scholarly journals Digital removal of autofluorescence from microscopy images

2019 ◽  
Author(s):  
Heeva Baharlou ◽  
Nicolas P Canete ◽  
Kirstie M Bertram ◽  
Kerrie J Sandgren ◽  
Anthony L Cunningham ◽  
...  
Keyword(s):  
Image Analysis ◽  
Fluorescence Microscopy ◽  
Protein Expression ◽  
Supplementary Information ◽  
Link Type ◽  
Microscopy Image ◽  
Microscopy Image Analysis ◽  
Microscopy Images ◽  
Standing Problem

AbstractAutofluorescence is a long-standing problem that has hindered fluorescence microscopy image analysis. To address this, we have developed a method that identifies and removes autofluorescent signals from multi-channel images post acquisition. We demonstrate the broad utility of this algorithm in accurately assessing protein expression in situ through the removal of interfering autofluorescent signals.Availability and implementationhttps://ellispatrick.github.io/[email protected] informationSupplementary Figs. 1–13

AFid: a tool for automated identification and exclusion of autofluorescent objects from microscopy images

2020 ◽  
Author(s):  
Heeva Baharlou ◽  
Nicolas P Canete ◽  
Kirstie M Bertram ◽  
Kerrie J Sandgren ◽  
Anthony L Cunningham ◽  
...  
Keyword(s):  
Image Analysis ◽  
Fluorescence Microscopy ◽  
Hiv Transmission ◽  
Supplementary Information ◽  
Supplementary Data ◽  
Automated Identification ◽  
Immune Markers ◽  
Downstream Analysis ◽  
Microscopy Images ◽  
Standing Problem

Abstract Motivation Autofluorescence is a long-standing problem that has hindered the analysis of images of tissues acquired by fluorescence microscopy. Current approaches to mitigate autofluorescence in tissue are lab-based and involve either chemical treatment of sections or specialized instrumentation and software to ‘unmix’ autofluorescent signals. Importantly, these approaches are pre-emptive and there are currently no methods to deal with autofluorescence in acquired fluorescence microscopy images. Results To address this, we developed Autofluorescence Identifier (AFid). AFid identifies autofluorescent pixels as discrete objects in multi-channel images post-acquisition. These objects can then be tagged for exclusion from downstream analysis. We validated AFid using images of FFPE human colorectal tissue stained for common immune markers. Further, we demonstrate its utility for image analysis where its implementation allows the accurate measurement of HIV–Dendritic cell interactions in a colorectal explant model of HIV transmission. Therefore, AFid represents a major leap forward in the extraction of useful data from images plagued by autofluorescence by offering an approach that is easily incorporated into existing workflows and that can be used with various samples, staining panels and image acquisition methods. We have implemented AFid in ImageJ, Matlab and R to accommodate the diverse image analysis community. Availability and implementation AFid software is available at https://ellispatrick.github.io/AFid. Supplementary information Supplementary data are available at Bioinformatics online.

scholarly journals Rotation equivariant and invariant neural networks for microscopy image analysis

2019 ◽  
Vol 35 (14) ◽  
pp. i530-i537 ◽  
Author(s):  
Benjamin Chidester ◽  
Tianming Zhou ◽  
Minh N Do ◽  
Jian Ma
Keyword(s):  
Neural Networks ◽  
Image Analysis ◽  
High Throughput ◽  
Protein Localization ◽  
Effective Means ◽  
Image Data ◽  
Supplementary Information ◽  
Microscopy Image ◽  
Microscopy Image Analysis ◽  
Microscopy Images

Abstract Motivation Neural networks have been widely used to analyze high-throughput microscopy images. However, the performance of neural networks can be significantly improved by encoding known invariance for particular tasks. Highly relevant to the goal of automated cell phenotyping from microscopy image data is rotation invariance. Here we consider the application of two schemes for encoding rotation equivariance and invariance in a convolutional neural network, namely, the group-equivariant CNN (G-CNN), and a new architecture with simple, efficient conic convolution, for classifying microscopy images. We additionally integrate the 2D-discrete-Fourier transform (2D-DFT) as an effective means for encoding global rotational invariance. We call our new method the Conic Convolution and DFT Network (CFNet). Results We evaluated the efficacy of CFNet and G-CNN as compared to a standard CNN for several different image classification tasks, including simulated and real microscopy images of subcellular protein localization, and demonstrated improved performance. We believe CFNet has the potential to improve many high-throughput microscopy image analysis applications. Availability and implementation Source code of CFNet is available at: https://github.com/bchidest/CFNet. Supplementary information Supplementary data are available at Bioinformatics online.

