Ultrahigh-Throughput Direct Sampling MS: Sampling at 22 Hz by Infrared Matrix-Assisted Desorption Electrospray Ionization Mass Spectrometry
Infrared Matrix-Assisted Desorption Electrospray Ionization (IR-MALDESI) mass spectrometry is an ambient-direct sampling method being developed for high-throughput, label-free, biochemical screening of large-scale compound libraries. Here, we report the development of an ultrahigh-throughput continuous motion IR-MALDESI sampling approach capable of acquiring data at rates up to 22.7 samples per second in a 384-well microtiter plate. At top speed, less than 1% analyte carryover is observed from well-to-well and intensity relative standard deviations (RSD) of 11.5% and 20.9% for 3 micromolar 1-hydroxymidazolam and 12 micromolar dextrorphan, respectively, are achieved. The ability to perform parallel kinetics studies on 384 samples with ~30s time resolution using an isocitrate dehydrogenase 1 (IDH1) enzyme assay is shown. Taken together, these results demonstrate the use of IR-MALDESI at sample acquisition rates that surpass other currently reported direct sampling mass spectrometry approaches.