Using ultraviolet absorption spectroscopy to study nanoswitches based on non-canonical DNA structures

AbstractNon-canonical forms of DNA are attracting increasing interest for applications in nanotechnology. It is frequently convenient to characterize DNA molecules using a label-free approach such as UV absorption spectroscopy. In this paper we present the results of our investigation into the use of this technique to probe the folding of quadruplex and triplex nanoswitches. We confirmed that four G-quartets were necessary for folding at sub-mM concentrations of potassium and found that the wrong choice of sequence for the linker between G-tracts could dramatically disrupt folding, presumably due to the presence of kinetic traps in the folding landscape. In the case of the triplex nanoswitch we examined, we found that the UV spectrum showed a small change in absorbance when a triplex was formed. We anticipate that our results will be of interest to researchers seeking to design DNA nanoswitches based on quadruplexes and triplexes.

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Ultraviolet Absorption Spectroscopy in the Study of Vulcanization

Rubber Chemistry and Technology ◽

10.5254/1.3540364 ◽

1964 ◽

Vol 37 (3) ◽

pp. 709-713 ◽

Cited By ~ 2

Author(s):

D. W. Huke ◽

C. E. Kendall

Keyword(s):

Thin Films ◽

Absorption Spectroscopy ◽

Uv Absorption ◽

Low Molecular Weight ◽

Research Association ◽

Model Compounds ◽

Uv Absorption Spectroscopy ◽

Steric Factors ◽

Normal Procedure ◽

Ultraviolet Absorption Spectroscopy

Abstract Practically all UV absorption spectroscopy is done in solution, and examples of its use in examination of neat (one component) solid materials are relatively few. In the IR on the other hand, the examination of thin films and microtomed sections of elastomers and plastics is a normal procedure. The reasons for this are fairly obvious: scatter by optically imperfect films is much more serious in the UV, many polymers give no specific UV absorption, and those that do absorb often give such intense absorption that impracticably thin films would be required. The main value of UV is in examination of trace amounts of UV-absorbing materials in those polymers which are more or less transparent, the best example being the study of crosslinking of elastomers. This work cannot be done in solution because the cross-linked polymers are insoluble in all solvents. A great deal of the work on the chemistry of vulcanization has therefore been done on model compounds of low molecular weight, most notably at the Natural Rubber Producers' Research Association. Although the use of model olefins has yielded valuable information, investigations on polymers are required because the different conditions, particularly in steric factors, may appreciably alter the balance of reactions.

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Simulation of UV Absorption Spectra and Relaxation Dynamics of Uracil and Uracil-Water Clusters

Physical Chemistry Chemical Physics ◽

10.1039/d0cp05618a ◽

2020 ◽

Author(s):

Branislav Milovanović ◽

Jurica Novak ◽

Mihajlo Etinski ◽

Wolfgang Domcke ◽

Nadja Doslic

Keyword(s):

Aqueous Solution ◽

Gas Phase ◽

Absorption Spectra ◽

Absorption Spectroscopy ◽

Water Clusters ◽

Uv Absorption ◽

Relaxation Dynamics ◽

Nonradiative Relaxation ◽

Uv Absorption Spectra ◽

Uv Absorption Spectroscopy

Despite many studies, the mechanisms of nonradiative relaxation of uracil in the gas phase and in aqueous solution are still not fully resolved. Here we combine theoretical UV absorption spectroscopy...

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The synthesis of polymeric dyes based on waterborne polyurethane: a reaction kinetics study using UV absorption spectroscopy

New Journal of Chemistry ◽

10.1039/c9nj05490a ◽

2020 ◽

Vol 44 (7) ◽

pp. 2930-2940 ◽

Cited By ~ 1

Author(s):

Jun Zhu ◽

Jie Li ◽

Chao Sang ◽

Yunjun Luo

Keyword(s):

Reaction Kinetics ◽

Absorption Spectroscopy ◽

Waterborne Polyurethane ◽

Uv Absorption ◽

Kinetics Study ◽

Uv Absorption Spectroscopy ◽

First Time ◽

Polymeric Dyes

The preparation of waterborne polyurethane based polymeric dyes (DWPU) with anthraquinone chromophores was monitored by using UV absorption spectroscopy for the first time.

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