scholarly journals An Advanced Framework for Time-lapse Microscopy Image Analysis

2020 ◽  
Author(s):  
Qibing Jiang ◽  
Praneeth Sudalagunta ◽  
Mark B. Meads ◽  
Khandakar Tanvir Ahmed ◽  
Tara Rutkowski ◽  
...  
Keyword(s):  
Image Analysis ◽  
Drug Response ◽  
Time Lapse ◽  
Response To Therapy ◽  
Patient Specific ◽  
Microscopy Imaging ◽  
Time Lapse Microscopy ◽  
Link Type ◽  
Microscopy Image ◽  
Microscopy Image Analysis

ABSTRACTTime-lapse microscopy is a powerful technique that generates large volumes of image-based information to quantify the behaviors of cell populations. This method has been applied to cancer studies to estimate the drug response for precision medicine and has great potential to address inter-patient (or intertumoral) heterogeneity. A couple of algorithms exist to analyze time-lapse microscopy images; however, most deal with very high-resolution images involving few cells (typically cell lines). There are currently no advanced and efficient computational frameworks available to process large-scale time-lapse microscopy imaging data to estimate patient-specific response to therapy based on a large population of primary cells. In this paper, we propose a robust and user-friendly pipeline to preprocess the images and track the behaviors of thousands of cancer cells simultaneously for a better drug response prediction of cancer patients.Availability and ImplementationSource code is available at: https://github.com/CompbioLabUCF/CellTrackACM Reference FormatQibing Jiang, Praneeth Sudalagunta, Mark B. Meads, Khandakar Tanvir Ahmed, Tara Rutkowski, Ken Shain, Ariosto S. Silva, and Wei Zhang. 2020. An Advanced Framework for Time-lapse Microscopy Image Analysis. In Proceedings of BioKDD: 19th International Workshop on Data Mining In Bioinformatics (BioKDD). ACM, New York, NY, USA, 8 pages. https://doi.org/10.1145/nnnnnnn.nnnnnnn

scholarly journals SPOT DETECTION METHODS IN FLUORESCENCE MICROSCOPY IMAGING: A REVIEW

2018 ◽  
Vol 37 (3) ◽  
pp. 173 ◽  
Author(s):  
Matsilele Aubrey Mabaso ◽  
Daniel James Withey ◽  
Bhekisipho Twala
Keyword(s):  
Image Analysis ◽  
Fluorescence Microscopy ◽  
Research Effort ◽  
Detection Methods ◽  
Microscopy Imaging ◽  
Spot Detection ◽  
Microscopy Image ◽  
Benchmark Datasets ◽  
A Cell ◽  
Microscopy Image Analysis

Fluorescence microscopy imaging has become one of the essential tools used by biologists to visualize and study intracellular particles within a cell. Studying these particles is a long-term research effort in the field of microscopy image analysis, consisting of discovering the relationship between the dynamics of particles and their functions. However, biologists are faced with challenges such as the counting and tracking of these intracellular particles. To overcome the issues faced by biologists, tools which can extract the location and motion of these particles are essential. One of the most important steps in these analyses is to accurately detect particle positions in an image, termed spot detection. The detection of spots in microscopy imaging is seen as a critical step for further quantitative analysis. However, the evaluation of these microscopic images is mainly conducted manually, with automated methods becoming popular. This work presents some advances in fluorescence microscopy image analysis, focusing on the detection methods needed for quantifying the location of these spots. We review several existing detection methods in microscopy imaging, along with existing synthetic benchmark datasets and evaluation metrics.

Fluorescence microscopy image-analysis (FMI) for the characterization of interphase HO˙ production originated by heterogeneous catalysis

2017 ◽  
Vol 53 (17) ◽  
pp. 2575-2577 ◽  
Author(s):  
Shuo Zhang ◽  
Xie Quan ◽  
Dong Wang
Keyword(s):  
Image Analysis ◽  
Heterogeneous Catalysis ◽  
Fluorescence Microscopy ◽  
Quick Response ◽  
Microscopy Image ◽  
Response Method ◽  
Microscopy Image Analysis

The FMI visually revealed the inhomogeneity and intensity of interphase HO˙-production, performing as a quick-response method to evaluate HO˙-assigned heterogeneous catalysis.

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