scholarly journals Comparative genomics in Chlamydomonas and Plasmodium identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates

2013 ◽  
Vol 27 (10) ◽  
pp. 1198-1215 ◽  
Author(s):  
J. Ning ◽  
T. D. Otto ◽  
C. Pfander ◽  
F. Schwach ◽  
M. Brochet ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Nuclear Envelope ◽  
Sexual Reproduction ◽  
Envelope Protein ◽  
Protein Family

scholarly journals Mutational analyses of fs(1)Ya, an essential, developmentally regulated, nuclear envelope protein in Drosophila.

Genetics ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 1473-1481 ◽  
Author(s):  
J Liu ◽  
K Song ◽  
M F Wolfner
Keyword(s):  
Cell Cycle ◽  
Embryonic Development ◽  
Nuclear Envelope ◽  
Envelope Protein ◽  
Chromatin Condensation ◽  
Cell Cycle Control ◽  
Nuclear Lamina ◽  
Mitotic Division ◽  
Developmentally Regulated ◽  
Egg Maturation

Abstract The fs(1)Ya protein (YA) is an essential, maternally encoded, nuclear lamina protein that is under both developmental and cell cycle control. A strong Ya mutation results in early arrest of embryos. To define the function of YA in the nuclear envelope during early embryonic development, we characterized the phenotypes of four Ya mutants alleles and determined their molecular lesions. Ya mutant embryos arrest with abnormal nuclear envelopes prior to the first mitotic division; a proportion of embryos from two leaky Ya mutants proceed beyond this but arrest after several abnormal divisions. Ya unfertilized eggs contain nuclei of different sizes and condensation states, apparently due to abnormal fusion of the meiotic products immediately after meiosis. Lamin is localized at the periphery of the uncondensed nuclei in these eggs. These results suggest that YA function is required during and after egg maturation to facilitate proper chromatin condensation, rather than to allow a lamin-containing nuclear envelope to form. Two leaky Ya alleles that partially complement have lesions at opposite ends of the YA protein, suggesting that the N- and C-termini are important for YA function and that YA might interact with itself either directly or indirectly.

The Nuclear Envelope Protein Emerin and Its Interacting Proteins

2016 ◽  
pp. 1-9
Author(s):  
Carol M Collins ◽  
Kimbre A Nee ◽  
James M Holaska
Keyword(s):  
Nuclear Envelope ◽  
Envelope Protein ◽  
Interacting Proteins

scholarly journals Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli

Nucleus ◽  
2016 ◽  
Vol 7 (1) ◽  
pp. 68-83 ◽  
Author(s):  
Ayaka Matsumoto ◽  
Chiyomi Sakamoto ◽  
Haruka Matsumori ◽  
Jun Katahira ◽  
Yoko Yasuda ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Envelope Protein

A Maternally Encoded Nuclear Envelope Protein Required for Embryonic Mitosis in Drosophila

1991 ◽  
Vol 56 (0) ◽  
pp. 719-727 ◽  
Author(s):  
H. Lin ◽  
K. Song ◽  
G.A. Hutcheson ◽  
C.E. Goutte ◽  
M.F. Wolfner
Keyword(s):  
Nuclear Envelope ◽  
Envelope Protein

scholarly journals Comparative Genomics Suggests Primary Homothallism of Pneumocystis Species

mBio ◽  
2015 ◽  
Vol 6 (1) ◽  
Author(s):  
João M. G. C. F. Almeida ◽  
Ousmane H. Cissé ◽  
Álvaro Fonseca ◽  
Marco Pagni ◽  
Philippe M. Hauser
Keyword(s):  
Comparative Genomics ◽  
Sexual Reproduction ◽  
Mating Type ◽  
Fungal Pathogens ◽  
Pneumocystis Carinii ◽  
Severe Pneumonia ◽  
Genomic Region ◽  
Pneumocystis Jirovecii ◽  
Content Type

ABSTRACT   Pneumocystis species are fungal parasites of mammal lungs showing host specificity. Pneumocystis jirovecii colonizes humans and causes severe pneumonia in immunosuppressed individuals. In the absence of in vitro cultures, the life cycle of these fungi remains poorly known. Sexual reproduction probably occurs, but the system of this process and the mating type (MAT) genes involved are not characterized. In the present study, we used comparative genomics to investigate the issue in P. jirovecii and Pneumocystis carinii, the species infecting rats, as well as in their relative Taphrina deformans. We searched sex-related genes using 103 sequences from the relative Schizosaccharomyces pombe as queries. Genes homologous to several sex-related role categories were identified in all species investigated, further supporting sexuality in these organisms. Extensive in silico searches identified only three putative MAT genes in each species investigated (matMc, matMi, and matPi). In P. jirovecii, these genes clustered on the same contig, proving their contiguity in the genome. This organization seems compatible neither with heterothallism, because two different MAT loci on separate DNA molecules would have been detected, nor with secondary homothallism, because the latter involves generally more MAT genes. Consistently, we did not detect cis-acting sequences for mating type switching in secondary homothallism, and PCR revealed identical MAT genes in P. jirovecii isolates from six patients. A strong synteny of the genomic region surrounding the putative MAT genes exists between the two Pneumocystis species. Our results suggest the hypothesis that primary homothallism is the system of reproduction of Pneumocystis species and T. deformans. IMPORTANCE   Sexual reproduction among fungi can involve a single partner (homothallism) or two compatible partners (heterothallism). We investigated the issue in three pathogenic fungal relatives: Pneumocystis jirovecii, which causes severe pneumonia in immunocompromised humans; Pneumocystis carinii, which infects rats; and the plant pathogen Taphrina deformans. The nature, the number, and the organization within the genome of the genes involved in sexual reproduction were determined. The three species appeared to harbor a single genomic region gathering only three genes involved in sexual differentiation, an organization which is compatible with sexual reproduction involving a single partner. These findings illuminate the strategy adopted by fungal pathogens to infect their hosts.

scholarly journals Meiotic Chromosome Homology Search Involves Modifications of the Nuclear Envelope Protein Matefin/SUN-1

Cell ◽  
2009 ◽  
Vol 139 (5) ◽  
pp. 920-933 ◽  
Author(s):  
Alexandra M. Penkner ◽  
Alexandra Fridkin ◽  
Jiradet Gloggnitzer ◽  
Antoine Baudrimont ◽  
Thomas Machacek ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Envelope Protein ◽  
Meiotic Chromosome ◽  
Homology Search ◽  
Chromosome Homology
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