scholarly journals The Use of 2,3,5-Triphenyl-Tetrazoliumchloride as a Measure of Seed Germinability

1947 ◽  
Vol 22 (2) ◽  
pp. 149-159 ◽  
Author(s):  
R. H. Porter ◽  
Mary Durrell ◽  
H. J. Romm
Keyword(s):  
Seed Germinability

A Simple Technique for Seed Transfer in X-ray Analysis of Seed

1974 ◽  
Vol 4 (3) ◽  
pp. 407-409
Author(s):  
B. S. P. Wang
Keyword(s):  
Simple Technique ◽  
Seedling Vigor ◽  
X Ray ◽  
Seed Germinability ◽  
Seed Transfer ◽  
Follow Up Study ◽  
Germinating Seeds ◽  
Tree Seed

Using X-radiography both for the follow-up study of germination and the correlation of tree seed germinability with embryo and endosperm (or megagametophyte) development often involve seed transfer by hand. Such a procedure is tedious and time-consuming and errors of seed displacement occur frequently. Conventional germination procedures also make counting germinants and evaluating seedling vigor difficult due to the shifting of positions of germinating seeds. A simple technique of using a combination of mechanized vacuum counting plate and perforated Plexiglas templates was devised to overcome these difficulties. It decreased by 40 times the time needed to transfer 400 seeds by hand. It also reduced errors of seed displacement.

Germination strategies under climate change scenarios along an aridity gradient

2020 ◽  
Vol 13 (4) ◽  
pp. 470-477
Author(s):  
Alexander Zogas ◽  
Evsey Kosman ◽  
Marcelo Sternberg
Keyword(s):  
Climate Change ◽  
Seed Banks ◽  
Arid Ecosystems ◽  
Annual Rainfall ◽  
Strong Impact ◽  
Climate Change Scenarios ◽  
Aridity Gradient ◽  
Soil Seed Banks ◽  
Seed Germinability ◽  
Semi Arid

Abstract Aims Climate change in the eastern Mediterranean region will have a strong impact on ecosystem functioning and plant community dynamics due to a reduction in annual rainfall and increased variability. We aim to understand the role of seed banks as potential buffers against climatic uncertainty determined by climate change. Methods We examined germination strategies of 18 common species present along an aridity gradient. Data were obtained from soil seed banks germinated during nine consecutive years from arid, semi-arid, Mediterranean and mesic Mediterranean ecosystems. At the semi-arid and Mediterranean sites, rainfall manipulations simulating 30% drought and 30% rainfall increase were applied. Germination strategies were tested under optimal irrigation conditions during three consecutive germination seasons to determine overall seed germinability in each soil sample. Changes in germination strategy were examined using a novel statistical approach that considers the climatic and biotic factors that may affect seed germinability. Important Findings The results showed that dominant species controlled their germination fractions by producing seeds with a different yearly germination fraction probability. The amount of rainfall under which the seeds were produced led to two major seed types with respect to germinability: high germinability, seeds leading to transient seed banks, and low germinability, seeds leading to persistent seed banks. We conclude that differential seed production among wet and dry years of both seed types creates a stable balance along the aridity gradient, enabling the soil seed bank to serve as a stabilizing mechanism buffering against rainfall unpredictability. Additionally, we present a general model of germination strategies of dominant annual species in Mediterranean and arid ecosystems that strengthens the notion of soil seed banks as buffers against climatic uncertainty induced by climate change in the region.

Changes in inorganic phosphate and seed germinability during afterripening of wild oats

1984 ◽  
Vol 62 (11) ◽  
pp. 2469-2471 ◽  
Author(s):  
William A. Quick ◽  
Andrew I. Hsiao
Keyword(s):  
Environmental History ◽  
Inorganic Phosphate ◽  
Room Temperature ◽  
Avena Fatua ◽  
Steady Increase ◽  
Wild Oat ◽  
Seed Germinability ◽  
Wild Oats

The period of afterripening required by dormant seeds of wild oat (Avena fatua L.) depends upon their genetic and environmental history. A steady increase was found in the level of inorganic phosphate (Pi) and germinability of dry mature caryopses of genetically dormant wild oat lines as afterripening progressed. There were no appreciable changes in Pi or in germinability of the companion seeds stored at −15 °C over the period of study. Secondary seeds were more dormant and had lower levels of Pi during afterripening than was the case with primary seeds. Storage at room temperature had little effect on Pi level of nondormant seed line. Results support the hypothesis that levels of endogenous Pi within the seed influence germinability.

