Crystallization and preliminary X-ray diffraction studies of a Bowman–Birk inhibitor from Vigna unguiculata seeds
1999 ◽
Vol 55
(11)
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pp. 1920-1922
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Keyword(s):
X Ray
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A Bowman–Birk type trypsin/chymotrypsin inhibitor isolated from Vigna unguiculata seeds has been crystallized. Crystals were grown using the vapour-diffusion method at pH 4.0 using citrate/phosphate as a buffer and 30% saturated ammonium sulfate as precipitant. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 32.4, b = 61.8, c = 32.9 Å, β = 114.5°. The Matthews coefficient calculated assuming two molecules in the asymmetric unit was 1.95 Å3 Da−1, which corresponds to a 37% solvent content. X-ray data were collected to 2.5 Å resolution from a flash-frozen crystal. The structure was solved using the molecular-replacement method using tracy soybean inhibitor structure (PDB entry 1pi2) as a model.
2014 ◽
Vol 70
(4)
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pp. 485-488
2013 ◽
Vol 69
(4)
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pp. 355-357
2015 ◽
Vol 71
(4)
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pp. 466-470
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2014 ◽
Vol 70
(9)
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pp. 1244-1248
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2017 ◽
Vol 73
(11)
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pp. 601-606
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2014 ◽
Vol 70
(4)
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pp. 535-537
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2016 ◽
Vol 72
(9)
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pp. 667-671
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2014 ◽
Vol 70
(11)
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pp. 1566-1569