Large-Format Imaging Plate and Weissenberg Camera for Accurate Protein Crystallographic Data Collection Using Synchrotron Radiation

1997 ◽  
Vol 4 (3) ◽  
pp. 136-146 ◽  
Author(s):  
K. Sakabe ◽  
K. Sasaki ◽  
N. Watanabe ◽  
M. Suzuki ◽  
Z. G. Wang ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Data Collection ◽  
Crystallographic Data ◽  
Imaging Plate ◽  
Large Format

scholarly journals Hydrated metal(II) complexes of N-(6-amino-3,4-dihydro-3-methyl-5-nitroso-4-oxopyrimidin-2-yl) derivatives of glycine, glycylglycine, threonine, serine, valine and methionine: a monomeric complex and coordination polymers in one, two and three dimensions linked by hydrogen bonding. Corrigendum

2006 ◽  
Vol 62 (1) ◽  
pp. 165-165
Author(s):  
M. Luz Godino Salido ◽  
Paloma Arranz Mascarós ◽  
Rafaél López Garzón ◽  
M. Dolores Gutiérrez Valero ◽  
John N. Low ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Synchrotron Radiation ◽  
Data Collection ◽  
Coordination Polymers ◽  
Data Reduction ◽  
Three Dimensions ◽  
Compound Viii ◽  
Monomeric Complex ◽  
Cell Refinement ◽  
Derivatives Of

Some of the data collection details for compound (VIII) were incorrectly given in Table 1 of Godino Salido et al. (2004). The data for compound VIII in this paper were collected using synchrotron radiation at the Daresbury SRS station 9.8, λ = 0.6935 Å (Cernik et al., 1997; Clegg, 2000). The data were collected using a Bruker SMART 1K CCD diffractometer using ω rotation with narrow frames. The computer program used in the data collection was SMART (Bruker, 2001) and for cell refinement and data reduction SAINT (Bruker, 2001).

scholarly journals Protein microcrystallography using synchrotron radiation

IUCrJ ◽  
2017 ◽  
Vol 4 (5) ◽  
pp. 529-539 ◽  
Author(s):  
Masaki Yamamoto ◽  
Kunio Hirata ◽  
Keitaro Yamashita ◽  
Kazuya Hasegawa ◽  
Go Ueno ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Data Collection ◽  
Diffraction Data ◽  
Structure Determination ◽  
Structural Features ◽  
X Ray ◽  
Data Collection Strategy ◽  
Technological Developments ◽  
Novel Method

The progress in X-ray microbeam applications using synchrotron radiation is beneficial to structure determination from macromolecular microcrystals such as smallin mesocrystals. However, the high intensity of microbeams causes severe radiation damage, which worsens both the statistical quality of diffraction data and their resolution, and in the worst cases results in the failure of structure determination. Even in the event of successful structure determination, site-specific damage can lead to the misinterpretation of structural features. In order to overcome this issue, technological developments in sample handling and delivery, data-collection strategy and data processing have been made. For a few crystals with dimensions of the order of 10 µm, an elegant two-step scanning strategy works well. For smaller samples, the development of a novel method to analyze multiple isomorphous microcrystals was motivated by the success of serial femtosecond crystallography with X-ray free-electron lasers. This method overcame the radiation-dose limit in diffraction data collection by using a sufficient number of crystals. Here, important technologies and the future prospects for microcrystallography are discussed.

Advanced Crystallographic Data Collection Protocols for Experimental Phasing

2016 ◽  
pp. 175-191 ◽  
Author(s):  
Aaron D. Finke ◽  
Ezequiel Panepucci ◽  
Clemens Vonrhein ◽  
Meitian Wang ◽  
Gérard Bricogne ◽  
...  
Keyword(s):  
Data Collection ◽  
Crystallographic Data ◽  
Experimental Phasing

The Debye–Scherrer camera at synchrotron sources: a revisit

2012 ◽  
Vol 20 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Tine Straasø ◽  
Jacob Becker ◽  
Bo Brummerstedt Iversen ◽  
Jens Als-Nielsen
Keyword(s):  
High Resolution ◽  
Synchrotron Radiation ◽  
Powder Diffraction ◽  
Background Level ◽  
Cylindrical Sample ◽  
Entrance Window ◽  
Imaging Plate ◽  
High Resolution Imaging ◽  
Resolution Imaging ◽  
Imaging Plate Detector

In a powder diffraction pattern one measures the intensity of Miller-indexed Bragg peaksversusthe wavevector transfer sinθ/λ. With increasing wavevector transfer the density of occurrence of Bragg peaks increases while their intensity decreases until they vanish into the background level. The lowest possible background level is that due to Compton scattering from the powder. A powder diffraction instrument has been designed and tested that yields this ideal low-background level, obtainable by having the space between sample and detector all in vacuum with the entrance window so far upstream that scattering from it is negligible. To minimize overlap of Bragg peaks the combination of fine collimation of synchrotron radiation, a thin cylindrical sample and a high-resolution imaging plate detector is taken advantage of.

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