Expression of the Agrobacterium rhizogenes rolC Gene in a Deciduous Forest Tree Alters Growth and Development and Leads to Stem Fasciation

ABSTRACT This study aimed to characterize the dynamics, structural changes and floristics of a Northern Minas Gerais Seasonally Deciduous Forest tree community, in a 5 year interval. In 2005, 10 (20 x 20m) plots were allocated. All trees (CBH ≥ 10 cm) were tagged and measured. A second census was carried out in 2010 in order to measure surviving, new recruits and dead trees. In 2005, 46 species were recorded, moving to 45 in 2010. No significant differences were found for Shannon - diversity (H’ = 2.62 nats ind-1 in 2005; H’ = 2.60 nats ind-1 in 2010) and Pielou eveness (J = 0.683 in 2005; J = 0.682 in 2010) in the interval. A total of 57 dead records (rate of 1.64% year-1) were found whereas 18 trees were recruited (rate of 0.53% year-1). Despite the higher mortality as compared to recruitment, the results suggest that the community remained stable in both structural and diversity terms in the interval considered.

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Cell-autonomous behavior of the rolC gene of Agrobacterium rhizogenes during leaf development: a visual assay for transposon excision in transgenic plants.

The Plant Cell ◽

10.1105/tpc.1.12.1157 ◽

1989 ◽

Vol 1 (12) ◽

pp. 1157-1164 ◽

Cited By ~ 34

Author(s):

A Spena ◽

R B Aalen ◽

S C Schulze

Keyword(s):

Transgenic Plants ◽

Agrobacterium Rhizogenes ◽

Leaf Development ◽

Rolc Gene ◽

Autonomous Behavior ◽

Visual Assay

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Molecular taxonomy of Indopiptadenia oudhensis (Brandis) Brenan (Leguminosae - Mimosoideae) - A threatened endemic monotypic genus

Current Botany ◽

10.25081/cb.2020.v11.5977 ◽

2020 ◽

pp. 28-33

Author(s):

Ashok Kumar Dhakad ◽

Vijay Vardhan Pandey ◽

Rahul Kumar ◽

Ajay Thakur ◽

Anup Chandra ◽

...

Keyword(s):

Deciduous Forest ◽

Molecular Level ◽

Molecular Taxonomy ◽

Germplasm Conservation ◽

Forest Tree ◽

Rbcl Gene ◽

Nucleotide Variation ◽

Population Status ◽

Its Phylogeny ◽

Rbcl Gene Sequences

Indopiptadenia oudhensis (Brandis) Brenan is a threatened and an endemic legume forest tree genus of tropical moist deciduous forest of lower foothills of Himalaya. As population status of the species is declining at alarming rate, molecular characterization and germplasm conservation is urgently needed. This is the first attempt to draw its phylogeny based on molecular level and evolutionary closeness with other legumes. Among three barcode genes i.e rbcL, matK and ITS, only rbcLaF-rbcLaR gave satisfactory amplification and proved that rbcL is still working well than other barcode genes and justifies the evolutionary affinities with other legume species. Based on nucleotide homeology, the species is closely related to Prosopis cineraria and demonstrated nucleotide variation at only one site (552; A>G). Based on rbcL gene sequences and phylogeny, its evolutionary linkages found similar to the species placed in subfamilies Mimosaoideae and Caesalpiniodeae. However, researchers working on legume phylogeny clearly mentioned that Indopiptadenia is a part of clade mimosoid of Caesalpinioideae based on morphology. Hence, Indopiptadenia oudhensis may be the connecting species between subfamilies Mimosaoideae and Caesalpiniodeae.

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