An automatic procedure to search highly repetitive sequences in genome as fluorescence in situ hybridization probes and its application on Brachypodium distachyon

An analysis of the presence and distribution of the rye and wheat repeated sequences in rye B chromosomes was carried out by fluorescent in situ hybridization. Probes used consisted of three highly repetitive sequences from rye (pSc119.2, pSc74, and pSc34) and the multigene families for the 25S–5.8S–18S and 5S rDNA from wheat (pTa71 and pTa794, respectively). pSc74 and pSc119.2 showed hybridization signals in the telomeric regions of rye B chromosomes. The remaining DNA clones did not hybridize to the B chromosomes.Key words: Secale cereale, rye, repetitive DNA, fluorescence in situ hybridization, B chromosomes.

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Detection of structural chromosome damage in rat interphase cells using region-specific fluorescence in situ hybridization probes developed by microdissection

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/s0027-5107(98)00164-x ◽

1998 ◽

Vol 421 (2) ◽

pp. 179-190 ◽

Cited By ~ 1

Author(s):

Kyomu Matsumoto ◽

James D. Tucker

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Chromosome Damage ◽

Specific Fluorescence ◽

Hybridization Probes ◽

Interphase Cells ◽

Structural Chromosome

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Catch-linker + PCR labeling: a simple method to generate fluorescence in situ hybridization probes from yeast artificial chromosomes.

Genome Research ◽

10.1101/gr.4.4.209 ◽

1995 ◽

Vol 4 (4) ◽

pp. 209-211 ◽

Cited By ~ 4

Author(s):

Y Shibasaki ◽

J C Maule ◽

R S Devon ◽

E M Slorach ◽

J R Gosden ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Simple Method ◽

Artificial Chromosomes ◽

Hybridization Probes ◽

Yeast Artificial Chromosomes

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Faculty Opinions recommendation of False-negative rates for MYC fluorescence in situ hybridization probes in B-cell neoplasms.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.736451781.793564132 ◽

2019 ◽

Author(s):

Stefano Pileri

Keyword(s):

In Situ Hybridization ◽

B Cell ◽

Fluorescence In Situ Hybridization ◽

False Negative ◽

Hybridization Probes

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Isolation and characterization of a satellite DNA family in the Saccharum complex

Genome ◽

10.1139/g98-076 ◽

1998 ◽

Vol 41 (6) ◽

pp. 854-864 ◽

Cited By ~ 25

Author(s):

Karine Alix ◽

Franc-Christophe Baurens ◽

Florence Paulet ◽

Jean-Christophe Glaszmann ◽

Angélique D'Hont

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Satellite Dna ◽

Repetitive Sequences ◽

Repeat Sequence ◽

Miscanthus Sinensis ◽

Saccharum Complex ◽

Satellite Dnas ◽

Isolation And Characterization

EaCIR1, a 371-bp Erianthus-specific satellite DNA sequence, was cloned from TaqI restricted genomic DNA after agarose-gel electrophoresis. This sequence has 77% homology with a 365-bp satellite of Helictotrichon convolutum and 72% homology with a 353-bp tandem repeat sequence from Oryza sativa. PCR primers defined in the conserved regions of these repetitive sequences were used to isolate other satellite DNAs in different representatives of the Saccharum complex: SoCIR1 in Saccharum officinarum, SrCIR1 in Saccharum robustum, SsCIR1 and SsCIR2 in Saccharum spontaneum, and MsCIR1 in Miscanthus sinensis. EaCIR1 and SoCIR1 were localized to subtelomeric regions of the chromosomes by fluorescence in situ hybridization. Southern hybridization experiments, using two representatives of this repeat sequence family as probes, illustrated contrasting species-specificity and demonstrated the existence of similar repetitive elements in sorghum and maize.Key words: satellite DNA, sugarcane, Saccharum complex, Gramineae, fluorescence in situ hybridization, FISH.

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