scholarly journals Are the Matrix Metalloproteinases 2 and 9 beeing expressed in the mucosa of the rat small intestine during its intrauterine and postnatal life development ?

2019 ◽  
Author(s):  
Camila Audrey dos Reis ◽  
José Rosa Gomes
Keyword(s):  
Innate Immunity ◽  
Small Intestine ◽  
Mast Cells ◽  
Lamina Propria ◽  
Goblet Cells ◽  
Postnatal Life ◽  
Rat Small Intestine ◽  
The Matrix ◽  
Intestine Mucosa ◽  
Masson Trichrome Staining

ABSTRACTMMP-2 and MMP-9 are proteins with well stablished roles on the remodeling of tissue during morphogenesis. This study aimed to evaluate the activity and expression of the MMP-2 and MMP-9 in the rat small intestine mucosa layer on 15th and 18th days of intratuterine life (i.u.) and at 3rd, 10th, 17th, 25th, and 32th days after birth (a.b.). Samples were submitted to zimography, immunohistochemistry methods and Masson trichrome staining. Results showed that MMP-2 and MMP-9 were not expressed in the small intestine epithelium during intrauterine life. MMP-9 was immunolocalized in the villi goblet cells and in the lamina propria in cells identified as being the mast cells (a.b.). We concluded that in the i.u. and a.b. life the MMP-2 and MMP-9 were not expressed in the small intestine epithelium. However, after birth, because MMP-9 was expressed in the mast cels present in the lamina propria it may be involved in the remodeling process of the innate immunity that occurs during the small intestine development.

Expression of the matrix metalloproteinases 2 and 9 in the rat small intestine during intrauterine and postnatal life

2019 ◽  
Vol 303 (11) ◽  
pp. 2839-2846
Author(s):  
Camila Audrey dos Reis ◽  
Maria Albertina Miranda Soares ◽  
José Rosa Gomes
Keyword(s):  
Small Intestine ◽  
Matrix Metalloproteinases ◽  
Postnatal Life ◽  
Rat Small Intestine ◽  
The Matrix

The protease phenotype and crystalline nature of the granules of intraepithelial mast cells in Trichinella spiralis-infected mice

1995 ◽  
Vol 53 ◽  
pp. 818-819
Author(s):  
D.S. Friend ◽  
N. Ghildyal ◽  
M.F. Gurish ◽  
K.F. Austen ◽  
R.L. Stevens
Keyword(s):  
Small Intestine ◽  
Mast Cells ◽  
Epithelial Cells ◽  
Lamina Propria ◽  
Trichinella Spiralis ◽  
Intestinal Epithelial ◽  
Carboxypeptidase A ◽  
C3h Mice ◽  
Granule Morphology ◽  
Crystalline Nature

Trichinella spiralis induces a profound mastocytosis and eosinophilia in the small intestine of the infected mouse. Mouse mast cells (MC) store in their granules various combinations of at least five chymotryptic chymases [designated mouse MC protease (mMCP) 1 to 5], two tryptic proteases designated mMCP-6 and mMCP-7 and an exopeptidase, carboxypeptidase A (mMC-CPA). Using antipeptide, protease -specific antibodies to these MC granule proteases, immunohistochemistry was done to determine the distribution, number and protease phenotype of the MCs in the small intestine and spleen 10 to >60 days after Trichinella infection of BALB/c and C3H mice. TEM was performed to evaluate the granule morphology of the MCs between intestinal epithelial cells and in the lamina propria (mucosal MCs) and in the submucosa, muscle and serosa of the intestine (submucosal MCs).As noted in the table below, the number of submucosal MCs remained constant throughout the study. In contrast, on day 14, the number of MCs in the mucosa increased ~25 fold. Increased numbers of MCs were observed between epithelial cells in the mucosal crypts, in the lamina propria and to a lesser extent, between epithelial cells of the intestinal villi.

scholarly journals Quantitative Changes in the Goblet Cells of the Rat Small Intestine after Irradiation

