A 2 × 2 circularly polarized (CP) sequential rotation microstrip patch antenna array with high gain for long-range ultra-high frequency (UHF) radio frequency identification (RFID) communication is proposed in this paper. In order to meet the operational frequency band requirement of 840–960 MHz and, at the same time, achieve enhanced broadside gain, a two-level sequential rotation structure is developed. Series power divider is used as the basic element of the feed network that is implemented with the substrate-integrated coaxial line technology for minimizing radiation losses. The manufactured prototype exhibits a peak gain of 12.5 dBic at 900 MHz and an axial ratio (AR) bandwidth (AR ≤ 3 dB) of 18.2% from 828 to 994 MHz. In comparison with the state-of-the-art, the proposed antenna shows an excellent gain/size trade-off.