scholarly journals Ethanol pre-exposure enhances alcohol-seeking behavior at cellular level by chemoattraction and exhibits bleb-driven cellular stress response in uniform ethanol concentration

2021 ◽  
Author(s):  
Neelakshi Kar ◽  
Jayesh Bellare
Keyword(s):  
Ethanol Concentration ◽  
Cellular Level ◽  
Cellular Stress Response ◽  
Migratory Behavior ◽  
Cell Protection ◽  
Significant Difference ◽  
Seeking Behavior ◽  
Concentration Cells ◽  
Alcohol Seeking ◽  
Alcohol Dependent

This study investigates the effect of ethanol and its pre-exposure on cell migration. Here, fibroblast cells were first pre-treated with ethanol, and their migratory behavior was tested in both chemotaxis and chemokinesis setup. 1% ethanol was taken as a potential chemotactic agent. The study reveals that in presence of ethanol gradient cells display migration towards ethanol, and pre-exposure further augments this migratory behavior by altering their chemotactic responsiveness. In uniform ethanol concentration, cells first undergo three staged adaptations to the new environment: shrinking, blebbing, and recovery, where cells use bleb-driven cell protection machinery to adapt. Thus, migration is initially stalled. But once the cells resume locomotion, no significant difference in migratory parameters is observed. Overall, this study establishes ethanol as a chemoattractant for fibroblasts, with cells showing enhanced alcohol-seeking behavior upon pre-exposure. Such behavior is reminiscent of seeking and tolerance exhibited by alcohol-dependent addictive behavior in higher organisms including humans.

scholarly journals Matrix Metalloproteinase-9 and Synaptic Plasticity in the Central Amygdala in Control of Alcohol-Seeking Behavior

2017 ◽  
Vol 81 (11) ◽  
pp. 907-917 ◽  
Author(s):  
Marzena Stefaniuk ◽  
Anna Beroun ◽  
Tomasz Lebitko ◽  
Olga Markina ◽  
Szymon Leski ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 9 ◽  
Central Amygdala ◽  
Seeking Behavior ◽  
Metalloproteinase 9 ◽  
Alcohol Seeking

Baclofen attenuates cue-induced reinstatement of alcohol-seeking behavior in Sardinian alcohol-preferring (sP) rats

2008 ◽  
Vol 95 (3) ◽  
pp. 284-287 ◽  
Author(s):  
Paola Maccioni ◽  
Przemyslaw Bienkowski ◽  
Mauro A.M. Carai ◽  
Gian Luigi Gessa ◽  
Giancarlo Colombo
Keyword(s):  
Seeking Behavior ◽  
Alcohol Seeking

scholarly journals Mountain stoneflies may tolerate warming streams: evidence from organismal physiology and gene expression

2019 ◽  
Author(s):  
Scott Hotaling ◽  
Alisha A. Shah ◽  
Kerry L. McGowan ◽  
Lusha M. Tronstad ◽  
J. Joseph Giersch ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Constitutive Expression ◽  
High Elevation ◽  
Cellular Level ◽  
Cellular Stress Response ◽  
Mountain Stream ◽  
Sequencing Data ◽  
Stream Insects ◽  
Short Term Exposure ◽  
Genes Encoding

AbstractRapid glacier recession is altering the physical conditions of headwater streams. Stream temperatures are predicted to rise and become increasingly variable, putting entire meltwater-associated biological communities at risk of extinction. Thus, there is a pressing need to understand how thermal stress affects mountain stream insects, particularly where glaciers are likely to vanish on contemporary timescales. In this study, we tested the critical thermal maximum (CTMAX) of stonefly nymphs representing multiple species and a range of thermal regimes in the high Rocky Mountains, USA. We then collected RNA-sequencing data to assess how organismal thermal stress translated to the cellular level. Our focal species included the meltwater stonefly, Lednia tumana, which was recently listed under the U.S. Endangered Species Act due to climate-induced habitat loss. For all study species, critical thermal maxima (CTMAX > 20°C) far exceeded the stream temperatures mountain stoneflies experience (< 10°C). Moreover, while evidence for a cellular stress response was present, we also observed constitutive expression of genes encoding proteins known to underlie thermal stress (i.e., heat shock proteins) even at low temperatures that reflected natural conditions. We show that high-elevation aquatic insects may not be physiologically threatened by short-term exposure to warm temperatures and that longer term physiological responses or biotic factors (e.g., competition) may better explain their extreme distributions.

