scholarly journals Marine Cyanobacteria Tune Energy Transfer Efficiency in their Light-harvesting Antennae by Modifying Pigment Coupling

2019 ◽  
Author(s):  
Yuval Kolodny ◽  
Hagit Zer ◽  
Mor Propper ◽  
Shira Yochelis ◽  
Yossi Paltiel ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Light Harvesting ◽  
Vertical Mixing ◽  
Photosynthetic Apparatus ◽  
Emission Spectra ◽  
Energy Transfer Efficiency ◽  
Transfer Efficiency ◽  
Transfer Energy ◽  
Harvesting Process

AbstractPhotosynthetic organisms regulate energy transfer to fit to changes in environmental conditions. The biophysical principles underlying the flexibility and efficiency of energy transfer in the light-harvesting process are still not fully understood. Here we examine how energy transfer is regulatedin-vivo. We compare different acclimation states of the photosynthetic apparatus in a marine cyanobacterial species that is well adapted to vertical mixing of the ocean water column and identify a novel acclimation strategy for photosynthetic life under low light intensities. Antennae rods extend, as expected, increasing light absorption. Surprisingly, in contrast to what was known for plants and predicted by classic calculations, these longer rods transfer energy fasteri.e.more efficiently. The fluorescence lifetime and emission spectra dependence on temperature, at the range of 4-300K, suggests that energy transfer efficiency is tuned by modifying the energetic coupling strength between antennae pigments.

scholarly journals A photosynthetic antenna complex foregoes unity carotenoid-to-bacteriochlorophyll energy transfer efficiency to ensure photoprotection

2020 ◽  
Vol 117 (12) ◽  
pp. 6502-6508 ◽  
Author(s):  
Dariusz M. Niedzwiedzki ◽  
David J. K. Swainsbury ◽  
Daniel P. Canniffe ◽  
C. Neil Hunter ◽  
Andrew Hitchcock
Keyword(s):  
Energy Transfer ◽  
Transient Absorption ◽  
Light Harvesting ◽  
Protein Complexes ◽  
Fluorescence Emission ◽  
Energy Transfer Efficiency ◽  
Transfer Efficiency ◽  
Light Harvesting Complexes ◽  
Antenna Complex ◽  
Pigment Protein Complexes

Carotenoids play a number of important roles in photosynthesis, primarily providing light-harvesting and photoprotective energy dissipation functions within pigment–protein complexes. The carbon–carbon double bond (C=C) conjugation length of carotenoids (N), generally between 9 and 15, determines the carotenoid-to-(bacterio)chlorophyll [(B)Chl] energy transfer efficiency. Here we purified and spectroscopically characterized light-harvesting complex 2 (LH2) fromRhodobacter sphaeroidescontaining theN= 7 carotenoid zeta (ζ)-carotene, not previously incorporated within a natural antenna complex. Transient absorption and time-resolved fluorescence show that, relative to the lifetime of the S1state of ζ-carotene in solvent, the lifetime decreases ∼250-fold when ζ-carotene is incorporated within LH2, due to transfer of excitation energy to the B800 and B850 BChlsa. These measurements show that energy transfer proceeds with an efficiency of ∼100%, primarily via the S1→ Qxroute because the S1→ S0fluorescence emission of ζ-carotene overlaps almost perfectly with the Qxabsorption band of the BChls. However, transient absorption measurements performed on microsecond timescales reveal that, unlike the nativeN≥ 9 carotenoids normally utilized in light-harvesting complexes, ζ-carotene does not quench excited triplet states of BChla, likely due to elevation of the ζ-carotene triplet energy state above that of BChla. These findings provide insights into the coevolution of photosynthetic pigments and pigment–protein complexes. We propose that theN≥ 9 carotenoids found in light-harvesting antenna complexes represent a vital compromise that retains an acceptable level of energy transfer from carotenoids to (B)Chls while allowing acquisition of a new, essential function, namely, photoprotective quenching of harmful (B)Chl triplets.

scholarly journals Enhancement of Energy Transfer Efficiency with Structural Control of Multichromophore Light‐Harvesting Assembly

2020 ◽  
Vol 7 (20) ◽  
pp. 2001623
Author(s):  
Inhwan Oh ◽  
Hosoowi Lee ◽  
Tae Wu Kim ◽  
Chang Woo Kim ◽  
Sunhong Jun ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Structural Control ◽  
Light Harvesting ◽  
Energy Transfer Efficiency ◽  
Transfer Efficiency

Influence of Phospholipid Composition on Self-Assembly and Energy-Transfer Efficiency in Networks of Light-Harvesting 2 Complexes

