Kinetics of Intestinal Lamina propria Mast Cells, Globule Leucocytes, Intraepithelial Lymphocytes, Goblet Cells and Eosinophils in Murine Strongyloidiasis

ABSTRACTMMP-2 and MMP-9 are proteins with well stablished roles on the remodeling of tissue during morphogenesis. This study aimed to evaluate the activity and expression of the MMP-2 and MMP-9 in the rat small intestine mucosa layer on 15th and 18th days of intratuterine life (i.u.) and at 3rd, 10th, 17th, 25th, and 32th days after birth (a.b.). Samples were submitted to zimography, immunohistochemistry methods and Masson trichrome staining. Results showed that MMP-2 and MMP-9 were not expressed in the small intestine epithelium during intrauterine life. MMP-9 was immunolocalized in the villi goblet cells and in the lamina propria in cells identified as being the mast cells (a.b.). We concluded that in the i.u. and a.b. life the MMP-2 and MMP-9 were not expressed in the small intestine epithelium. However, after birth, because MMP-9 was expressed in the mast cels present in the lamina propria it may be involved in the remodeling process of the innate immunity that occurs during the small intestine development.

Download Full-text

Spontaneous release of interferon gamma by intestinal lamina propria lymphocytes in Crohn's disease. Kinetics of in vitro response to interferon gamma inducers.

Gut ◽

10.1136/gut.32.4.403 ◽

1991 ◽

Vol 32 (4) ◽

pp. 403-407 ◽

Cited By ~ 113

Author(s):

S Fais ◽

M R Capobianchi ◽

F Pallone ◽

P Di Marco ◽

M Boirivant ◽

...

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Lamina Propria ◽

Interferon Gamma ◽

Spontaneous Release ◽

Intestinal Lamina Propria ◽

In Vitro Response ◽

Kinetics Of ◽

Lamina Propria Lymphocytes

Download Full-text

Human intestinal lamina propria and intraepithelial lymphocytes express receptors specific for chemokines induced by inflammation

European Journal of Immunology ◽

10.1002/1521-4141(200003)30:3<819::aid-immu819>3.0.co;2-y ◽

2000 ◽

Vol 30 (3) ◽

pp. 819-826 ◽

Cited By ~ 102

Author(s):

William W. Agace ◽

Arthur I. Roberts ◽

Lijun Wu ◽

Colin Greineder ◽

Ellen C. Ebert ◽

...

Keyword(s):

Lamina Propria ◽

Intraepithelial Lymphocytes ◽

Intestinal Lamina Propria

Download Full-text

Neuropeptide AF Induces Piecemeal Degranulation in Murine Mucosal Mast Cells: A New Mediator in Neuro-Immune Communication in the Intestinal Lamina Propria?

The Anatomical Record ◽

10.1002/ar.23780 ◽

2018 ◽

Vol 301 (6) ◽

pp. 1103-1114 ◽

Cited By ~ 1

Author(s):

Nada Abdellah ◽

Samuel van Remoortel ◽

Omnia Mohey-Elsaeed ◽

Mohamed-Nabil Mustafa ◽

Yasser A. Ahmed ◽

...

Keyword(s):

Mast Cells ◽

Lamina Propria ◽

Intestinal Lamina Propria ◽

Mucosal Mast Cells

Download Full-text

Partial characterization of eosinophilic granule cells (EGCs) and identification of mast cells of the intestinal lamina propria in rainbow trout (Oncorhynchus mykiss). Biochemical and cytochemical study

Biology of the Cell ◽

10.1111/j.1768-322x.1995.tb00940.x ◽

1995 ◽

Vol 85 (1) ◽

pp. 35-41 ◽

Cited By ~ 15

Author(s):

Marie-France Sire ◽

Jean-Marie Vernier

Keyword(s):

Rainbow Trout ◽

Mast Cells ◽

Oncorhynchus Mykiss ◽

Lamina Propria ◽

Granule Cells ◽

Cytochemical Study ◽

Intestinal Lamina Propria ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Eosinophilic Granule

