppler frequency estimation using a time domain DOCT system increased from +/-4 kHz to =+/-1.6 MHz. The TK has better velocity resolution in the low flow rate range and when combined with the AK we demonstrate a dynamic frequency range over 100 dB with a velocity detection range from 10 [micro]m/s to over 1 m/s. This velocity range spans from microcirculation to cardiac blood flow velocities.

scholarly journals Pachychoroid as a biomarker using optical coherence tomography – swept-source in central serous choroidopathy

2019 ◽  
Vol 2 ◽  
pp. 3
Author(s):  
René Alfredo Cano-Hidalgo ◽  
Tatiana Urrea-Victoria
Keyword(s):  
Optical Coherence Tomography ◽  
Choroidal Thickness ◽  
Invasive Technique ◽  
Optical Coherence ◽  
Horizontal Section ◽  
Swept Source ◽  
Non Invasive ◽  
The Mean ◽  
Foveal Center ◽  
Fellow Eyes

Objective The objective of the study was to describe and evaluate the subfoveal choroidal thickness (SFCT) in the fellow eyes of patients with central serous chorioretinopathy (CSC) using swept source optical coherence tomography (SS-OCT). Methods This was a transversal, retrospective, and observational study. The SFCT was measured in patients with unilateral CSC using SS-OCT. The choroidal thickness in symptomatic and fellow eyes was measured using the attached measuring software in SS-OCT. The SFCT dimension was obtained from the horizontal section under the foveal center from the OCT data and these data were analyzed. Results The mean age of subjects undergoing imaging SS-OCT was 44.23 years old (standard deviation, 11.57). 30 out of 60 patients (63.3%) were men, and 20 (33.3%) patients had acute clinical disease. The median choroidal thicknesses of the affected eyes were greater than those of the unaffected fellow eyes (P = 0.06). The choroidal thickness measured in 120 eyes of (60 patients) was 421 µm (interquartile range 352–490), which was greater than the choroidal thickness reported in normal eyes. Conclusions The measuring of the choroidal thickness using SS-OCT is useful as a non-invasive technique to evaluate the subclinical choroidal abnormalities in CSC.

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