2013 ◽  
Vol 117 (36) ◽  
pp. 10395-10404 ◽  
Author(s):  
Ayumi Sumino ◽  
Takehisa Dewa ◽  
Tomoyasu Noji ◽  
Yuki Nakano ◽  
Natsuko Watanabe ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Self Assembly ◽  
Light Harvesting ◽  
Phospholipid Composition ◽  
Energy Transfer Efficiency ◽  
Transfer Efficiency

scholarly journals Direct single-molecule measurements of phycocyanobilin photophysics in monomeric C-phycocyanin

2017 ◽  
Vol 114 (37) ◽  
pp. 9779-9784 ◽  
Author(s):  
Allison H. Squires ◽  
W. E. Moerner
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Rational Design ◽  
Light Adaptation ◽  
De Novo ◽  
Photosynthetic Apparatus ◽  
Emission Spectra ◽  
Free Solution ◽  
Surface Attachment

Phycobilisomes are highly organized pigment–protein antenna complexes found in the photosynthetic apparatus of cyanobacteria and rhodophyta that harvest solar energy and transport it to the reaction center. A detailed bottom-up model of pigment organization and energy transfer in phycobilisomes is essential to understanding photosynthesis in these organisms and informing rational design of artificial light-harvesting systems. In particular, heterogeneous photophysical behaviors of these proteins, which cannot be predicted de novo, may play an essential role in rapid light adaptation and photoprotection. Furthermore, the delicate architecture of these pigment–protein scaffolds sensitizes them to external perturbations, for example, surface attachment, which can be avoided by study in free solution or in vivo. Here, we present single-molecule characterization of C-phycocyanin (C-PC), a three-pigment biliprotein that self-assembles to form the midantenna rods of cyanobacterial phycobilisomes. Using the Anti-Brownian Electrokinetic (ABEL) trap to counteract Brownian motion of single particles in real time, we directly monitor the changing photophysical states of individual C-PC monomers from Spirulina platensis in free solution by simultaneous readout of their brightness, fluorescence anisotropy, fluorescence lifetime, and emission spectra. These include single-chromophore emission states for each of the three covalently bound phycocyanobilins, providing direct measurements of the spectra and photophysics of these chemically identical molecules in their native protein environment. We further show that a simple Förster resonant energy transfer (FRET) network model accurately predicts the observed photophysical states of C-PC and suggests highly variable quenching behavior of one of the chromophores, which should inform future studies of higher-order complexes.

Polarity-Tuned Energy Transfer Efficiency in Artificial Light-Harvesting Antennae Containing Carbonyl Carotenoids Peridinin and Fucoxanthin

2007 ◽  
Vol 111 (1) ◽  
pp. 467-476 ◽  
Author(s):  
Tomáš Polívka ◽  
Mathias Pellnor ◽  
Eurico Melo ◽  
Torbjörn Pascher ◽  
Villy Sundström ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Light Harvesting ◽  
Energy Transfer Efficiency ◽  
Transfer Efficiency ◽  
Artificial Light

scholarly journals Marine cyanobacteria tune energy transfer efficiency in their light‐harvesting antennae by modifying pigment coupling

FEBS Journal ◽  
2020 ◽  
Author(s):  
Yuval Kolodny ◽  
Hagit Zer ◽  
Mor Propper ◽  
Shira Yochelis ◽  
Yossi Paltiel ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Light Harvesting ◽  
Energy Transfer Efficiency ◽  
Transfer Efficiency ◽  
Marine Cyanobacteria

Artificial Light–harvesting Systems (LHSs) Based on Boron-difluoride (BF2) Hydrazone Complexes (BODIHYs)

2021 ◽  
Author(s):  
Vishwa Deepak Singh ◽  
Bhupendra Kumar Dwivedi ◽  
Yogesh Kumar ◽  
Shankar Pandey
Keyword(s):  
Energy Transfer ◽  
Light Harvesting ◽  
High Energy ◽  
Energy Transfer Efficiency ◽  
Transfer Efficiency ◽  
Artificial Light ◽  
Harvesting Systems ◽  
Boron Difluoride ◽  
Hydrazone Ligands ◽  
Hydrazone Complexes

In quest to develop artificial light–harvesting systems (LHSs) with high energy transfer efficiency hydrazone ligands L1–L2 and their –BF2 complexes (BODIHYs; B1 and B2) have been synthesized. Ligands L1, L2...

scholarly journals Efficient estimation of energy transfer efficiency in light-harvesting complexes

2012 ◽  
Vol 86 (1) ◽  
Author(s):  
A. Shabani ◽  
M. Mohseni ◽  
H. Rabitz ◽  
S. Lloyd
Keyword(s):  
Energy Transfer ◽  
Light Harvesting ◽  
Energy Transfer Efficiency ◽  
Efficient Estimation ◽  
Transfer Efficiency ◽  
Light Harvesting Complexes
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