Immunogold Localization of Pectin and Callose in Pollen Grains and Pollen Tubes of Brugmansia suaveolens — Implications for the Self-Incompatibility Reaction

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

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Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes.

The Plant Cell ◽

10.1105/tpc.3.3.271 ◽

1991 ◽

pp. 271-283 ◽

Cited By ~ 66

Author(s):

J. E. Gray ◽

B. A. McClure ◽

I. Bonig ◽

M. A. Anderson ◽

A. E. Clarke

Keyword(s):

Pollen Tubes ◽

The Self ◽

Nicotiana Alata ◽

Self Incompatibility

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Unilateral incompatibility and the effects of interspecific pollination for Erythronium americanum and Erythronium albidum (Liliaceae)

Canadian Journal of Botany ◽

10.1139/b93-038 ◽

1993 ◽

Vol 71 (2) ◽

pp. 353-358 ◽

Cited By ~ 41

Author(s):

Lawrence D. Harder ◽

Mitchell B. Cruzan ◽

James D. Thomson

Keyword(s):

Seed Production ◽

Pollen Tubes ◽

The Self ◽

Pollen Transfer ◽

Interspecific Incompatibility ◽

Unilateral Incompatibility ◽

Heterospecific Pollen ◽

Interspecific Pollen Transfer ◽

Erythronium Americanum ◽

Incompatibility Reaction

To determine whether interspecific pollen transfer could reduce seed production by two sympatric lilies, Erythronium albidum and Erythronium americanum, we hand-pollinated flowers with mixtures of conspecific and heterospecific pollen. These species exhibited typical unilateral interspecific incompatibility, i.e., pollen tubes from the self-infertile species (E. americanum) grew apparently unimpeded in styles of the self-fertile species (E. albidum), whereas the reverse cross resulted in an incompatibility reaction. Because of this asymmetrical relation and faster growth by heterospecific pollen tubes in E. albidum stigmas than by conspecific tubes, pollination with abundant heterospecific pollen reduced fruit and seed production by E. albidum, but not by E. americanum, as long as the stigma also received some conspecific pollen. Unilateral incompatibility could benefit self-infertile species in reproductive interactions with closely related self-fertile species; however, this benefit remains to be demonstrated for naturally pollinated plants. Key words: Erythronium albidum, Erythronium americanum, interspecific pollen transfer, pollination, unilateral incompatibility.

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Two Different Prunus SFB Alleles Have the Same Function in the Self-incompatibility Reaction

Plant Molecular Biology Reporter ◽

10.1007/s11105-012-0518-3 ◽

2012 ◽

Vol 31 (2) ◽

pp. 425-434 ◽

Cited By ~ 6

Author(s):

C. Gu ◽

J. Wu ◽

Y.-H. Du ◽

Y.-N. Yang ◽

S.-L. Zhang

Keyword(s):

The Self ◽

Self Incompatibility ◽

Incompatibility Reaction

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The formation of the tryphine coating the pollen grains of Raphanus , and its properties relating to the self-incompatibility system

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1973.0040 ◽

1973 ◽

Vol 184 (1075) ◽

pp. 149-165 ◽

Cited By ~ 95

Keyword(s):

Granular Material ◽

Pollen Grains ◽

The Self ◽

Self Incompatibility ◽

Pollen Maturation ◽

Incompatible Pollen ◽

Incompatibility System ◽

Two Stages ◽

Stimulation Of ◽

Incompatible Pollen Tube

The tryphine that coats the pollen grains of Raphanus is tapetally synthesized and is composed of a fibro-granular and a lipidic component. The fibro-granular material is proteinaceous and is secreted by cisternae of the endoplasmic reticulum. The lipidic component is derived, mainly, from degraded elaioplasts. The fibro-granular material is applied to the pollen exine first, followed by the lipidic mass. The tryphine condenses during the final stages of pollen maturation and dries down to form a thick, highly viscous coating. The major part of the condensation appears to result from dehydration. The tryphine, extracted from the pollen by a centrifugal method and mounted in a membrane, appears to be capable of penetrating the outer layers of a stigma of the same species and, if the pollen from which it was derived is incompatible with respect to the stigma, the stimulation of the production of the callosic reaction body in a manner similar to an incompatible pollen tube. It is proposed that, in Raphanus , substances responsible for the initiation of at least two stages in the self-incompatibility system are held in the tryphine.

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Pollen tube incompatibility reaction on the stigma in selfpollinated Sinapis alba L.

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1996.017 ◽

2014 ◽

Vol 65 (1-2) ◽

pp. 101-105 ◽

Cited By ~ 1

Author(s):

Renata Śnieżko ◽

Krystyna Winiarczyk

Keyword(s):

Pollen Tube ◽

Pollen Tube Growth ◽

Pollen Grains ◽

Pollen Tubes ◽

Sinapis Alba ◽

Pollen Grain ◽

Tube Growth ◽

Sinapis Alba L ◽

Growth Changes ◽

Incompatibility Reaction

After selfpollination of <em>Sinapis alba</em> L. pollen tubes growth is inhibited on the stigma. The pollen grains germinate 3-4 hours after pollination. The pollen give rise to one or more pollen tubes. They grow along the papillae. In the place of contact between the papilla and pollen tube the pellicula is digested. Then the direction of pollen tube growth changes completely. Pollen tubes grow back on the exine of their own pollen grain, or turn into the air. The pollen tubes growth was inhibited in 6-8 hours after selfpollination. After crosspollination usually there is no incompatibility reaction.

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The sensitivity of the pollen and stylar component of the self-incompatibility reaction to chronic gamma irradiation

Radiation Botany ◽

10.1016/0033-7560(75)90019-8 ◽

1975 ◽

Vol 15 (4) ◽

pp. 445-447 ◽

Cited By ~ 1

Author(s):

A.J.G. van Gastel ◽

D. de Nettancourt

Keyword(s):

Gamma Irradiation ◽

The Self ◽

Self Incompatibility ◽

Incompatibility Reaction

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Incompatibility Alleles in Portuguese Hazelnut Landraces

ISRN Agronomy ◽

10.5402/2012/154723 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6

Author(s):

Sandra Martins ◽

Mercè Rovira ◽

Ana Paula Silva ◽

Valdemar Carnide

Keyword(s):

Higher Plants ◽

Pollen Tubes ◽

The Self ◽

Single Locus ◽

Self Incompatibility ◽

Multiple Alleles ◽

Breeding Programmes ◽

Incompatibility Alleles ◽

Related Individuals ◽

New Cultivars

In many higher plants, selffertilization and genetically related individuals are prevented by pollen-stigma incompatibility. In the genus Corylus, incompatibility is of the sporophytic type and controlled by a single locus with multiple alleles. The objective of this study is to identify the S-alleles present in a collection of Portuguese landraces in order to select the most appropriate landraces for establishment of future orchards and for breeding programmes. Ten major Portuguese hazelnut landraces were submitted to controlled pollinations in the field, with 18 genotypes whose S-alleles are known. The pollen tubes were observed at 100X under a florescence microscope to evaluate their development. Three landraces were revealed to have S2 allele, two have S5, and four have one of the S3, S5, S10, and S18 alleles. One landrace was compatible with the 18 S-alleles tested and for two landraces, it was possible to identify both alleles. The information of the self-incompatibility relationship between these old cultivars is obviously useful for selecting the most suitable pollinators for planning new orchards and for new cultivars development.

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