Variation in pre-harvest sprouting tolerance and fresh seed germination in mungbean (Vigna radiata L.) genotypes

2017 ◽  
Vol 16 (5) ◽  
pp. 437-445 ◽  
Author(s):  
Amrit Lamichaney ◽  
Pradip Kumar Katiyar ◽  
Vijay Laxmi ◽  
Aditya Pratap
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Vigna Radiata ◽  
Biochemical Marker ◽  
Amylase Activity ◽  
Seed Germinability ◽  
Fresh Seed ◽  
Improvement Programme

AbstractMungbean seeds, despite being protected inside the pod, are susceptible to pre-harvest sprouting (PHS) following rainfall due to lack of fresh seed dormancy (FSD), which deteriorates the quality of the seed/grain produced. Therefore, development of mungbean cultivars with short (10–15 days) period of FSD has become important to curtail losses incurred by PHS. In this study, we investigated variations in PHS, fresh seed germination (FSG) and activity of α-amylase enzyme in diverse mungbean genotypes. There was a wide variation in PHS tolerance and FSG among 163 genotypes examined and 14 genotypes were found to be tolerant (<20%) to PHS. Seed germination in a pod, a measure used to evaluate PHS, varied from 7.14% in germplasm accession Chamu 4 to 82.52% in cultivated variety IPM 2–3. There was a marked increase in α-amylase activity in genotypes showing high FSG and PHS, especially at 48 and 72 h after germination as compared with PHS tolerant genotypes. Therefore, α-amylase can be used as an effective biochemical marker to evaluate a large number of mungbean genotypes for FSD and PHS. Also, the variation in seed germinability as found in this study could be further used for mungbean improvement programme.

Effects of ruminal and abomasal digestion on seed germinability of Japanese wild plants

2009 ◽  
Vol 55 (4) ◽  
pp. 225-229 ◽  
Author(s):  
Michihiro Obara ◽  
Shin-ichiro Ogura ◽  
Tetsuro Shishido ◽  
Kazuo Sugawara
Keyword(s):  
Wild Plants ◽  
Seed Germinability

scholarly journals Seed Characteristics of Wild Pear Genotypes from the in situ Population of the Banja Luka Region

2019 ◽  
Vol 20 (4) ◽  
Author(s):  
Nada Zavišić ◽  
Jelena Davidović Gidas ◽  
Borut Bosančić
Keyword(s):  
Fruit Production ◽  
Pyrus Communis ◽  
Seed Germinability ◽  
Mother Plants ◽  
Banja Luka ◽  
Seed Characteristics ◽  
Number Of Seeds ◽  
Tetrazolium Test

Wild pear (Pyrus communis var. piraster (L.) Ehrh.) is widely used as a generative rootstock for pear grafting. Seeds for the production of this rootstock should be taken from the registered mother plants. The aim of this research was to select mother plants in wild pear population from three localities (Banja Luka, Manjača, and Potkozarje). Fruit bearing regularity of selected trees was observed from 2012-2016, and the number of seeds per fruit was analysed in 2013, 2015 and 2016. Seed germinability and vitality analysis was carried out on 14 selected tress (genotypes). Seed germinability was tested according to the ISTA guideline. Seed vitality was determined by the tetrazolium test (TT). Accessions from Banja Luka locality expressed the highest fruit production. The highest number of seeds was determined in the genotypes marked as No. 6 and No. 21, amounting to 7.53 and 7.21 seeds, respectively. Seeds of the tested wild pears have shown good vitality. The highest seed vitality and germination of 92% was obtained from the accession No. 20. On the basis of the results, accessions marked with numbers: '6', '8', '20', '25', '31' and '32' can be used as mother plants for pear rootstock production.

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