Enzymatic Synthesis of Multi-Milligram Quantities of Large, Linear DNA Molecules for Structural Studies

2009 ◽  
Vol 2009 (4) ◽  
pp. pdb.prot5197-pdb.prot5197 ◽  
Author(s):  
D. Jeruzalmi
Keyword(s):  
Enzymatic Synthesis ◽  
Structural Studies ◽  
Dna Molecules ◽  
Linear Dna

scholarly journals Preparation of Multimilligram Quantities of Large, Linear DNA Molecules for Structural Studies

Structure ◽  
2009 ◽  
Vol 17 (12) ◽  
pp. 1679
Author(s):  
Merlind Muecke ◽  
Martin Samuels ◽  
Megan Davey ◽  
David Jeruzalmi
Keyword(s):  
Structural Studies ◽  
Dna Molecules ◽  
Linear Dna

scholarly journals Preparation of Multimilligram Quantities of Large, Linear DNA Molecules for Structural Studies

Structure ◽  
2008 ◽  
Vol 16 (6) ◽  
pp. 837-841 ◽  
Author(s):  
Merlind Muecke ◽  
Martin Samuels ◽  
Megan Davey ◽  
David Jeruzalmi
Keyword(s):  
Structural Studies ◽  
Dna Molecules ◽  
Linear Dna

scholarly journals Topology-dependent anomalous dynamics of ring and linear DNA are sensitive to cytoskeleton crosslinking

2019 ◽  
Vol 5 (12) ◽  
pp. eaay5912 ◽  
Author(s):  
Devynn M. Wulstein ◽  
Kathryn E. Regan ◽  
Jonathan Garamella ◽  
Ryan J. McGorty ◽  
Rae M. Robertson-Anderson
Keyword(s):  
Transport Properties ◽  
Single Molecule ◽  
Intracellular Transport ◽  
Conformational Stability ◽  
Conformational Dynamics ◽  
Single Mode ◽  
Dna Molecules ◽  
Linear Dna ◽  
Linear Chains ◽  
Anomalous Dynamics

Cytoskeletal crowding plays a key role in the diffusion of DNA molecules through the cell, acting as a barrier to effective intracellular transport and conformational stability required for processes such as transfection, viral infection, and gene therapy. Here, we elucidate the transport properties and conformational dynamics of linear and ring DNA molecules diffusing through entangled and crosslinked composite networks of actin and microtubules. We couple single-molecule conformational tracking with differential dynamic microscopy to reveal that ring and linear DNA exhibit unexpectedly distinct transport properties that are influenced differently by cytoskeleton crosslinking. Ring DNA coils are swollen and undergo heterogeneous and biphasic subdiffusion that is hindered by crosslinking. Conversely, crosslinking actually facilitates the single-mode subdiffusion that compacted linear chains exhibit. Our collective results demonstrate that transient threading by cytoskeleton filaments plays a key role in the dynamics of ring DNA, whereas the mobility of the cytoskeleton dictates transport of linear DNA.

scholarly journals Functional properties, structural studies and chemo-enzymatic synthesis of oligosaccharides

2017 ◽  
Vol 66 ◽  
pp. 135-145 ◽  
Author(s):  
Chao Zhao ◽  
Yijing Wu ◽  
Xiaoyan Liu ◽  
Bin Liu ◽  
Hui Cao ◽  
...  
Keyword(s):  
Functional Properties ◽  
Enzymatic Synthesis ◽  
Structural Studies

scholarly journals Replication forms of the gene-sized DNA molecules of hypotrichous ciliates.

1983 ◽  
Vol 3 (9) ◽  
pp. 1562-1566 ◽  
Author(s):  
K G Murti ◽  
D M Prescott
Keyword(s):  
Electron Microscopy ◽  
S Phase ◽  
Origin Of Replication ◽  
Dna Molecules ◽  
Linear Dna ◽  
Dna Molecule ◽  
Independent Unit

Using a method for obtaining DNA from 10 to 40 macronuclei for electron microscopy, we analyzed the structure of gene-sized, linear DNA molecules from S-phase macronuclei of two hypotrichous ciliates, Euplotes eurystomus and Styx sp. Three types of putative replicating intermediates were observed: (i) molecules with a bubble close to one end, (ii) molecules with single forks, and (iii) molecules with two forks. We conclude that: (i) each macronuclear DNA molecule replicates as an independent unit, (ii) the molecules contain an origin of replication close to one or both ends, and (iii) the mode of replication is bidirectional.

scholarly journals Looping Dynamics of Linear DNA Molecules and the Effect of DNA Curvature: A Study by Brownian Dynamics Simulation

1998 ◽  
Vol 74 (2) ◽  
pp. 773-779 ◽  
Author(s):  
Holger Merlitz ◽  
Karsten Rippe ◽  
Konstantin V. Klenin ◽  
Jörg Langowski
Keyword(s):  
Brownian Dynamics ◽  
Dynamics Simulation ◽  
Dna Curvature ◽  
Dna Molecules ◽  
Brownian Dynamics Simulation ◽  
Linear Dna

Fast Procedure To Distinguish Circular and Linear DNA Molecules in Pulsed Field Gel Electrophoresis

1996 ◽  
Vol 29 (5) ◽  
pp. 745-753 ◽  
Author(s):  
M. Báez-Camargo ◽  
L. Lopez-Canovas ◽  
A. M. Riveron ◽  
P. Chávez ◽  
E. Orozco
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Molecules ◽  
Pulsed Field ◽  
Linear Dna ◽  
Fast Procedure

scholarly journals Transient orientation of linear DNA molecules during pulsed-fleld gel electrophoresis

1987 ◽  
Vol 15 (23) ◽  
pp. 10031-10044 ◽  
Author(s):  
G. Holzwarth ◽  
Chad B. McKee ◽  
Susan Steiger ◽  
Glenn Crater
Keyword(s):  
Gel Electrophoresis ◽  
Dna Molecules ◽  
Linear Dna

Translocation of tetrahedral DNA nanostructures through a solid-state nanopore

Nanoscale ◽  
2019 ◽  
Vol 11 (13) ◽  
pp. 6263-6269 ◽  
Author(s):  
Xinjia Zhao ◽  
Ruiping Ma ◽  
Ying Hu ◽  
Xiaoyu Chen ◽  
Ruifen Dou ◽  
...  
Keyword(s):  
Solid State ◽  
Dna Nanostructures ◽  
Dna Molecules ◽  
Linear Dna

Translocation of tetrahedral DNA nanostructures (TDNs) and TDNs bonded to linear DNA molecules through a nanopore was first demonstrated.

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