scholarly journals The Western Clawed Frog (Xenopus tropicalis): An Emerging Vertebrate Model for Developmental Genetics and Environmental Toxicology

2009 ◽  
Vol 2009 (9) ◽  
pp. pdb.emo131-pdb.emo131
Author(s):  
C. Showell ◽  
F. L. Conlon
Keyword(s):  
Environmental Toxicology ◽  
Xenopus Tropicalis ◽  
Developmental Genetics ◽  
Vertebrate Model ◽  
Clawed Frog

scholarly journals Xenopus tropicalis egg extracts provide insight into scaling of the mitotic spindle

2007 ◽  
Vol 176 (6) ◽  
pp. 765-770 ◽  
Author(s):  
Katherine S. Brown ◽  
Michael D. Blower ◽  
Thomas J. Maresca ◽  
Timothy C. Grammer ◽  
Richard M. Harland ◽  
...  
Keyword(s):  
Cell Biology ◽  
Model Organism ◽  
Xenopus Tropicalis ◽  
Developmental Genetics ◽  
African Clawed Frog ◽  
Egg Extracts ◽  
Sperm Nuclei ◽  
Dose Dependent ◽  
Clawed Frog ◽  
Insight Into

The African clawed frog Xenopus laevis has been instrumental to investigations of both development and cell biology, but the utility of this model organism for genetic and proteomic studies is limited by its long generation time and unsequenced pseudotetraploid genome. Xenopus tropicalis, which is a small, faster-breeding relative of X. laevis, has recently been adopted for research in developmental genetics and functional genomics, and has been chosen for genome sequencing. We show that X. tropicalis egg extracts reconstitute the fundamental cell cycle events of nuclear formation and bipolar spindle assembly around exogenously added sperm nuclei. Interestingly, X. tropicalis spindles were ∼30% shorter than X. laevis spindles, and mixing experiments revealed a dynamic, dose-dependent regulation of spindle size by cytoplasmic factors. Measurements of microtubule dynamics revealed that microtubules polymerized slower in X. tropicalis extracts compared to X. laevis, but that this difference is unlikely to account for differences in spindle size. Thus, in addition to expanding the range of developmental and cell biological experiments, the use of X. tropicalis provides novel insight into the complex mechanisms that govern spindle morphogenesis.

Identification and signaling characterization of four urotensin II receptor subtypes in the western clawed frog, Xenopus tropicalis

2020 ◽  
Vol 299 ◽  
pp. 113586
Author(s):  
Norifumi Konno ◽  
Moe Takano ◽  
Koichi Miura ◽  
Mikiya Miyazato ◽  
Tomoya Nakamachi ◽  
...  
Keyword(s):  
Xenopus Tropicalis ◽  
Receptor Subtypes ◽  
Urotensin Ii ◽  
Clawed Frog

scholarly journals The State of the Art of the Zebrafish Model for Toxicology and Toxicologic Pathology Research—Advantages and Current Limitations

2003 ◽  
Vol 31 (1_suppl) ◽  
pp. 62-87 ◽  
Author(s):  
Jan M. Spitsbergen ◽  
Michael L. Kent
Keyword(s):  
Molecular Genetic ◽  
Model System ◽  
Developmental Genetics ◽  
Zebrafish Model ◽  
Vertebrate Model ◽  
Pathologic Lesions ◽  
Minimal Data ◽  
Advanced Knowledge ◽  
The Rich ◽  
Mutant Lines

The zebrafish (Danio rerio ) is now the pre-eminent vertebrate model system for clarification of the roles of specific genes and signaling pathways in development. The zebrafish genome will be completely sequenced within the next 1—2 years. Together with the substantial historical database regarding basic developmental biology, toxicology, and gene transfer, the rich foundation of molecular genetic and genomic data makes zebrafish a powerful model system for clarifying mechanisms in toxicity. In contrast to the highly advanced knowledge base on molecular developmental genetics in zebrafish, our database regarding infectious and noninfectious diseases and pathologic lesions in zebrafish lags far behind the information available on most other domestic mammalian and avian species, particularly rodents. Currently, minimal data are available regarding spontaneous neoplasm rates or spontaneous aging lesions in any of the commonly used wild-type or mutant lines of zebrafish. Therefore, to fully utilize the potential of zebrafish as an animal model for understanding human development, disease, and toxicology we must greatly advance our knowledge on zebrafish diseases and pathology.