scholarly journals Targeted overexpression of CRH receptor subtype 1 in central amygdala neurons: effect on alcohol-seeking behavior

2018 ◽  
Vol 235 (6) ◽  
pp. 1821-1833 ◽  
Author(s):  
L. Broccoli ◽  
S. Uhrig ◽  
G. von Jonquieres ◽  
K. Schönig ◽  
D. Bartsch ◽  
...  
Keyword(s):  
Receptor Subtype ◽  
Central Amygdala ◽  
Seeking Behavior ◽  
Alcohol Seeking

scholarly journals Prefrontal Cortex K Ca 2 Channels Regulate mGlu 5 -Dependent Plasticity and Extinction of Alcohol-Seeking Behavior

2017 ◽  
Vol 37 (16) ◽  
pp. 4359-4369 ◽  
Author(s):  
Reginald Cannady ◽  
Justin T. McGonigal ◽  
Ryan J. Newsom ◽  
John J. Woodward ◽  
Patrick J. Mulholland ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Dependent Plasticity ◽  
Seeking Behavior ◽  
Alcohol Seeking

3D culture model and computer-assisted videomicroscopy to analyze migratory behavior of noninvasive and invasive bronchial epithelial cells

2005 ◽  
Vol 289 (6) ◽  
pp. C1547-C1552 ◽  
Author(s):  
Salma Hazgui ◽  
Noël Bonnet ◽  
Jérôme Cutrona ◽  
Béatrice Nawrocki-Raby ◽  
Myriam Polette ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Line ◽  
3D Model ◽  
Migratory Behavior ◽  
Computer Assisted ◽  
Data Set ◽  
Significant Difference ◽  
Kinetics Of ◽  
Invasive Cell

To date, most of the studies in the field of cell migration have been applied to two-dimensional (2D) models. To mimic the three-dimensional (3D) conditions similar to those observed in vivo during tumor invasion, we developed a 3D model of cell migration in which cells were embedded in a collagen I matrix placed in a double-compartment chamber. Using time-lapse videomicroscopy and interactive cell tracking in a four-dimensional data set, we determined the cell trajectories and their migration kinetics. We compared the 2D and 3D migratory behavior of a noninvasive cell line (16HBE) with the migratory behavior of an invasive cell line (BZR). Our results show that the 3D migration kinetics of the noninvasive cell line were lower than the migration kinetics of the invasive cell line. In contrast, in 2D models, no significant difference was observed between the two cell lines. To validate our 3D model, we further investigated the effect of epidermal growth factor (EGF), a promoter of tumor cell motility and invasion on the noninvasive cell line (16HBE). EGF increased significantly the migration kinetics of the noninvasive cell line. Our results show that the 3D model of cell migration allowed us to differentiate the migratory behavior of invasive and noninvasive cells and that such a model can help in the development of molecular targeted therapy as it approaches the in vivo conditions.

The role of corticotrophin-releasing factor in stress-induced relapse to alcohol-seeking behavior in rats

2000 ◽  
Vol 150 (3) ◽  
pp. 317-324 ◽  
Author(s):  
A. D. Lê ◽  
S. Harding ◽  
W. Juzytsch ◽  
J. Watchus ◽  
U. Shalev ◽  
...  
Keyword(s):  
Corticotrophin Releasing Factor ◽  
Seeking Behavior ◽  
Alcohol Seeking
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