Expression, purification and crystallization of a dye-decolourizing peroxidase fromDictyostelium discoideum
2014 ◽
Vol 70
(2)
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pp. 252-255
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Keyword(s):
Dye-decolourizing peroxidases are haem-containing peroxidases with broad substrate specificity. Using H2O2as an electron acceptor, they efficiently decolourize various dyes that are of industrial and environmental relevance, such as anthraquninone- and azo-based dyes. In this study, the dye-decolourizing peroxidase DdDyP fromDictyostelium discoideumwas overexpressed inEscherichia colistrain Rosetta(DE3)pLysS, purified and crystallized using the vapour-diffusion method. A native crystal diffracted to 1.65 Å resolution and belonged to space groupP41212, with unit-cell parametersa=b= 141.03,c = 95.56 Å, α = β = γ = 90°. The asymmetric unit contains two molecules.
2012 ◽
Vol 68
(11)
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pp. 1351-1353
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2014 ◽
Vol 70
(11)
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pp. 1560-1562
2015 ◽
Vol 71
(3)
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pp. 354-357
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1999 ◽
Vol 55
(11)
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pp. 1946-1948
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2015 ◽
Vol 71
(8)
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pp. 1109-1113
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2016 ◽
Vol 72
(6)
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pp. 480-484
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