scholarly journals Division Plane Orientation Defects Revealed by a Synthetic Double Mutant Phenotype

2017 ◽  
Vol 176 (1) ◽  
pp. 418-431 ◽  
Author(s):  
Ricardo Mir ◽  
Victoria H. Morris ◽  
Henrik Buschmann ◽  
Carolyn G. Rasmussen
Keyword(s):  
Double Mutant ◽  
Mutant Phenotype ◽  
Division Plane ◽  
Plane Orientation ◽  
Division Plane Orientation

scholarly journals Proper division plane orientation and mitotic progression together allow normal growth of maize

2017 ◽  
Vol 114 (10) ◽  
pp. 2759-2764 ◽  
Author(s):  
Pablo Martinez ◽  
Anding Luo ◽  
Anne Sylvester ◽  
Carolyn G. Rasmussen
Keyword(s):  
Cell Cycle ◽  
Cell Biology ◽  
Cell Cycle Progression ◽  
Normal Growth ◽  
Mitotic Progression ◽  
Wild Type ◽  
Cycle Progression ◽  
Division Plane ◽  
Plane Orientation ◽  
Division Plane Orientation

How growth, microtubule dynamics, and cell-cycle progression are coordinated is one of the unsolved mysteries of cell biology. A maize mutant,tangled1, with known defects in growth and proper division plane orientation, and a recently characterized cell-cycle delay identified by time-lapse imaging, was used to clarify the relationship between growth, cell cycle, and proper division plane orientation. Thetangled1mutant was fully rescued by introduction of cortical division site localized TANGLED1-YFP. A CYCLIN1B destruction box was fused to TANGLED1-YFP to generate a line that mostly rescued the division plane defect but still showed cell-cycle delays when expressed in thetangled1mutant. Although an intermediate growth phenotype between wild-type and thetangled1mutant was expected, these partially rescued plants grew as well as wild-type siblings, indicating that mitotic progression delays alone do not alter overall growth. These data indicate that division plane orientation, together with proper cell-cycle progression, is critical for plant growth.

scholarly journals An overview of plant division-plane orientation

2018 ◽  
Vol 219 (2) ◽  
pp. 505-512 ◽  
Author(s):  
Carolyn G. Rasmussen ◽  
Marschal Bellinger
Keyword(s):  
Division Plane ◽  
Plane Orientation ◽  
Division Plane Orientation

scholarly journals Cell division plane orientation based on tensile stress in Arabidopsis thaliana

2016 ◽  
Vol 113 (30) ◽  
pp. E4294-E4303 ◽  
Author(s):  
Marion Louveaux ◽  
Jean-Daniel Julien ◽  
Vincent Mirabet ◽  
Arezki Boudaoud ◽  
Olivier Hamant
Keyword(s):  
Cell Division ◽  
Tensile Stress ◽  
Shoot Apex ◽  
Boundary Region ◽  
Differential Growth ◽  
Division Plane ◽  
Plane Orientation ◽  
Maximal Tension ◽  
Division Plane Orientation ◽  
Cell Division Plane

Cell geometry has long been proposed to play a key role in the orientation of symmetric cell division planes. In particular, the recently proposed Besson–Dumais rule generalizes Errera’s rule and predicts that cells divide along one of the local minima of plane area. However, this rule has been tested only on tissues with rather local spherical shape and homogeneous growth. Here, we tested the application of the Besson–Dumais rule to the divisions occurring in the Arabidopsis shoot apex, which contains domains with anisotropic curvature and differential growth. We found that the Besson–Dumais rule works well in the central part of the apex, but fails to account for cell division planes in the saddle-shaped boundary region. Because curvature anisotropy and differential growth prescribe directional tensile stress in that region, we tested the putative contribution of anisotropic stress fields to cell division plane orientation at the shoot apex. To do so, we compared two division rules: geometrical (new plane along the shortest path) and mechanical (new plane along maximal tension). The mechanical division rule reproduced the enrichment of long planes observed in the boundary region. Experimental perturbation of mechanical stress pattern further supported a contribution of anisotropic tensile stress in division plane orientation. Importantly, simulations of tissues growing in an isotropic stress field, and dividing along maximal tension, provided division plane distributions comparable to those obtained with the geometrical rule. We thus propose that division plane orientation by tensile stress offers a general rule for symmetric cell division in plants.

scholarly journals Arabidopsis α Aurora Kinases Function in Formative Cell Division Plane Orientation

2011 ◽  
Vol 23 (11) ◽  
pp. 4013-4024 ◽  
Author(s):  
Daniël Van Damme ◽  
Bert De Rybel ◽  
Gustavo Gudesblat ◽  
Dmitri Demidov ◽  
Wim Grunewald ◽  
...  
Keyword(s):  
Cell Division ◽  
Aurora Kinases ◽  
Division Plane ◽  
Plane Orientation ◽  
Division Plane Orientation ◽  
Cell Division Plane

IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation

Nature Plants ◽  
2021 ◽  
Author(s):  
Pratibha Kumari ◽  
Pradeep Dahiya ◽  
Pantelis Livanos ◽  
Luise Zergiebel ◽  
Malte Kölling ◽  
...  
Keyword(s):  
Preprophase Band ◽  
Band Formation ◽  
Division Plane ◽  
Plane Orientation ◽  
Division Plane Orientation ◽  
Iq67 Domain

scholarly journals Roles of Cortical Actin Microfilament Patterning in Division Plane Orientation in Plants