Download Full-text

FREQUENCY AND DISTRIBUTION OF IMMUNOGLOBULIN CONTAINING PLASMA CELLS IN ILEUM REPRESENTING GUT-ASSOCIATED-LYMPHATIC TISSUES IN THE BCRDV-VACCINATED BROILER

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v10i1-2.15641 ◽

2013 ◽

Vol 10 (1-2) ◽

pp. 15-20

Author(s):

M. A. Masum ◽

M. Z. I. Khan ◽

M. N. H. Siddiqi ◽

M. Nasrin ◽

N. Sultana

Keyword(s):

Lamina Propria ◽

Broiler Chickens ◽

Plasma Cells ◽

Goblet Cells ◽

Intraepithelial Lymphocytes ◽

Lymphatic Tissue ◽

Histological Studies ◽

The Core ◽

And Control ◽

Columnar Cells

Histological studies and development of immunoglobulin containing plasma cells in ileum representing gut-associated-lymphatic tissue of various group of broiler chickens vaccinated with Baby Chick Ranikhet Disease Vaccine (BCRDV) was carried out in the Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh during January to October, 2011. A total of 24 day-old broiler chickens of both sexes were divided into two groups such as vaccinated and control. Vaccinated group of broiler chickens were vaccinated at third day through ocular route. It was observed that the villi of ileum were long and slender, lined by simple columnar cells with numerous goblet cells. Scattered lymphocytes and unorganized lymphatic nodules were present in the lamina propria and core of the villi. The frequency and distribution of lymphocytes in the lamina propria and intraepithelial lymphocytes were increased with concentration in vaccinated group. The length and width of mucosa were highest at D28. In the ileum of chickens, the IgA, IgG and IgM positive cells were found in the lamina propria, around the intestinal glands and in the core of the villi of both control and vaccinated group of chickens. The IgA positive cells were significantly (p<0.01) higher than IgG and IgM positive cells in all stages of study in each group of chickens.DOI: http://dx.doi.org/10.3329/bjvm.v10i1-2.15641

Download Full-text

The protease phenotype and crystalline nature of the granules of intraepithelial mast cells in Trichinella spiralis-infected mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100140464 ◽

1995 ◽

Vol 53 ◽

pp. 818-819

Author(s):

D.S. Friend ◽

N. Ghildyal ◽

M.F. Gurish ◽

K.F. Austen ◽

R.L. Stevens

Keyword(s):

Small Intestine ◽

Mast Cells ◽

Epithelial Cells ◽

Lamina Propria ◽

Trichinella Spiralis ◽

Intestinal Epithelial ◽

Carboxypeptidase A ◽

C3h Mice ◽

Granule Morphology ◽

Crystalline Nature

Trichinella spiralis induces a profound mastocytosis and eosinophilia in the small intestine of the infected mouse. Mouse mast cells (MC) store in their granules various combinations of at least five chymotryptic chymases [designated mouse MC protease (mMCP) 1 to 5], two tryptic proteases designated mMCP-6 and mMCP-7 and an exopeptidase, carboxypeptidase A (mMC-CPA). Using antipeptide, protease -specific antibodies to these MC granule proteases, immunohistochemistry was done to determine the distribution, number and protease phenotype of the MCs in the small intestine and spleen 10 to >60 days after Trichinella infection of BALB/c and C3H mice. TEM was performed to evaluate the granule morphology of the MCs between intestinal epithelial cells and in the lamina propria (mucosal MCs) and in the submucosa, muscle and serosa of the intestine (submucosal MCs).As noted in the table below, the number of submucosal MCs remained constant throughout the study. In contrast, on day 14, the number of MCs in the mucosa increased ~25 fold. Increased numbers of MCs were observed between epithelial cells in the mucosal crypts, in the lamina propria and to a lesser extent, between epithelial cells of the intestinal villi.

Download Full-text