FGF signalling is involved in cumulus migration in the common house spider Parasteatoda tepidariorum

Cell migration is a fundamental component during the development of most multicellular organisms. In spiders, the collective migration of a signalling centre, known as the cumulus, is required to set the dorsoventral body axis of the embryo. Here, we show that FGF signalling plays an important role during cumulus migration in the spider Parasteatoda tepidariorum. Spider embryos with reduced FGF signalling lack cumulus migration and display dorsoventral patterning defects. Our study reveals that cumulus expression of several FGF signalling components is regulated by the transcription factor Ets4. In conjunction with a previous study, we show that the expression of fgf8 in the germ-disc is regulated via the Hedgehog signalling pathway. We also demonstrate that FGF signalling influences the BMP signalling pathway activity in the region around cumulus cells. Finally, we show that FGF signalling might also influence cumulus migration in basally branching spiders and we propose a hypothetical model in which fgf8 acts a chemo-attractant to guide cumulus cells towards the future dorsal pole of the spider embryo.

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The common house spider Parasteatoda tepidariorum

EvoDevo ◽

10.1186/s13227-020-00152-z ◽

2020 ◽

Vol 11 (1) ◽

Cited By ~ 4

Author(s):

Hiroki Oda ◽

Yasuko Akiyama-Oda

Keyword(s):

Parasteatoda Tepidariorum ◽

The Common ◽

House Spider ◽

Common House

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The common house spider, Parasteatoda tepidariorum, maintains silk gene expression on sub-optimal diet

10.1101/2020.07.27.222638 ◽

2020 ◽

Author(s):

Jeremy Miller ◽

Jannelle Vienneau-Hathaway ◽

Enkhbileg Dendev ◽

Merrina Lan ◽

Nadia A. Ayoub

Keyword(s):

Gene Expression ◽

Amino Acid ◽

Expression Patterns ◽

High Energy ◽

Fiber Types ◽

Parasteatoda Tepidariorum ◽

Gene Transcripts ◽

The Common ◽

House Spider ◽

Common House

AbstractOrb-web weaving spiders and their relatives spin multiple task-specific fiber types. The unique material properties of each silk type result from differences in amino acid sequence and structure of their component proteins, primarily spidroins (spider fibrous proteins). Amino acid content and gene expression measurements of spider silks suggest some spiders change expression patterns of individual protein components in response to environmental cues. We quantified mRNA abundance of three spidroin encoding genes involved in prey capture in the common house spider, Parasteatoda tepidariorum (Theridiidae), fed different diets. After 10 days of acclimation to the lab on a diet of mealworms, spiders were split into three groups: (1) individuals were immediately dissected, (2) spiders were fed high-energy crickets, or (3) spiders were fed low-energy flies, for 1 month. All spiders gained mass during the acclimation period and cricket-fed spiders continued to gain mass, while fly fed spiders either maintained or lost mass. Using quantitative PCR, we found no significant differences in the absolute or relative abundance of dragline gene transcripts, major ampullate spidroin 1 (MaSp1) and major ampullate spidroin 2 (MaSp2), among groups. In contrast, prey-wrapping minor ampullate spidroin (MiSp) gene transcripts were significantly less abundant in fly-fed than lab-acclimated spiders. However, when measured relative to actin, cricket-fed spiders showed the lowest expression of MiSp. Our results suggest that house spiders are able to maintain silk production, even in the face of a low-quality diet.

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Molecular characterization and embryonic origin of the eyes in the common house spider Parasteatoda tepidariorum

EvoDevo ◽

10.1186/s13227-015-0011-9 ◽

2015 ◽

Vol 6 (1) ◽

Cited By ~ 23

Author(s):

Christoph Schomburg ◽

Natascha Turetzek ◽

Magdalena Ines Schacht ◽

Julia Schneider ◽

Phillipp Kirfel ◽

...