scholarly journals Tissue Sampling and Genomic DNA Purification from the Western Clawed Frog Xenopus tropicalis

2009 ◽  
Vol 2009 (9) ◽  
pp. pdb.prot5294-pdb.prot5294 ◽  
Author(s):  
C. Showell ◽  
F. L. Conlon
Keyword(s):  
Genomic Dna ◽  
Xenopus Tropicalis ◽  
Dna Purification ◽  
Tissue Sampling ◽  
Clawed Frog

scholarly journals Temperature dependence of locomotor performance in the tropical clawed frog, Xenopus tropicalis

2012 ◽  
Vol 215 (14) ◽  
pp. 2465-2470 ◽  
Author(s):  
A. Herrel ◽  
C. Bonneaud
Keyword(s):  
Temperature Dependence ◽  
Xenopus Tropicalis ◽  
Locomotor Performance ◽  
Clawed Frog

scholarly journals Comparison of molecular and cellular events during lower jaw regeneration of newt (Cynops pyrrhogaster) and West African clawed frog (Xenopus tropicalis)

2007 ◽  
Vol 237 (2) ◽  
pp. 354-365 ◽  
Author(s):  
Hiroshi Kurosaka ◽  
Teruko Takano-Yamamoto ◽  
Takashi Yamashiro ◽  
Kiyokazu Agata
Keyword(s):  
West African ◽  
Xenopus Tropicalis ◽  
Cynops Pyrrhogaster ◽  
African Clawed Frog ◽  
Cellular Events ◽  
Lower Jaw ◽  
Clawed Frog

scholarly journals Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-19/20 Homologue in the IL-10 Locus

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Zhitao Qi ◽  
Qihuan Zhang ◽  
Zisheng Wang ◽  
Weihong Zhao ◽  
Qian Gao
Keyword(s):  
Amino Acids ◽  
Immune Response ◽  
Immune System ◽  
Genomic Structure ◽  
3D Structure ◽  
Interleukin 10 ◽  
Xenopus Tropicalis ◽  
African Clawed Frog ◽  
Clawed Frog ◽  
Sequence Identities

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays an important role in immune system. In the present study, the IL-10 gene of African clawed frog (Xenopus tropicalis) was first cloned, and its expression pattern and 3D structure were also analyzed. The frog IL-10 mRNA encoded 172 amino acids which possessed several conserved features found in IL-10s from other species, including five-exon/four-intron genomic structure, conserved four cysteine residues, IL-10 family motif, and sixα-helices. Real-time PCR showed that frog IL-10 mRNA was ubiquitous expressed in all examined tissues, highly in some immune related tissues including kidney, spleen, and intestine and lowly in heart, stomach, and liver. The frog IL-10 mRNA was upregulated at 24 h after LPS stimulation, indicating that it plays a part in the host immune response to bacterial infection. Another IL, termed as IL-20, was identified from the frog IL-10 locus, which might be the homologue of mammalian IL-19/20 according to the analysis results of the phylogenetic tree and the sequence identities.

scholarly journals Molecular footprinting of skeletal tissues in the catshark Scyliorhinus canicula and the clawed frog Xenopus tropicalis identifies conserved and derived features of vertebrate calcification

2015 ◽  
Vol 6 ◽  
Author(s):  
Sébastien Enault ◽  
David N. Muñoz ◽  
Willian T. A. F. Silva ◽  
Véronique Borday-Birraux ◽  
Morgane Bonade ◽  
...  
Keyword(s):  
Xenopus Tropicalis ◽  
Scyliorhinus Canicula ◽  
Clawed Frog

scholarly journals Cryptochrome Genes Are Highly Expressed in the Ovary of the African Clawed Frog, Xenopus tropicalis

PLoS ONE ◽  
2010 ◽  
Vol 5 (2) ◽  
pp. e9273 ◽  
Author(s):  
Yoko Kubo ◽  
Takahiro Takeuchi ◽  
Keiko Okano ◽  
Toshiyuki Okano
Keyword(s):  
Xenopus Tropicalis ◽  
African Clawed Frog ◽  
Clawed Frog
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