1985 ◽  
Vol 24 (3) ◽  
pp. 291-299 ◽  
Author(s):  
A. Becciolini ◽  
D. Fabbrica ◽  
D. Cremonini ◽  
M. Balzi
Keyword(s):  
Small Intestine ◽  
Goblet Cells ◽  
Rat Small Intestine ◽  
Quantitative Changes

scholarly journals Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine

Blood ◽  
2011 ◽  
Vol 117 (1) ◽  
pp. 128-134 ◽  
Author(s):  
Mamiko Sakata-Yanagimoto ◽  
Toru Sakai ◽  
Yasuyuki Miyake ◽  
Toshiki I. Saito ◽  
Haruhiko Maruyama ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cell ◽  
Small Intestine ◽  
Mast Cells ◽  
Lamina Propria ◽  
Conditional Knockout ◽  
Small Intestinal Mucosa ◽  
Epithelial Layer ◽  
Strongyloides Venezuelensis ◽  
Small Intestinal

Abstract Notch receptor-mediated signaling is involved in the developmental process and functional modulation of lymphocytes, as well as in mast cell differentiation. Here, we investigated whether Notch signaling is required for antipathogen host defense regulated by mast cells. Mast cells were rarely found in the small intestine of wild-type C57BL/6 mice but accumulated abnormally in the lamina propria of the small-intestinal mucosa of the Notch2-conditional knockout mice in naive status. When transplanted into mast cell–deficient Wsh/Wsh mice, Notch2-null bone marrow-derived mast cells were rarely found within the epithelial layer but abnormally localized to the lamina propria, whereas control bone marrow-derived mast cells were mainly found within the epithelial layer. After the infection of Notch2 knockout and control mice with L3 larvae of Strongyloides venezuelensis, the abundant number of mast cells was rapidly mobilized to the epithelial layer in the control mice. In contrast, mast cells were massively accumulated in the lamina propria of the small intestinal mucosa in Notch2-conditional knockout mice, accompanied by impaired eradication of Strongyloides venezuelensis. These findings indicate that cell-autonomous Notch2 signaling in mast cells is required for proper localization of intestinal mast cells and further imply a critical role of Notch signaling in the host-pathogen interface in the small intestine.

scholarly journals Transient expression of [D-Ala2] deltorphin I-like immunoreactivity in prenatal rat small intestine.

1994 ◽  
Vol 42 (10) ◽  
pp. 1377-1381 ◽  
Author(s):  
J Matsui ◽  
M Fujimiya ◽  
S Matsui ◽  
Y Amakata ◽  
T Renda ◽  
...  
Keyword(s):  
Small Intestine ◽  
Electron Microscopic Examination ◽  
Goblet Cells ◽  
Postnatal Period ◽  
Intestinal Lumen ◽  
Positive Staining ◽  
Rat Small Intestine ◽  
Electron Microscopic ◽  
Apical Cytoplasm ◽  
Undifferentiated Cells

We studied the distribution of immunoreactive elements for [D-Ala2] deltorphin I (DADTI), a delta-opioid receptor ligand, in fetal and postnatal rat small intestine. DADTI-like immunoreactive cells were detected transiently on embryonic Days 20 and 21. Electron microscopic examination revealed that positive staining occurred in mucous epithelial cells, either mature goblet cells or undifferentiated cells containing only a few mucous granules. Positive immunoreaction products in mature goblet cells were confined in their apical cytoplasm to the luminal parts of mucous granule aggregates. The result suggests that a DADTI-like molecule(s) is synthesized in rat intestinal goblet cells and is secreted in a diacrine fashion into the intestinal lumen at a late fetal period. The molecule(s) thus secreted may be important for the intestine of rats just before birth, because DADTI-like immunopositive goblet cells are no longer seen at any postnatal period.

Goblet cells and intestinal Alkaline phosphatase expression (IAP) during the development of the rat small intestine

2017 ◽  
Vol 119 (1) ◽  
pp. 71-77 ◽  
Author(s):  
José Rosa Gomes ◽  
Laís Costa Ayub ◽  
Camila Audrey dos Reis ◽  
Miriam Joice Machado ◽  
Jéssica da Silva ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Small Intestine ◽  
Goblet Cells ◽  
Rat Small Intestine ◽  
Intestinal Alkaline Phosphatase ◽  
Alkaline Phosphatase Expression
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