Keyword(s):

Molecular Characterization ◽

Parasteatoda Tepidariorum ◽

Embryonic Origin ◽

The Common ◽

House Spider ◽

Common House

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Microarray data on the comparison of transcript expression between normal and Pt-Delta RNAi embryos in the common house spider Parasteatoda tepidariorum

Data in Brief ◽

10.1016/j.dib.2019.104350 ◽

2019 ◽

Vol 25 ◽

pp. 104350

Author(s):

Hiroki Oda ◽

Yasuko Akiyama-Oda

Keyword(s):

Microarray Data ◽

Transcript Expression ◽

Parasteatoda Tepidariorum ◽

The Common ◽

House Spider ◽

Common House

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A Comprehensive Reference Transcriptome Resource for the Common House Spider Parasteatoda tepidariorum

PLoS ONE ◽

10.1371/journal.pone.0104885 ◽

2014 ◽

Vol 9 (8) ◽

pp. e104885 ◽

Cited By ~ 38

Author(s):

Nico Posnien ◽

Victor Zeng ◽

Evelyn E. Schwager ◽

Matthias Pechmann ◽

Maarten Hilbrant ◽

...

Keyword(s):

Reference Transcriptome ◽

Parasteatoda Tepidariorum ◽

The Common ◽

Transcriptome Resource ◽

House Spider ◽

Common House ◽

Comprehensive Reference

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Common House Spider, Achaearanea tepidariorum (C. L. Koch) (Arachnida: Araneae: Theridiidae)

EDIS ◽

10.32473/edis-in394-2001 ◽

1969 ◽

Vol 2004 (8) ◽

Author(s):

Glavis B. Edwards

Keyword(s):

United States ◽

Cooperative Extension ◽

Southeastern United States ◽

Cooperative Extension Service ◽

Extension Service ◽

University Of Florida ◽

Parasteatoda Tepidariorum ◽

The Common ◽

House Spider ◽

Common House

The common house spider, Achaearanea tepidariorum (C.L. Koch), may be the most abundant of the several species of spiders that live in the company of man in the southeastern United States, especially in Florida. Although Archer (1947) thought that A. tepidariorum was less common inside houses than Pholcus phalangioides Fuesslin (Pholcidae) in Alabama, he also noted its abundance. This document is EENY-238 (originally published as DPI Entomology Circular 279), one of a series of Featured Creatures from the Entomology and Nematology Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Published: September 2001. EENY-238/IN394: The Common House Spider, Parasteatoda tepidariorum (C. L. Koch) (Arachnida: Araneae: Theridiidae) (ufl.edu)

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The common house spider, Parasteatoda tepidariorum, maintains silk gene expression on sub-optimal diet

PLoS ONE ◽

10.1371/journal.pone.0237286 ◽

2020 ◽

Vol 15 (12) ◽

pp. e0237286

Author(s):

Jeremy Miller ◽

Jannelle Vienneau-Hathaway ◽

Enkhbileg Dendev ◽

Merrina Lan ◽

Nadia A. Ayoub

Keyword(s):

Gene Expression ◽

Amino Acid ◽

Expression Patterns ◽

High Energy ◽

Fiber Types ◽

Parasteatoda Tepidariorum ◽

Gene Transcripts ◽

The Common ◽

House Spider ◽

Common House

Cobweb weaving spiders and their relatives spin multiple task-specific fiber types. The unique material properties of each silk type result from differences in amino acid sequence and structure of their component proteins, primarily spidroins (spider fibrous proteins). Amino acid content and gene expression measurements of spider silks suggest some spiders change expression patterns of individual protein components in response to environmental cues. We quantified mRNA abundance of three spidroin encoding genes involved in prey capture in the common house spider, Parasteatoda tepidariorum (Theridiidae), fed different diets. After 10 days of acclimation to the lab on a diet of mealworms, spiders were split into three groups: (1) individuals were immediately dissected, (2) spiders were fed high-energy crickets, or (3) spiders were fed low-energy flies, for 1 month. All spiders gained mass during the acclimation period and cricket-fed spiders continued to gain mass, while fly-fed spiders either maintained or lost mass. Using quantitative PCR, we found no significant differences in the absolute or relative abundance of dragline gene transcripts, major ampullate spidroin 1 (MaSp1) and major ampullate spidroin 2 (MaSp2), among groups. In contrast, prey-wrapping minor ampullate spidroin (MiSp) gene transcripts were significantly less abundant in fly-fed than lab-acclimated spiders. However, when measured relative to Actin, cricket-fed spiders showed the lowest expression of MiSp. Our results suggest that house spiders are able to maintain silk production, even in the face of a low-quality diet.

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