scholarly journals A quasi-integral controller for adaptation of genetic modules to variable ribosome demand

2018 ◽  
Author(s):  
Hsin-Ho Huang ◽  
Yili Qian ◽  
Domitilla Del Vecchio
Keyword(s):  
Gene Expression ◽  
Small Rna ◽  
Sigma Factor ◽  
Feedback Controller ◽  
Gene Expression Level ◽  
Expression Level ◽  
Modular Construction ◽  
Genetic Circuits ◽  
Ecf Sigma Factor ◽  
Integral Controller

AbstractThe behavior of genetic circuits is often poorly predictable. A gene’s expression level is not only determined by the intended regulators, but also largely dictated by changes in ribosome availability imparted by activation or repression of other genes. To address this problem, we design a quasi-integral biomolecular feedback controller that enables the expression level of any gene of interest (GOI) to adapt to changes in available ribosomes. The feedback is implemented through a synthetic small RNA (sRNA) that silences the GOI’s mRNA, and uses orthogonal extracytoplasmic function (ECF) sigma factor to sense the GOI’s translation and to actuate sRNA transcription. Without the controller, the expression level of the GOI is reduced by 50% when a resource competitor is activated. With the controller, by contrast, gene expression level is practically unaffected by the competitor. This feedback controller allows adaptation of genetic modules to variable ribosome demand and thus aids modular construction of complicated circuits.

Gene Expression Level Analysis

2020 ◽  
Author(s):  
Keyword(s):  
Gene Expression ◽  
Gene Expression Level ◽  
Expression Level ◽  
Level Analysis

scholarly journals Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level

1999 ◽  
Vol 179 (2) ◽  
pp. 367-373 ◽  
Author(s):  
Vibhor Saraswat ◽  
Dae Young Kim ◽  
Jeewon Lee ◽  
Young-Hoon Park
Keyword(s):  
Gene Expression ◽  
Recombinant Protein ◽  
Production Rate ◽  
Plasmid Vector ◽  
Gene Expression Level ◽  
Expression Level ◽  
Specific Production ◽  
Specific Production Rate

The investigation of CD36 scavenger receptor and MSR1 scavenger receptor gene expression level in patients with atherosclerosis

2010 ◽  
Vol 27 ◽  
pp. S66
Author(s):  
M. Piechota ◽  
A. Banaszewska ◽  
E. Guzniczak ◽  
G. Rosinski ◽  
T. Siminiak ◽  
...  
Keyword(s):  
Gene Expression ◽  
Receptor Gene ◽  
Scavenger Receptor ◽  
Gene Expression Level ◽  
Expression Level ◽  
Receptor Gene Expression

SAT0005 Human C-Type Lectin Domain Family 4, Member C Gene Expression Level Helps Predict Future Clinical Response to Tabalumab Blockade of Baff in Rheumatoid Arthritis

2013 ◽  
Vol 72 (Suppl 3) ◽  
pp. A580.2-A580
Author(s):  
E. R. Dow ◽  
P. Banerjee ◽  
M. A. Penny ◽  
E. Nantz ◽  
S. Stepaniants ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Gene Expression ◽  
Clinical Response ◽  
Gene Expression Level ◽  
Expression Level ◽  
Domain Family ◽  
Lectin Domain

The impact of gene-body H3K36me3 patterns on gene expression level changes in chronic myelogenous leukemia

Gene ◽  
2021 ◽  
pp. 145862
Author(s):  
Lu-Qiang Zhang ◽  
Jun-Jie Liu ◽  
Li Liu ◽  
Guo-Liang Fan ◽  
Yan-Nan Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chronic Myelogenous Leukemia ◽  
Myelogenous Leukemia ◽  
Gene Expression Level ◽  
Expression Level ◽  
Gene Body ◽  
The Impact

scholarly journals Elevated Lipocalin-2 Can Indicate the Vascular Inflammation in Patients with Ischemic Stroke

2019 ◽  
Author(s):  
Rajnics P ◽  
◽  
Kellner A ◽  
Nagy F ◽  
Alföldi V ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ischemic Stroke ◽  
Gene Expression Level ◽  
Expression Level ◽  
Neutrophil Granulocytes ◽  
Lipocalin 2 ◽  
Relative Gene Expression ◽  
Thrombotic Risk ◽  
Relative Gene ◽  
Relative Gene Expression Level

Purpose: Elevated level of Lipocalin-2 (LCN2), a new acute phase adipokine, was described after ischemic stroke. A number of researchers feel as though that LCN2 originated from the infiltrating neutrophils and other cells in brain after stroke. Others measured elevated LCN2 expression in arteriosclerotic plaque. Therefore we have investigated LCN2 relative gene expression level of blood neutrophil granulocytes in patients with ischemic stroke to assess if elevated LCN2 is the cause or consequence of ischemic stroke. Methods: Laboratory and anamnestic data were collected, which could have a role in development of thrombo-embolic events in patients with ischemic stroke. RNA based method was used to evaluate the relative gene expression level of LCN2. We calculated Odds Ratio (OR) and Confidence Interval (CI) for the association between LCN2 and ischemic stroke. Results: 34 samples were available for evaluation. The LCN 2 relative gene expression level was decreased in 12 cases. In this group, 91% of patients have Atrial Fibrillation (AF) at the time of hospitalisation. The mean LCN2 relative gene expression value was 64.25% (ranges: 34%-115%) in patients with AF. It was significantly lower than in patients with normal sinus rhythm (409.2%; ranges: 127%-1127%; p=0.0003). The elevated LCN2 relative gene expression level significantly (p=0.012) increases the risk of stroke (OR: 12.6) independently from other factors. Conclusions: High LCN2 expression level seems to have strong positive predictive value on ischemic stroke, and may be useful in thrombotic risk stratification of plaque vulnerability in these patients.

Sequence determinants of prokaryotic gene expression level under heat stress

Gene ◽  
2014 ◽  
Vol 551 (1) ◽  
pp. 92-102 ◽  
Author(s):  
Heng Xiong ◽  
Yi Yang ◽  
Xiao-Pan Hu ◽  
Yi-Ming He ◽  
Bin-Guang Ma
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Gene Expression Level ◽  
Expression Level

scholarly journals Analyzing and Tuning Ribozyme Activity by Deep Sequencing To Modulate Gene Expression Level in Mammalian Cells

2018 ◽  
Vol 7 (2) ◽  
pp. 371-376 ◽  
Author(s):  
Shungo Kobori ◽  
Yohei Yokobayashi
Keyword(s):  
Gene Expression ◽  
Deep Sequencing ◽  
Mammalian Cells ◽  
Gene Expression Level ◽  
Expression Level
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