2013 ◽  
Vol 54 (9) ◽  
pp. 1491-1503 ◽  
Author(s):  
Kei H. Kojo ◽  
Takumi Higaki ◽  
Natsumaro Kutsuna ◽  
Yuya Yoshida ◽  
Hiroki Yasuhara ◽  
...  
Keyword(s):  
Cortical Actin ◽  
Division Plane ◽  
Actin Microfilament ◽  
Plane Orientation ◽  
Division Plane Orientation

scholarly journals Functional characterization of TANGLED1 interaction with PHRAGMOPLAST ORIENTING KINESIN1 during mitosis in Arabidopsis

2021 ◽  
Author(s):  
Carolyn Rasmussen ◽  
Victoria H. Morris ◽  
Alison M Mills
Keyword(s):  
Double Mutant ◽  
Functional Characterization ◽  
Amino Acid Residues ◽  
Division Plane ◽  
Division Site ◽  
Site Localization ◽  
Mutant Phenotypes ◽  
Division Plane Orientation ◽  
Synthetic Growth

Cell division requires spatial coordination to properly position the division plane. How division plane positioning contributes to plant growth remains unknown. Two unrelated microtubule binding proteins, TANGLED1 (TAN1) and AUXIN-INDUCED-IN-ROOT-CULTURES9 (AIR9), are together required for normal Arabidopsis growth and division. tan1 air9 double mutants have synthetic growth and division plane orientation defects while single mutants lack obvious defects. We show that the first 132 amino acids of TAN1 (TAN1(1-132)) rescue the tan1 air9 double mutant and localize to the division site during telophase. Loss of both rescue and division-site localization occurred when interaction between TAN1 and PHRAGMOPLAST ORIENTING KINESIN1 (POK1) was disrupted by replacing six amino acid residues with alanines in TAN1(1-132). However, full-length TAN1 with the same alanine substitutions significantly rescued the tan1 air9 double mutant and remained at the division site throughout mitosis, although its accumulation was reduced and phragmoplast positioning defects occurred. POK1 often fails to accumulate at the division site in tan1 air9 mutants, suggesting that both TAN1 and AIR9 stabilize POK1 there. Finally, a mitosis specific promoter driving TAN1 rescued the tan1 air9 double mutant phenotypes indicating that defects seen in the root differentiation zone reflect the loss of mitotic-specific TAN1 activity.

scholarly journals TANGLED1 mediates interactions between microtubules that may promote spindle organization and phragmoplast guidance to the division site in maize

2019 ◽  
Author(s):  
Pablo Martinez ◽  
Ram Dixit ◽  
Rachappa S. Balkunde ◽  
Seán E. O’Leary ◽  
Kenneth A. Brakke ◽  
...  
Keyword(s):  
Critical Role ◽  
Preprophase Band ◽  
Microtubule Organization ◽  
Division Plane ◽  
Plane Orientation ◽  
Division Site ◽  
Structural Framework ◽  
Cell Shapes ◽  
Division Plane Orientation

AbstractThe microtubule cytoskeleton serves as a dynamic structural framework for mitosis in eukaryotic cells. TANGLED1 (TAN1) is a microtubule-binding protein that localizes to the division site and mitotic microtubules and plays a critical role in division plane orientation in plants. Here, in vitro experiments demonstrate that TAN1 directly binds microtubules, mediating microtubule zippering or end-on microtubule interactions, depending on their contact angle. Maize tan1 mutant cells improperly position the preprophase band (PPB), which predicts the future division site. However, cell-shape-based modeling indicates that PPB positioning defects are likely a consequence of abnormal cell shapes and not due to TAN1 absence. Spindle defects in the tan1 mutant suggest that TAN1-mediated microtubule zippering may contribute to metaphase spindle organization. In telophase, co-localization of growing microtubules ends from the phragmoplast with TAN1 at the division site suggests that TAN1 interacts with microtubule tips end-on. Together, our results suggest that TAN1 contributes to spindle and phragmoplast microtubule organization to ensure proper division plane orientation.

scholarly journals TANGLED1 mediates microtubule interactions that may promote division plane positioning in maize

2020 ◽  
Vol 219 (8) ◽  
Author(s):  
Pablo Martinez ◽  
Ram Dixit ◽  
Rachappa S. Balkunde ◽  
Antonia Zhang ◽  
Seán E. O’Leary ◽  
...  
Keyword(s):  
Critical Role ◽  
Preprophase Band ◽  
Microtubule Organization ◽  
Division Plane ◽  
Plane Orientation ◽  
Division Site ◽  
Structural Framework ◽  
Cell Shapes ◽  
Division Plane Orientation

The microtubule cytoskeleton serves as a dynamic structural framework for mitosis in eukaryotic cells. TANGLED1 (TAN1) is a microtubule-binding protein that localizes to the division site and mitotic microtubules and plays a critical role in division plane orientation in plants. Here, in vitro experiments demonstrate that TAN1 directly binds microtubules, mediating microtubule zippering or end-on microtubule interactions, depending on their contact angle. Maize tan1 mutant cells improperly position the preprophase band (PPB), which predicts the future division site. However, cell shape–based modeling indicates that PPB positioning defects are likely a consequence of abnormal cell shapes and not due to TAN1 absence. In telophase, colocalization of growing microtubules ends from the phragmoplast with TAN1 at the division site suggests that TAN1 interacts with microtubule tips end-on. Together, our results suggest that TAN1 contributes to microtubule organization to ensure proper division plane orientation.

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