Recombinant ACHT1 fromArabidopsis thaliana: crystallization and X-ray crystallographic analysis
2017 ◽
Vol 73
(7)
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pp. 382-385
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Thioredoxins (Trxs) play important roles in chloroplasts by linking photosynthetic light reactions to a series of plastid functions. They execute their function by regulating the oxidation and reduction of disulfide bonds. ACHT1 (atypical cysteine/histidine-rich Trx1) is a thylakoid-associated thioredoxin-type protein found in theArabidopsis thalianachloroplast. Recombinant ACHT1 protein was overexpressed inEscherichia coli, purified and crystallized by the vapour-diffusion method. The crystal diffracted to 1.7 Å resolution and a complete X-ray data set was collected. Preliminary crystallographic analysis suggested that the crystals belonged to space groupC2221, with unit-cell parametersa = 102.7,b= 100.6,c= 92.8 Å.
2012 ◽
Vol 68
(11)
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pp. 1351-1353
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2014 ◽
Vol 70
(11)
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pp. 1560-1562
2015 ◽
Vol 71
(2)
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pp. 211-216
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2015 ◽
Vol 71
(8)
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pp. 1017-1019
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2015 ◽
Vol 71
(1)
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pp. 107-109
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2012 ◽
Vol 68
(2)
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pp. 244-246
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